Genome Sequence of Pseudomonas mandelii PD30

Formusa, Philip A.; Hsiang, Tom; Habash, Marc; Lee, Hung; Trevors, J. T.

doi:10.1128/genomea.00713-14

Cited by 7 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 3 shows the ANIb values of all the 24 pseudomonads used in this study, which were in good agreement with those of ANIm ( Figure S8 ). The highest ANIb values for TNT3 and TNT19 were observed when these isolates were compared to P. mandelii PD30 (denitrifying bacterium) [ 71 ] and P. frederiksbergensis AS1 (aromatic degrader) [ 72 ], respectively, both reaching near 86% identity (clearly below the 95% threshold). Regarding TNT11, the highest ANIb value was observed when it was compared to P. veronii 1YdBTEX2 (aromatics degrader) [ 68 ], reaching nearly 94% identity (very close to the 95% threshold).…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation

Cabrera

Márquez

Pérez-Donoso

2022

Genes

View full text Add to dashboard Cite

The nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. Since physicochemical methods for remediation are poorly effective, the use of microorganisms has gained interest as an alternative to restore TNT-contaminated sites. We previously demonstrated the high TNT-transforming capability of three novel Pseudomonas spp. isolated from Deception Island, Antarctica, which exceeded that of the well-characterized TNT-degrading bacterium Pseudomonas putida KT2440. In this study, a comparative genomic analysis was performed to search for the metabolic functions encoded in the genomes of these isolates that might explain their TNT-transforming phenotype, and also to look for differences with 21 other selected pseudomonads, including xenobiotics-degrading species. Comparative analysis of xenobiotic degradation pathways revealed that our isolates have the highest abundance of key enzymes related to the degradation of fluorobenzoate, TNT, and bisphenol A. Further comparisons considering only TNT-transforming pseudomonads revealed the presence of unique genes in these isolates that would likely participate directly in TNT-transformation, and others involved in the β-ketoadipate pathway for aromatic compound degradation. Lastly, the phylogenomic analysis suggested that these Antarctic isolates likely represent novel species of the genus Pseudomonas, which emphasizes their relevance as potential agents for the bioremediation of TNT and other xenobiotics.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation

Cabrera

Márquez

Pérez-Donoso

2022

Genes

View full text Add to dashboard Cite

show abstract

“…On the other hand, species isolated from Juan Carlos I Station were related to P. mandelii. P. mandelii is a psychrotolerant bacterium characterized by it s met ab o lic versatility, plasticity, and biotechnological potential [33]. This bacterium was first isolated from mineral water in France [34] and it was also identified in mineral water samples from South Korea, and soil samples from China, United States, and Canada [33].…”

Section: B Isolation Of Bacteria Producing Cold-active Enzymesmentioning

confidence: 99%

“…P. mandelii is a psychrotolerant bacterium characterized by it s met ab o lic versatility, plasticity, and biotechnological potential [33]. This bacterium was first isolated from mineral water in France [34] and it was also identified in mineral water samples from South Korea, and soil samples from China, United States, and Canada [33]. Previous studies have reported the isolation of P. mandelii from Antarctic soils in King George Island [22] and Deception Island [20].…”

Section: B Isolation Of Bacteria Producing Cold-active Enzymesmentioning

confidence: 99%

Pseudomonas strains from the Livingston Island, Antarctica: a source of cold-active hydrolytic enzymes

Rubiano-Labrador¹,

Acevedo-Barrios²,

Lazaro³

et al. 2022

Proceedings of the 20th LACCEI International Multi-Conference for Engineering, Education and Technology: “Education, Research A

View full text Add to dashboard Cite

Pseudomonas spp. is considered one of the most successful bacterial genera due to its plasticity and meta bo lic ve rsatility, which has allowed it to colonize different ecosystems, including Antarctica. The ability of Pseudomonas to adapt a n d survive in the hostile conditions of the Antarctic makes them a re se rvoir of enzymes that can be used in different biotechnological applications; however, research on this genus in Antarctica is still in its infancy. Therefore, the aim of this study was to isolate and characterise cold-adapted Pseudomonas from Livingston Island, Antarctica, and expl ore t h eir a bi l it y to produce cold-active hydrolytic enzymes. In the present study, we isolated seven cold-adapted bacteria related to the genus Pseudomonas. The isolated strains have the ability to produce hydrolytic enzymes. These results demonstrate that cold-adapted Pseudomonas from Antarctica are a promising source of coldactive enzymes with biotechnological potential.

show abstract

“…Discussion. For the first time P. mandelii was isolated from mineral waters in France [23] and Korea [24], soils in China [25], river sediments from the Aldan River in the Central Yakutia region of Siberia [26], and in potato crop waters in New Brunswick, Canada [27].…”

Section: F I G 3 Pyrogenic Activity Of Lps Preparations Of P Mandelii U1 After Administrationmentioning

confidence: 99%

Lipopolysaccharide of Pseudomonas mandelii, Isolated from Antarctica

Броварська¹,

Varbanets²,

Gladka³

et al. 2021

Mikrobiol. Z.

