Genome Sequence of the Acetogenic Bacterium
            <i>Butyribacterium methylotrophicum</i>
            DSM 3468
            <sup>T</sup>

Bengelsdorf, Frank R.; Schiel-Bengelsdorf, Bettina; Daniel, Rolf; Dürre, Peter

doi:10.1128/genomea.01338-16

Cited by 14 publications

(10 citation statements)

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“…Few other bacteria outside of this genus have been reported to be able to produce butanol. However, genome sequences of Eubacterium limosum SA11 [ 41 ] as well as KIST612 [ 42 ] and Butyribacterium methylotrophicum [ 43 ] reveal that such microorganisms do not possess sol operons of either clostridial type. Instead, aldehyde and alcohol dehydrogenase genes are found, whose encoded enzymes catalyze the production of butanol from butyryl-CoA.…”

Section: Discussionmentioning

confidence: 99%

Microbial solvent formation revisited by comparative genome analysis

Poehlein

Solano

Flitsch

et al. 2017

Biotechnol Biofuels

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BackgroundMicrobial formation of acetone, isopropanol, and butanol is largely restricted to bacteria belonging to the genus Clostridium. This ability has been industrially exploited over the last 100 years. The solvents are important feedstocks for the chemical and biofuel industry. However, biological synthesis suffers from high substrate costs and competition from chemical synthesis supported by the low price of crude oil. To render the biotechnological production economically viable again, improvements in microbial and fermentation performance are necessary. However, no comprehensive comparisons of respective species and strains used and their specific abilities exist today.ResultsThe genomes of a total 30 saccharolytic Clostridium strains, representative of the species Clostridium acetobutylicum, C. aurantibutyricum, C. beijerinckii, C. diolis, C. felsineum, C. pasteurianum, C. puniceum, C. roseum, C. saccharobutylicum, and C. saccharoperbutylacetonicum, have been determined; 10 of them completely, and compared to 14 published genomes of other solvent-forming clostridia. Two major groups could be differentiated and several misclassified species were detected.ConclusionsOur findings represent a comprehensive study of phylogeny and taxonomy of clostridial solvent producers that highlights differences in energy conservation mechanisms and substrate utilization between strains, and allow for the first time a direct comparison of sequentially selected industrial strains at the genetic level. Detailed data mining is now possible, supporting the identification of new engineering targets for improved solvent production.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0742-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Microbial solvent formation revisited by comparative genome analysis

Poehlein

Solano

Flitsch

et al. 2017

Biotechnol Biofuels

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“…Hydrolyzed lactate seemed to be rapidly used for chain elongation since chain elongation products showed sustained production over time despite lactate monomers were depleted (Figure 2). Several bacteria harboring genes involved in lactate oxidation (lactate dehydrogenases, Ldh) and the RBO pathway such as C. tyrobutyricum (Wu et al, 2017), C. luticellarii (Poehlein et al, 2018) and members of the family Ruminococcaceae (Tao et al, 2017) including Caproiciproducens species (e.g., Bengelsdorf et al, 2019) were enriched in these conditions. C. tyrobutyricum is known as an efficient n-butyrate producer (Wu et al, 2017) but is not reported to produce longer carboxylates.…”

Section: Nzvi Affects Fermentation Conditions Shaping Microbiome Composition and Lactate Metabolismmentioning

confidence: 99%

nZVI Impacts Substrate Conversion and Microbiome Composition in Chain Elongation From D- and L-Lactate Substrates

Contreras-Dávila

Esveld

Buisman

et al. 2021

Front. Bioeng. Biotechnol.

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Medium-chain carboxylates (MCC) derived from biomass biorefining are attractive biochemicals to uncouple the production of a wide array of products from the use of non-renewable sources. Biological conversion of biomass-derived lactate during secondary fermentation can be steered to produce a variety of MCC through chain elongation. We explored the effects of zero-valent iron nanoparticles (nZVI) and lactate enantiomers on substrate consumption, product formation and microbiome composition in batch lactate-based chain elongation. In abiotic tests, nZVI supported chemical hydrolysis of lactate oligomers present in concentrated lactic acid. In fermentation experiments, nZVI created favorable conditions for either chain-elongating or propionate-producing microbiomes in a dose-dependent manner. Improved lactate conversion rates and n-caproate production were promoted at 0.5–2 g nZVI⋅L–1 while propionate formation became relevant at ≥ 3.5 g nZVI⋅L–1. Even-chain carboxylates (n-butyrate) were produced when using enantiopure and racemic lactate with lactate conversion rates increased in nZVI presence (1 g⋅L–1). Consumption of hydrogen and carbon dioxide was observed late in the incubations and correlated with acetate formation or substrate conversion to elongated products in the presence of nZVI. Lactate racemization was observed during chain elongation while isomerization to D-lactate was detected during propionate formation. Clostridium luticellarii, Caproiciproducens, and Ruminococcaceae related species were associated with n-valerate and n-caproate production while propionate was likely produced through the acrylate pathway by Clostridium novyi. The enrichment of different potential n-butyrate producers (Clostridium tyrobutyricum, Lachnospiraceae, Oscillibacter, Sedimentibacter) was affected by nZVI presence and concentrations. Possible theories and mechanisms underlying the effects of nZVI on substrate conversion and microbiome composition are discussed. An outlook is provided to integrate (bio)electrochemical systems to recycle (n)ZVI and provide an alternative reducing power agent as durable control method.

