Genome Sequence of Pseudomonas citronellolis SJTE-3, an Estrogen- and Polycyclic Aromatic Hydrocarbon-Degrading Bacterium

Zheng, Daning; Wang, Xiuli; Wang, Pingping; Peng, Wen; Ji, Nannan; Liang, Rubing

doi:10.1128/genomea.01373-16

Cited by 21 publications

(21 citation statements)

References 26 publications

(24 reference statements)

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“…Our results indicate that members of the families Rhodocyclaceae , mainly Thauera , and Sphingomonadaceae and members of the genus Gordonia represent key steroid degraders in activated sludge of municipal and industrial WWTPs. Supporting the validity of our findings, steroid-degrading Sphingomonas ( 55 ), Novosphingobium ( 56 ), Comamonas ( 57 ), Pseudomonas ( 58 ), and Gordonia ( 59 ) strains were isolated from a variety of WWTP samples. In addition, Thauera as well as Comamonas and Pseudomonas were the major testosterone degraders in anaerobic and aerobic enrichment cultures from a municipal WWTP, respectively ( 32 , 60 ).…”

Section: Discussionsupporting

confidence: 77%

Metagenomes Reveal Global Distribution of Bacterial Steroid Catabolism in Natural, Engineered, and Host Environments

Holert

Cardenas

Bergstrand

et al. 2018

mBio

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Steroids are abundant growth substrates for bacteria in natural, engineered, and host-associated environments. This study analyzed the distribution of the aerobic 9,10-seco steroid degradation pathway in 346 publically available metagenomes from diverse environments. Our results show that steroid-degrading bacteria are globally distributed and prevalent in particular environments, such as wastewater treatment plants, soil, plant rhizospheres, and the marine environment, including marine sponges. Genomic signature-based sequence binning recovered 45 metagenome-assembled genomes containing a majority of 9,10-seco pathway genes. Only Actinobacteria and Proteobacteria were identified as steroid degraders, but we identified several alpha- and gammaproteobacterial lineages not previously known to degrade steroids. Actino- and proteobacterial steroid degraders coexisted in wastewater, while soil and rhizosphere samples contained mostly actinobacterial ones. Actinobacterial steroid degraders were found in deep ocean samples, while mostly alpha- and gammaproteobacterial ones were found in other marine samples, including sponges. Isolation of steroid-degrading bacteria from sponges confirmed their presence. Phylogenetic analysis of key steroid degradation proteins suggested their biochemical novelty in genomes from sponges and other environments. This study shows that the ecological significance as well as taxonomic and biochemical diversity of bacterial steroid degradation has so far been largely underestimated, especially in the marine environment.

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Section: Discussionsupporting

confidence: 77%

Metagenomes Reveal Global Distribution of Bacterial Steroid Catabolism in Natural, Engineered, and Host Environments

Holert

Cardenas

Bergstrand

et al. 2018

mBio

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“…There were extensive variations in the carriage of AMR genes among members of the megaplasmid family, including two megaplasmids lacking AMR genes altogether. pRB16 was identified in P. citronellolis SJTE-3, an oestrogen and polycyclic aromatic hydrocarbon degrading bacterium isolated from active sludge at a wastewater treatment plant in China 35 . P. koreensis P19E3, harbouring the megaplasmid p1, was isolated from healthy marjoram (Origanum majorana) leaf material in an organic herb farm (Boppelsen, Switzerland) in 2014 17 .…”

Section: Discussionmentioning

confidence: 99%

A megaplasmid family driving dissemination of multidrug resistance in Pseudomonas

et al. 2020

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Multidrug resistance (MDR) represents a global threat to health. Here, we used whole genome sequencing to characterise Pseudomonas aeruginosa MDR clinical isolates from a hospital in Thailand. Using long-read sequence data we obtained complete sequences of two closely related megaplasmids (>420 kb) carrying large arrays of antibiotic resistance genes located in discrete, complex and dynamic resistance regions, and revealing evidence of extensive duplication and recombination events. A comprehensive pangenomic and phylogenomic analysis indicates that: 1) these large plasmids comprise an emerging family present in different members of the Pseudomonas genus, and associated with multiple sources (geographical, clinical or environmental); 2) the megaplasmids encode diverse niche-adaptive accessory traits, including multidrug resistance; 3) the accessory genome of the megaplasmid family is highly flexible and diverse. The history of the megaplasmid family, inferred from our analysis of the available database, suggests that members carrying multiple resistance genes date back to at least the 1970s.

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“…There were extensive variations in the carriage of AMR genes amongst members of the megaplasmid family, including two of the megaplasmids lacking AMR genes altogether , both isolated from non-aeruginosa species of Pseudomonas . The pRB16 megaplasmid was identified in P. citronellolis SJTE-3, an estrogen and polycyclic aromatic hydrocarbon degrading bacterium isolated from active sludge at a wastewater treatment plant in China (75) . P. koreensis P19E3, harbouring the megaplasmid p1, was isolated from healthy marjoram ( Origanum marjorana ) leaf material during an isolation survey on an organic herb farm (Boppelsen, Switzerland) in 2014 (64) .…”

Section: Discussionmentioning

confidence: 99%

A megaplasmid family responsible for dissemination of multidrug resistance inPseudomonas

Cazares

Moore

Grimes

et al. 2019

Preprint

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Multidrug resistance (MDR) represents a global threat to health. Although plasmids can play an important role in the dissemination of MDR, they have not been commonly linked to the emergence of antimicrobial resistance in the pathogen Pseudomonas aeruginosa . We used whole genome sequencing to characterize a collection of P. aeruginosa clinical isolates from a hospital in Thailand. Using long-read sequence data we obtained complete sequences of two closely related megaplasmids (>420 kb) carrying large arrays of antibiotic resistance genes located in discrete, complex and dynamic resistance regions, and revealing evidence of extensive duplication and recombination events. A comprehensive pangenomic and phylogenomic analysis indicated that 1) these large plasmids comprise a family present in different members of the Pseudomonas genus and associated with multiple sources (geographical, clinical or environmental); 2) the megaplasmids encode diverse niche-adaptive accessory traits, including multidrug resistance; 3) the pangenome of the megaplasmid family is highly flexible and diverse, comprising a substantial core genome (average of 48% of plasmid genes), but with individual members carrying large numbers of unique genes. The history of the megaplasmid family, inferred from our analysis of the available database, suggests that members carrying multiple resistance genes date back to at least the 1970s.

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Genome Sequence of Pseudomonas citronellolis SJTE-3, an Estrogen- and Polycyclic Aromatic Hydrocarbon-Degrading Bacterium

Cited by 21 publications

References 26 publications

Metagenomes Reveal Global Distribution of Bacterial Steroid Catabolism in Natural, Engineered, and Host Environments

Metagenomes Reveal Global Distribution of Bacterial Steroid Catabolism in Natural, Engineered, and Host Environments

A megaplasmid family driving dissemination of multidrug resistance in Pseudomonas

A megaplasmid family responsible for dissemination of multidrug resistance inPseudomonas

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