Genome Sequence of Pseudomonas azelaica Strain Aramco J

Mohamed, Magdy El‐Said; Garcı́a, José Luis; Martínez, Igor; Cerro, Carlos del; Nogales, Juan; Dı́az, Eduardo

doi:10.1128/genomea.00037-15

Cited by 8 publications

(2 citation statements)

References 7 publications

(10 reference statements)

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“…OprM efflux system that confers tolerance to high concentrations of 2HBP, which makes these strains promising hosts for heterologous expression of dsz genes and the development of biocatalysts more resistant to the toxic effect of 2HBP (Czechowska et al, 2013;García et al, 2014;El-Said Mohamed et al, 2015).…”

Section: Engineering the Host Cellmentioning

confidence: 99%

Metabolic and process engineering for biodesulfurization in Gram-negative bacteria

Martínez¹,

Mohamed

Santos

et al. 2017

Journal of Biotechnology

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Microbial desulfurization or biodesulfurization (BDS) is an attractive low-cost and environmentally friendly complementary technology to the hydrotreating chemical process based on the potential of certain bacteria to specifically remove sulfur from S-heterocyclic compounds of crude fuels that are recalcitrant to the chemical treatments. The 4S or Dsz sulfur specific pathway for dibenzothiophene (DBT) and alkyl-substituted DBTs, widely used as model S-heterocyclic compounds, has been extensively studied at the physiological, biochemical and genetic levels mainly in Gram-positive bacteria. Nevertheless, several Gram-negative bacteria have been also used in BDS because they are endowed with some properties, e.g., broad metabolic versatility and easy genetic and genomic manipulation, that make them suitable chassis for systems metabolic engineering strategies. A high number of recombinant bacteria, many of which are Pseudomonas strains, have been constructed to overcome the major bottlenecks of the desulfurization process, i.e., expression of the dsz operon, activity of the Dsz enzymes, retro-inhibition of the Dsz pathway, availability of reducing power, uptake-secretion of substrate and intermediates, tolerance to organic solvents and metals, and other host-specific limitations. However, to attain a BDS process with industrial applicability, it is necessary to apply all the knowledge and advances achieved at the genetic and metabolic levels to the process engineering level, i.e., kinetic modelling, scale-up of biphasic systems, enhancing mass transfer rates, biocatalyst separation, etc. The production of high-added value products derived from the organosulfur material present in oil can be regarded also as an economically viable process that has barely begun to be explored.

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Section: Engineering the Host Cellmentioning

confidence: 99%

Metabolic and process engineering for biodesulfurization in Gram-negative bacteria

Martínez¹,

Mohamed

Santos

et al. 2017

Journal of Biotechnology

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“…The genomes of P. nitroreducens HBP-1 as well as its close relative P. azelaica strain Aramco J were recently released as drafts, consisting of 212 and 91 contigs, respectively [15,16]. Unfortunately, although the draft genomes provided insights into the gene content and functional capacities of P. nitroreducens, their incompleteness hampered comparative genomics analyses and evaluations of the potential role of mobile genetic elements in their evolution.…”

Section: Introductionmentioning

confidence: 99%

Insights into Mobile Genetic Elements of the Biocide-Degrading Bacterium Pseudomonas nitroreducens HBP-1

Carraro

Sentchilo

Polák

et al. 2020

Genes

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The sewage sludge isolate Pseudomonas nitroreducens HBP-1 was the first bacterium known to completely degrade the fungicide 2-hydroxybiphenyl. PacBio and Illumina whole-genome sequencing revealed three circular DNA replicons: a chromosome and two plasmids. Plasmids were shown to code for putative adaptive functions such as heavy metal resistance, but with unclarified ability for self-transfer. About one-tenth of strain HBP-1′s chromosomal genes are likely of recent horizontal influx, being part of genomic islands, prophages and integrative and conjugative elements (ICEs). P. nitroreducens carries two large ICEs with different functional specialization, but with homologous core structures to the well-known ICEclc of Pseudomonas knackmussii B13. The variable regions of ICEPni1 (96 kb) code for, among others, heavy metal resistances and formaldehyde detoxification, whereas those of ICEPni2 (171 kb) encodes complete meta-cleavage pathways for catabolism of 2-hydroxybiphenyl and salicylate, a protocatechuate pathway and peripheral enzymes for 4-hydroxybenzoate, ferulate, vanillin and vanillate transformation. Both ICEs transferred at frequencies of 10−6–10−8 per P. nitroreducens HBP-1 donor into Pseudomonas putida, where they integrated site specifically into tRNAGly-gene targets, as expected. Our study highlights the underlying determinants and mechanisms driving dissemination of adaptive properties allowing bacterial strains to cope with polluted environments.

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Enzymology and Genetics of Biodesulfurization Process

2018

Biodesulfurization in Petroleum Refining

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Genome Sequence of Pseudomonas azelaica Strain Aramco J

Cited by 8 publications

References 7 publications

Metabolic and process engineering for biodesulfurization in Gram-negative bacteria

Metabolic and process engineering for biodesulfurization in Gram-negative bacteria

Insights into Mobile Genetic Elements of the Biocide-Degrading Bacterium Pseudomonas nitroreducens HBP-1

Enzymology and Genetics of Biodesulfurization Process

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