Genome Sequence of Pseudomonas aeruginosa DQ8, an Efficient Degrader of
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            -Alkanes and Polycyclic Aromatic Hydrocarbons

Gai, Zhonghui; Zhang, Zheng‐Zhi; Wang, Xiaoyu; Tao, Fei; Tang, Hongzhi; Xu, Ping

doi:10.1128/jb.01499-12

Cited by 13 publications

(12 citation statements)

References 11 publications

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“…None of the BTEX group was metabolized, however (Zhang et al 2011). Moreover, the genome sequencing of DQ8 showed the presence of an alk cluster, confirming the phenotypic observations (Gai et al 2012). Interestingly, the strain exhibited two diversified, well recognized for various bacteria, enzymatic strategies towards fluorene.…”

Section: Bacteria Able To Metabolize Both N-alkanes and Aromatic Hydrsupporting

confidence: 72%

“…Interestingly, the strain exhibited two diversified, well recognized for various bacteria, enzymatic strategies towards fluorene. It could oxidize the compound via both C9-monooxidation and C3,C4dioxidation (Gai et al 2012). P. aeruginosa strain W10 also preferentially utilized n-C 16 as well as naphthalene, phenanthrene, fluoranthene, and pyrene (Chebbi et al 2017).…”

Section: Bacteria Able To Metabolize Both N-alkanes and Aromatic Hydrmentioning

confidence: 99%

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Aerobic bacteria degrading both n-alkanes and aromatic hydrocarbons: an undervalued strategy for metabolic diversity and flexibility

2018

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Environmental pollution with petroleum toxic products has afflicted various ecosystems, causing devastating damage to natural habitats with serious economic implications. Some crude oil components may serve as growth substrates for microorganisms. A number of bacterial strains reveal metabolic capacities to biotransform various organic compounds. Some of the hydrocarbon degraders are highly biochemically specialized, while the others display a versatile metabolism and can utilize both saturated aliphatic and aromatic hydrocarbons. The extended catabolic profiles of the latter group have been subjected to systematic and complex studies relatively rarely thus far. Growing evidence shows that numerous bacteria produce broad biochemical activities towards different hydrocarbon types and such an enhanced metabolic potential can be found in many more species than the already well-known oil-degraders. These strains may play an important role in the removal of heterogeneous contamination. They are thus considered to be a promising solution in bioremediation applications. The main purpose of this article is to provide an overview of the current knowledge on aerobic bacteria involved in the mineralization or transformation of both n-alkanes and aromatic hydrocarbons. Variant scientific approaches enabling to evaluate these features on biochemical as well as genetic levels are presented. The distribution of multidegradative capabilities between bacterial taxa is systematically shown and the possibility of simultaneous transformation of complex hydrocarbon mixtures is discussed. Bioinformatic analysis of the currently available genetic data is employed to enable generation of phylogenetic relationships between environmental strain isolates belonging to the phyla Actinobacteria, Proteobacteria, and Firmicutes. The study proves that the co-occurrence of genes responsible for concomitant metabolic bioconversion reactions of structurally-diverse hydrocarbons is not unique among various systematic groups.

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Section: Bacteria Able To Metabolize Both N-alkanes and Aromatic Hydrsupporting

confidence: 72%

Section: Bacteria Able To Metabolize Both N-alkanes and Aromatic Hydrmentioning

confidence: 99%

Aerobic bacteria degrading both n-alkanes and aromatic hydrocarbons: an undervalued strategy for metabolic diversity and flexibility

2018

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“…Specifically, several Pseudomonas isolates including two species closely associated with 16S rRNA gene sequences detected in this study (P. stuzeri, and P. alcaligenes) have demonstrated abilities to degrade PAHs such as naphthalene and phenanthrene, linear and branched alkanes, and diesel range compounds (Takizawa et al 1994;Ramos et al 1995;Whyte et al 1997;Beal and Betts 2000;Bosch et al 2000;Filonov et al 2000; Barathi and Vasudevan 2001;Tian et al 2003;Yeo et al 2003;Kaczorek et al 2011;Rocha et al 2011;Tang et al 2011;Gai et al 2012;Zhang et al 2012). Pseudomonas was one of the dominant genera encapsulated in gels from Bakken shale wells and reintroduced to flowback fluids in laboratory experiments, which substantially improved organic carbon degradation rates (Aukema et al 2014;Strong et al 2014).…”

