Genome Sequence of the Agar-Degrading Marine Bacterium Alteromonadaceae sp. Strain G7

Kwak, Min Jung; Song, Ju Yeon; Kim, Byung Kwon; Chi, Won Jae; Kwon, Soon Kyeong; Choi, Soobeom; Chang, Yong Keun; Hong, Soon Kwang; Kim, Jihyun F.

doi:10.1128/jb.01931-12

Cited by 18 publications

(8 citation statements)

References 11 publications

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“…UCD-FRSSP16_17 [43]), seawater, and mussel Protothaca jedoensis (S. japonica [41]). Therefore, we hypothesized that in addition to the reported genera, including Microbulbifer [9], Flammeovirga [10], Alteromonadaceae [11], Bacillus [12], Vibrio [13], Forumosa [14], and Saccharophagus [15], some certain members of Shewanella could also serve as the novel centers of degradation and metabolism for complex seaweed polysaccharides in marine environments, suggesting the important ecological role of Shewanella for the marine carbon cycle process.…”

Section: Discussionmentioning

confidence: 99%

“…At least two properties of the strains need to be satisfied for simultaneously producing multiple seaweed oligosaccharides: (i) the potential industrial strains should possess high enzymic activities for the degradation of various seaweed polysaccharides; (ii) the optimal conditions of enzyme production and reaction for different polysaccharide degradation should be similar, which can reduce the complexity of the production technology of multiple seaweed oligosaccharides. Considering these requirements, although several microbial strains, including Microbulbifer [9], Flammeovirga [10], Alteromonadaceae [11], Bacillus [12], Vibrio [13], Forumosa [14], and Saccharophagus [15], have developed versatile degradability for multiple seaweed polysaccharides, these strains cannot serve as the industrial tools for multiple seaweed oligosaccharide production because of their unknown and unoptimized degradation characteristics.…”

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confidence: 99%

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The degradation activities for three seaweed polysaccharides ofShewanellasp. WPAGA9 isolated from deep‐sea sediments

Wang

Zeng

et al. 2021

J Basic Microbiol

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Seaweed oligosaccharides possess great bioactivities. However, different microbial strains are required to degrade multiple polysaccharides due to their limited biodegradability, thereby increasing the cost and complexity of production. Shewanella sp. WPAGA9 was isolated from deep-sea sediments in this study. According to the genomic and biochemical analyses, the extracellular fermentation broth of WPAGA9 had versatile degradation abilities for three typical seaweed polysaccharides including agar, carrageenan, and alginate. The maximum enzyme activities of the extracellular fermentation broth of WPAGA9 were 71.63, 76.4, and 735.13 U/ml for the degradation of agar, alginate, and carrageenan, respectively. Moreover, multiple seaweed oligosaccharides can be produced by the extracellular fermentation broth of WPAGA9 under similar optimum conditions. Therefore, WPAGA9 can simultaneously degrade three types of seaweed polysaccharides under similar conditions, thereby greatly reducing the production cost of seaweed oligosaccharides. This finding indicates that Shewanella sp. WPAGA9 is an ideal biochemical tool for producing multiple active seaweed oligosaccharides at low costs and is also an important participant in the carbon cycle process of the deep-sea environment.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

The degradation activities for three seaweed polysaccharides ofShewanellasp. WPAGA9 isolated from deep‐sea sediments

Wang

Zeng

et al. 2021

J Basic Microbiol

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“…4b; Table S7) might be detrimental to the host. Despite the possible deleterious impact of grazing on the algal host, the grazed G. firma can potentially be utilized as a bioresource for production of agar-derived biofuel due to its readily available reservoir of agarolytic bacteria (Kwak et al 2012). Moreover, polysaccharide-degrading bacteria isolated from grazed G. firma can potentially be applied in the degradation of algal waste (Satomi and Fujii 2014;Imran et al 2017).…”

