Genome Sequence of Streptococcus agalactiae Strain 09mas018883, Isolated from a Swedish Cow

Zubair, Saima; Villiers, Etienne P. de; Fuxelius, Hans‐Henrik; Andersson, Gerhard; Johansson, K.-E.; Bishop, Richard P.; Bongcam‐Rudloff, Erik

doi:10.1128/genomea.00456-13

Cited by 10 publications

(8 citation statements)

References 15 publications

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“…commun.). Genome analysis of veterinary GBS strains reveals a considerable heterogeneity and shows that mechanisms of acquisition, duplication and reshuffling have permitted GBS to adapt to different environmental niches (Tettelin et al ., 2005; Delannoy et al ., 2012; Liu et al ., 2012; Pereira et al ., 2013; Rosinski-Chupin et al ., 2013; Wang et al ., 2013; Zubair et al ., 2013). …”

Section: Gbs Haemolysin and Pigmentmentioning

confidence: 99%

Group B streptococcal haemolysin and pigment, a tale of twins

Rosa-Fraile¹,

Dramsi²,

Spellerberg³

2014

FEMS Microbiol Rev

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Group B streptococcus [(GBS or Streptococcus agalactiae)] is a leading cause of neonatal meningitis and septicaemia. Most clinical isolates express simultaneously a β-haemolysin/cytolysin and a red polyenic pigment, two phenotypic traits important for GBS identification in medical microbiology. The genetic determinants encoding the GBS haemolysin and pigment have been elucidated and the molecular structure of the pigment has been determined. The cyl operon involved in haemolysin and pigment production is regulated by the major two-component system CovS/R, which coordinates the expression of multiple virulence factors of GBS. Genetic analyses indicated strongly that the haemolysin activity was due to a cytolytic toxin encoded by cylE. However, the biochemical nature of the GBS haemolysin has remained elusive for almost a century because of its instability during purification procedures. Recently, it has been suggested that the haemolytic and cytolytic activity of GBS is due to the ornithine rhamnopolyenic pigment and not to the CylE protein. Here we review and summarize our current knowledge of the genetics, regulation and biochemistry of these twin GBS phenotypic traits, including their functions as GBS virulence factors.

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Section: Gbs Haemolysin and Pigmentmentioning

confidence: 99%

Group B streptococcal haemolysin and pigment, a tale of twins

Rosa-Fraile¹,

Dramsi²,

Spellerberg³

2014

FEMS Microbiol Rev

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“…Compared with GBS strains whose complete genomes are available from the National Center for Biotechnology Information (NCBI), strain SGBS001 was most similar to the serotype V Swedish cow mastitis strain 09mas018883 (also ST-1; Fig. 1B) (23). Strain SGBS001 is >99% similar and has >99% coverage compared with strain 09mas018883, with the major difference being the presence of a ∼40 kb region uniquely in strain 09mas018883 (located between homologs of rdf_1233 and rdf_1234), which includes genes encoding proteins involved in lactose utilization (the lac.2 operon; Fig.…”

Section: Determination Of a Complete Genome Sequence Of An St-1 Gbs Smentioning

confidence: 99%

Sequence type 1 group B Streptococcus , an emerging cause of invasive disease in adults, evolves by small genetic changes

Galloway-Peña

Sahasrabhojane

Saldaña

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

101

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The molecular mechanisms underlying pathogen emergence in humans is a critical but poorly understood area of microbiologic investigation. Serotype V group B Streptococcus (GBS) was first isolated from humans in 1975, and rates of invasive serotype V GBS disease significantly increased starting in the early 1990s. We found that 210 of 229 serotype V GBS strains (92%) isolated from the bloodstream of nonpregnant adults in the United States and Canada between 1992 and 2013 were multilocus sequence type (ST) 1. Elucidation of the complete genome of a 1992 ST-1 strain revealed that this strain had the highest homology with a GBS strain causing cow mastitis and that the 1992 ST-1 strain differed from serotype V strains isolated in the late 1970s by acquisition of cell surface proteins and antimicrobial resistance determinants. Whole-genome comparison of 202 invasive ST-1 strains detected significant recombination in only eight strains. The remaining 194 strains differed by an average of 97 SNPs. Phylogenetic analysis revealed a temporally dependent mode of genetic diversification consistent with the emergence in the 1990s of ST-1 GBS as major agents of human disease. Thirty-one loci were identified as being under positive selective pressure, and mutations at loci encoding polysaccharide capsule production proteins, regulators of pilus expression, and two-component gene regulatory systems were shown to affect the bacterial phenotype. These data reveal that phenotypic diversity among ST-1 GBS is mainly driven by small genetic changes rather than extensive recombination, thereby extending knowledge into how pathogens adapt to humans.Streptococcus agalactiae | pathogenesis | evolution | single nucleotide polymorphisms | surface protein

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“…Streptococcus agalactiae (group B streptococcus [GBS]) is a common Gram-positive colonizer of terrestrial and aquatic vertebrates, including humans, cows, rabbits, cats, dogs, horses, frogs, trout, dolphins, and seals ( 1 , 2 ). GBS is a leading cause of invasive infections in neonates ( 1 ) and is implicated in a number of animal diseases, including bovine mastitis ( 3 ). Characterization of the genetic diversity of GBS within the oral microbiota of animals, especially pets that interact closely with humans, is critical to understanding better the source of opportunistic pathogens and to documenting bacterial communities.…”

Section: Genome Announcementmentioning

confidence: 99%

“…It also includes 3 prophage-like elements and 1 clustered regularly interspaced short palindromic repeat. The genome is most closely related to S. agalactiae strains isolated from the blood of a human, S. agalactiae SS1 ( 1 ), and from bovine milk from a cow with mastitis, S. agalactiae 09mas018883 ( 3 ). These two strains differ in genetic distance from the canine S. agalactiae ST-1 by 0.016 and 0.027, respectively.…”

Section: Genome Announcementmentioning

confidence: 99%