View full text Add to dashboard Cite

Representatives of the Pseudomonas mandelii species are able to exist and multiply in places where the temperature is constantly low. The optimum growth temperature for P. mandelii is 25–30°C, although this bacterium can grow at 4°C but not at 37°C. Therefore, P. mandelii is an excellent example of psychrotolerant bacterium which like psychrophilic bacteria is characterized by a number of structural and functional adaptations that facilitate survival at low temperatures. To understand these microorganisms’ role in Antarctica the characterization of its biopolymers is vital. One of these biopolymers is lipopolysaccharide (LPS), composition and structure of which are diagnostically significant. This determines the aim of the work – to isolate lipopolysaccharides from the cells of Antarctic strain of P. mandelii, grown at different temperatures, to characterize them chemically, and to study their functional and biological activity. Methods. The object of the study was Pseudomonas sp. U1, isolated from moss on Galindez Island in Antarctica. Lipopolysaccharides were extracted from dried cells by 45% phenol water solution at 65–68°С by Westphal and Jann method. The amount of carbohydrates was determined by phenol-sulfuric method. Carbohydrate content was determined in accordance to the calibration curve, which was built using glucose as a standard. The content of nucleic acids was determined by Spirin, protein − by Lowry method. Serological activity of LPS was investigated by double immunodiffusion in agar using the method of Ouchterlony. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAAG electrophoresis) was performed according to Laemmli. Results. As a result of phylogenetic analysis (programs ClustalX 2.1, Tree view, Mega v. 6.00) it was shown that the Antarctic bacterial strain Pseudomonas sp. U1 associated with green moss has a 99.4% homology with the type strain from the GenBank database NR024902 P. mandelii CIP 105273T. According to these data and proximity to the corresponding cluster of species, the studied isolate can be identified as P. mandelii. A characteristic feature of LPS isolated from P. mandelii cells, grown at different temperatures (20°C and 4°C) is their heterogeneity. This is evidenced by the data of the monosaccharide composition, electrophoretic distribution, which showed that P. mandelii produces S- and SR-forms of LPS, differed in the length of the O-specific polysaccharide chains. The R-form of LPS is also present, which does not contain an O-specific polysaccharide chains. Structural heterogeneity is also inherent in LPS lipid A. This is evidenced by the data of the fatty acid composition. In LPS grown at 4°C no unsaturated fatty acids were found, while such ones are synthesized in LPS of other bacteria grown in the cold, in response to a decrease in growth temperature. The study of the immunochemical properties of LPS was carried out using polyclonal O-antisera as antibodies, and LPS as antigens indicated that in homologous systems LPS exhibited serological activity. LPS obtained from P. mandelii U1 cells, grown at 20°C, had a complex antigenic composition and gave two clear lines of precipitation at a concentration of 1 mg/mL. LPS obtained from P. mandelii U1 cells, grown at 4°C, gave one line, which indicates their serological homogeneity. Conclusions. For the first time lipopolysaccharides were isolated from cells of P. mandelii U1, grown at 4°C and 20°С. A characteristic feature of these LPS is their heterogeneity. This is evidenced by the data of the monosaccharide and fatty acid composition, electrophoretic distribution, which showed that P. mandelii produces S- and SR-forms of LPS, differed in the length of the O-specific polysaccharide chains. LPS, obtained from cells, grown at different temperatures, are differed by serological activity.

show abstract

Genome Sequence of Pseudomonas mandelii PD30

Abstract: The genome sequence of Pseudomonas mandelii PD30 is reported in this announcement. The genes for the reduction of nitrate to dinitrogen were identified in the genome assembly and subsequently used in gene expression research.

Cited by 7 publications

References 6 publications

Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation

Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation

Pseudomonas strains from the Livingston Island, Antarctica: a source of cold-active hydrolytic enzymes

Lipopolysaccharide of Pseudomonas mandelii, Isolated from Antarctica

Contact Info

Product

Resources

About