Section: Discussionmentioning

confidence: 99%

The effect of grazing on the microbiome of two commercially important agarophytes, Gracilaria firma and G. salicornia (Gracilariaceae, Rhodophyta)

et al. 2020

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Grazing, which leads to losses in biomass and drastic declines in total crop production, is one of the main concerns in seaweed aquaculture. This is also thought to affect the composition of the associated bacterial communities which are believed to play a crucial role in determining the host's health and development. Apart from morphological impairment, studying changes in the prokaryotic microbiome composition and predicted functional responses to grazing will allow us to understand the underlying effects of grazing on the seaweed host. This study is the first report of the effect of grazing on the prokaryotic microbiome of two economically important agarophytes, Gracilaria firma and Gracilaria salicornia, by high-throughput sequencing targeting the V3-V4 variable region of the 16S rRNA gene. The results indicated that for G. firma, the microbiome composition of tissues grazed by marine herbivores had significantly more agarolytic bacteria Marinagarivorans sp. and Algisphaera sp. than in ungrazed tissues. The predictive functional metagenomics for this species revealed that grazing escalated the pathway activities related to nucleotide degradation, aromatic compound degradation and aerobic sugar metabolism, while pathways associated with cell wall synthesis, aerobic respiration, vitamin biosynthesis and amino acid biosynthesis were reduced. However, for G. salicornia, the bacterial communities were not significantly affected by grazing. Nevertheless, pathways relating to anaerobic respiration and amino acid, coenzyme and vitamin B-6 biosynthesis in this species were predicted to be more active in grazed tissues, whereas the microbiome of ungrazed tissues had higher activities in bacteriochlorophyll a, fatty acid, secondary metabolite and heme biosynthesis.

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“…The high abundance ratios of Colwelliaceae, Chromatiaceae and Alteromonadaceae observed in high salinity S7 may be attributed to their sensitivity to salinity (Fig. 3c), proving that they may belong to marine bacterium (Kwak et al 2012;Methé et al 2005;Pfennig Trüper 1981) and they may have "exclude salt" mechanisms such as osmotic balance and prevent dry (Banda et al 2020). In this adaptation, the microorganism synthesizes the corresponding solute to help stabilize the cell membrane structure.…”

Section: Effect Of Salinity On Bacterial Communitymentioning

confidence: 95%

Metagenomic Analysis of The Effects of Salinity On Microbial Diversity and Functional Gene Diversity in Kongsfjorden Estuary

Lin¹,

Wang²,

Han³

et al. 2021

Preprint

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Due to the inflow of meltwater from the Midre Lovénbreen glacier upstream of Kongsfjorden, the salinity of Kongsfjorden increases from the estuary to the interior of the fjord. Our goal was to determine which bacterial taxa and metabolism-related gene abundance were affected by changes in salinity, and whether salinity is correlated with genes related to nitrogen and sulfur cycling in fjord ecosystem using metagenomic analysis. Our data indicate that changes in salinity may affect some bacterial taxa, such as the relative abundance of Alphaproteobacteria and Deltaproteobacteria is higher at high salinity sites, while the relative abundance of Gammaproteobacteria and Betaproteobacteria is more dominant at low salinity sites. In addition, the relative abundance of some bacteria at the high and low salinity sites was different at the family level. For example, Rhodobacteraceae, Pseudoalteromonadaceae, Flavobacteriaceae, Vibrionaceae at the high salinity site Colwelliaceae, Chromatiaceae and Alteromonadaceae at the low salinity site are affected by salinity. In terms of functional gene diversity, our study proved that salinity could affect the relative abundance of related genes by affecting the metabolic mechanism of microorganisms. In addition to salinity, functional attributes of microorganisms themselves were also important factors affecting the relative abundance of metabolism-related genes. In addition, salinity has a certain effect on the relative abundance of genes related to nitrogen and sulfur cycling.

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Genome Sequence of the Agar-Degrading Marine Bacterium Alteromonadaceae sp. Strain G7

Cited by 18 publications

References 11 publications

The degradation activities for three seaweed polysaccharides ofShewanellasp. WPAGA9 isolated from deep‐sea sediments

The degradation activities for three seaweed polysaccharides ofShewanellasp. WPAGA9 isolated from deep‐sea sediments

The effect of grazing on the microbiome of two commercially important agarophytes, Gracilaria firma and G. salicornia (Gracilariaceae, Rhodophyta)

Metagenomic Analysis of The Effects of Salinity On Microbial Diversity and Functional Gene Diversity in Kongsfjorden Estuary

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