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“…Acetogens and their major characteristicsBernalier et al, 1996;Liu et al, 2008) Lesme et al, 1996 Liu et al, Zeikus et al, 1980;Lynd et al, 1982Bengelsdorf et al, 2016b) Abrini et al, 1994; K€ opke et al, 2011 Brown et al, 2014) CP010905 K€ opke et al, 2013 Riedel et al, 2015) K€ usel et al, 2000; Liou et al, 2005; G€ oßner et al, 2008; Jeong et al, 2014) Andreesen et al, 1970; Lux and Drake, 1992 Karl et al, 2017) Huhnke et al, 2008; K€ opke et al, 2011 Bengelsdorf et al, 2016a) Liou et al, 2005; Zhu et al, 2015)…”

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confidence: 99%

Using gas mixtures of CO, CO₂ and H₂ as microbial substrates: the do's and don'ts of successful technology transfer from laboratory to production scale

Takors

Kopf

Mampel

et al. 2018

Microbial Biotechnology

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107

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SummaryThe reduction of CO 2 emissions is a global effort which is not only supported by the society and politicians but also by the industry. Chemical producers worldwide follow the strategic goal to reduce CO 2 emissions by replacing existing fossil‐based production routes with sustainable alternatives. The smart use of CO and CO 2/H2 mixtures even allows to produce important chemical building blocks consuming the said gases as substrates in carboxydotrophic fermentations with acetogenic bacteria. However, existing industrial infrastructure and market demands impose constraints on microbes, bioprocesses and products that require careful consideration to ensure technical and economic success. The mini review provides scientific and industrial facets finally to enable the successful implementation of gas fermentation technologies in the industrial scale.

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Genome Sequence of the Acetogenic Bacterium Butyribacterium methylotrophicum DSM 3468 ^T

Abstract: Butyribacterium methylotrophicum DSM 3468T is an acetogenic methylotrophic, anaerobic, carbon monoxide–oxidizing bacterium that produces acetate, butyrate, and butanol. The genome consists of a single chromosome (4.3 Mb) and harbors 3,989 predicted protein-encoding genes.

Cited by 14 publications

References 14 publications

Microbial solvent formation revisited by comparative genome analysis

Microbial solvent formation revisited by comparative genome analysis

nZVI Impacts Substrate Conversion and Microbiome Composition in Chain Elongation From D- and L-Lactate Substrates

Using gas mixtures of CO, CO₂ and H₂ as microbial substrates: the do's and don'ts of successful technology transfer from laboratory to production scale

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Genome Sequence of the Acetogenic Bacterium Butyribacterium methylotrophicum DSM 3468 T

Abstract: Butyribacterium methylotrophicum DSM 3468T is an acetogenic methylotrophic, anaerobic, carbon monoxide–oxidizing bacterium that produces acetate, butyrate, and butanol. The genome consists of a single chromosome (4.3 Mb) and harbors 3,989 predicted protein-encoding genes.

Cited by 14 publications

References 14 publications

Microbial solvent formation revisited by comparative genome analysis

Microbial solvent formation revisited by comparative genome analysis

nZVI Impacts Substrate Conversion and Microbiome Composition in Chain Elongation From D- and L-Lactate Substrates

Using gas mixtures of CO, CO2 and H2 as microbial substrates: the do's and don'ts of successful technology transfer from laboratory to production scale

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Product

Resources

About

Genome Sequence of the Acetogenic Bacterium Butyribacterium methylotrophicum DSM 3468 ^T

Using gas mixtures of CO, CO₂ and H₂ as microbial substrates: the do's and don'ts of successful technology transfer from laboratory to production scale