Section: Implications For Surface Releasesmentioning

confidence: 70%

Aerobic biodegradation of organic compounds in hydraulic fracturing fluids

et al. 2015

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Little is known of the attenuation of chemical mixtures created for hydraulic fracturing within the natural environment. A synthetic hydraulic fracturing fluid was developed from disclosed industry formulas and produced for laboratory experiments using commercial additives in use by Marcellus shale field crews. The experiments employed an internationally accepted standard method (OECD 301A) to evaluate aerobic biodegradation potential of the fluid mixture by monitoring the removal of dissolved organic carbon (DOC) from an aqueous solution by activated sludge and lake water microbial consortia for two substrate concentrations and four salinities. Microbial degradation removed from 57 % to more than 90 % of added DOC within 6.5 days, with higher removal efficiency at more dilute concentrations and little difference in overall removal extent between sludge and lake microbe treatments. The alcohols isopropanol and octanol were degraded to levels below detection limits while the solvent acetone accumulated in biological treatments through time. Salinity concentrations of 40 g/L or more completely inhibited degradation during the first 6.5 days of incubation with the synthetic hydraulic fracturing fluid even though communities were pre-acclimated to salt. Initially diverse microbial communities became dominated by 16S rRNA sequences affiliated with Pseudomonas and other Pseudomonadaceae after incubation with the synthetic fracturing fluid, taxa which may be involved in acetone production. These data expand our understanding of constraints on the biodegradation potential of organic compounds in hydraulic fracturing fluids under aerobic conditions in the event that they are accidentally released to surface waters and shallow soils.

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“…This ability of bacteria to degrade PAHs is due to a multicomponent enzyme system that generates hydroxylated metabolites followed by a series of reactions to achieve the final mineralization of these compounds (Kanaly and Harayama 2000, Haritash and Kaushik 2009, Zhang et al 2011). In the case of P. aeruginosa, its ability to synthesize rhamnolipids in addition to its oxygenase/dehydrogenase system stands out (Rahman et al 2003, Jouanneau et al 2006, Kumara et al 2006, Zhang et al 2011, Zhao et al 2011, Cao et al 2012, Gai et al 2012. In low and high molecular weight PAHs, the first step of PAH biotransformation performed by some bacteria is the generation of dihydrodiols through their oxygenase activity (Kanaly and Harayama 2000) as it occurs in fish.…”

Section: Introductionmentioning

confidence: 99%

TOXICOKINETIC AND TOXICODYNAMIC SYMBIOTIC INTERACTIONS AMONG INTESTINAL Pseudomonas DEGRADING OF HYDROCARBONS WITH ITS WILD HOST FISH Chirostoma jordani

Rangel

Madera-Sandoval

Castro-Escarpulli

et al. 2018

Rev. Int. Contam. Ambie.

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The symbiotic relationship between native microbiota and their hosts probably is a key factor in animal survival. In this study, the relationships of Pseudomonas spp. strains with specific biomarkers of exposure to polycyclic aromatic hydrocarbons (PAHs) in wild fish Chirostoma jordani, as well as the capacity of these bacteria to biotransform PAHs were evaluated. The activity of the naphthalene dioxygenase system of the bacteria exposed to 0.1, 1.0 and 10 µg/L of PAHs was higher than the mean isoform 1A1 (CYP1A1) of the wild fish, particularly bacterial species related to Pseudomonas spp. However, the epoxide hydrolase activity of the strains was lower in all cases compared to the fish. Glutathione S-transferase (GST) activity of the bacterial strains was lower than in the liver, but higher than in viscera of C. jordani. Using redundancy analysis, two differential patterns were found: (i) CYP1A1 activity of fish was induced by naphthalene and anthracene water levels independently of sampling season and lakes monitored, and (ii) the unidentified strain of the Pseudomonas genus biotransforms the endogenous levels of benzo[a]pyrene, benzo[k]fluoranthene and indeno[1,2,3-cd]pyrene in the fish allowing the hydroxylated metabolites to conjugate with glutathione through GST activity of the fish. Palabras clave: CYP 1A1, epóxido hidrolasa, GST, HAP, oxidasas, sistema NDO RESUMEN Las relaciones simbióticas entre la microbiota nativa y sus hospederos probablemente son un factor clave en la supervivencia animal. En el presente estudio se evaluaron las relaciones de cepas de Pseudomonas spp. con biomarcadores específicos de exposición a hidrocarburos aromáticos policíclicos (HAP) en Chirostoma jordani silvestre, así como la capacidad de estas bacterias para biotransformar HAP. La actividad del sistema de naftalen-dioxigenasa de las bacterias expuestas a 0,1, 1,0 y 10 μg/L de HAP fue superior al promedio de la isoforma 1A1 (CYP1A1) en los peces, particularmente cepas relacionadas con especies de Pseudomonas spp. Sin embargo, la actividad de la epóxido hidrolasa de las bacterias fue menor en comparación con los peces. La catálisis de la glutatión-S-transferasa (GST) de las cepas bacterianas fue menor que en

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Genome Sequence of Pseudomonas aeruginosa DQ8, an Efficient Degrader of n -Alkanes and Polycyclic Aromatic Hydrocarbons

Cited by 13 publications

References 11 publications

Aerobic bacteria degrading both n-alkanes and aromatic hydrocarbons: an undervalued strategy for metabolic diversity and flexibility

Aerobic bacteria degrading both n-alkanes and aromatic hydrocarbons: an undervalued strategy for metabolic diversity and flexibility

Aerobic biodegradation of organic compounds in hydraulic fracturing fluids

TOXICOKINETIC AND TOXICODYNAMIC SYMBIOTIC INTERACTIONS AMONG INTESTINAL Pseudomonas DEGRADING OF HYDROCARBONS WITH ITS WILD HOST FISH Chirostoma jordani

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