Genome Sequence of Pseudoalteromonas flavipulchra JG1, a Marine Antagonistic Bacterium with Abundant Antimicrobial Metabolites

Yu, Min; Tang, Kaihao; Shi, Xiaochong; Zhang, Xiaohua

doi:10.1128/jb.00598-12

Cited by 10 publications

(4 citation statements)

References 13 publications

(8 reference statements)

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“…A total of 4 913 open reading frames (ORFs) are identified within the P. flavipulchra JG1 genome (Table 1 ). Among the predicted genes, 1 985 (40.4%) are not found in COG categories, and 1 725 (35.1%), 712 (14.5%), 1 863 (37.9%) and 629 (12.8%) genes are not applicable within the KEGG, NR, SwissProt and TrEMBL databases, respectively [ 11 ]. JG1 contains 180 tandem repeat regions, 143 transposons, and 5 ISs (Insert sequences) that account for about 1.1% of the genome.…”

Section: Resultsmentioning

confidence: 99%

Genome analysis of Pseudoalteromonas flavipulchra JG1 reveals various survival advantages in marine environment

Tang

Liu

et al. 2013

BMC Genomics

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BackgroundCompetition between bacteria for habitat and resources is very common in the natural environment and is considered to be a selective force for survival. Many strains of the genus Pseudoalteromonas were confirmed to produce bioactive compounds that provide those advantages over their competitors. In our previous study, P. flavipulchra JG1 was found to synthesize a Pseudoalteromonas flavipulchra antibacterial Protein (PfaP) with L-amino acid oxidase activity and five small chemical compounds, which were the main competitive agents of the strain. In addition, the genome of this bacterium has been previously sequenced as Whole Genome Shotgun project (PMID: 22740664). In this study, more extensive genomic analysis was performed to identify specific genes or gene clusters which related to its competitive feature, and further experiments were carried out to confirm the physiological roles of these genes when competing with other microorganisms in marine environment.ResultsThe antibacterial protein PfaP may also participate in the biosynthesis of 6-bromoindolyl-3-acetic acid, indicating a synergistic effect between the antibacterial macromolecule and small molecules. Chitinases and quorum quenching enzymes present in P. flavipulchra, which coincide with great chitinase and acyl homoserine lactones degrading activities of strain JG1, suggest other potential mechanisms contribute to antibacterial/antifungal activities. Moreover, movability and rapid response mechanisms to phosphorus starvation and other stresses, such as antibiotic, oxidative and heavy metal stress, enable JG1 to adapt to deleterious, fluctuating and oligotrophic marine environments.ConclusionsThe genome of P. flavipulchra JG1 exhibits significant genetic advantages against other microorganisms, encoding antimicrobial agents as well as abilities to adapt to various adverse environments. Genes involved in synthesis of various antimicrobial substances enriches the antagonistic mechanisms of P. flavipulchra JG1 and affords several admissible biocontrol procedures in aquaculture. Furthermore, JG1 also evolves a range of mechanisms adapting the adverse marine environment or multidrug rearing conditions. The analysis of the genome of P. flavipulchra JG1 provides a better understanding of its competitive properties and also an extensive application prospect.

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Section: Resultsmentioning

confidence: 99%

Genome analysis of Pseudoalteromonas flavipulchra JG1 reveals various survival advantages in marine environment

Tang

Liu

et al. 2013

BMC Genomics

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“…anguillarum is inhibited by P . flavipulchra JG1, since it produces bacteriocin PfaP (Yu et al, 2012). Pseudoalteromonas piscicida exerts antibacterial activity against V .…”

Section: Discussionmentioning

confidence: 99%

“…Pseudoalteromonas luteoviolaceae 2ta16 produces 2,4,6 tribromophenol compounds against pathogenic bacteria (Agarwal et al, 2014). Pseudoalteromonas flavipulchra JG1 inhibits the growth of the Gram‐positive and Gram‐negative pathogenic bacteria since it produced 6‐bromoindole‐3 acetic acid compounds (Yu et al, 2012). Prodigiosin, cycloprodigiosin and 2 para h‐benzyl prodigiosin from P .…”

Section: Discussionmentioning

confidence: 99%

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Anti‐ Vibrio activity of Pseudoalteromonas xiamenensis STKMTI.2, a new potential vibriosis biocontrol bacterium in marine aquaculture

Handayani

Isnansetyo

Istiqomah

et al. 2021

Aquaculture Research

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Vibriosis is a prevalent disease in almost all mariculture species worldwide. The pathogens causing this disease can survive freely in water regardless of the host and can very easily infect marine organisms in warm water (Klesius & Pridgeon, 2011). They cause the death of shrimp and marine fish (Istiqomah et al., 2020), which in turn leads to severe economic losses and becomes a major public health problem. These causative bacteria are classified as Gram-negative and opportunistic pathogens that infect the marine organisms when the host is under stress. Clinical signs of infected fish include anorexia, eyes opacity, pigmentation of the fish skin, skin ulcers, lesions in the muscle, exophthalmia, ulcers in the eye, fin rot, and swollen intestines (Mohamad et al., 2019;Zhang et al., 2014).Vibriosis is controlled by administering quinolone, tetracycline and erythromycin. However, the application of antibiotics strengthens the antibiotic-resistant Vibrio. Vibrio spp. have become resistant to antibiotics commonly used for treatment (Isnansetyo et al., 2009). Vibrio campbellii is resistant to oxytetracycline, ampicillin and carbenicillin (Nakayama et al., 2006). It has also been reported that some strains of V. harveyi have become resistant to more than three types of antibi-

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New Report: Genome Mining Untaps the Antibiotics Biosynthetic Gene Cluster of Pseudoalteromonas xiamenensis STKMTI.2 from a Mangrove Soil Sediment

et al. 2022

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Genome Sequence of Pseudoalteromonas flavipulchra JG1, a Marine Antagonistic Bacterium with Abundant Antimicrobial Metabolites

Cited by 10 publications

References 13 publications

Genome analysis of Pseudoalteromonas flavipulchra JG1 reveals various survival advantages in marine environment

Genome analysis of Pseudoalteromonas flavipulchra JG1 reveals various survival advantages in marine environment

Anti‐ Vibrio activity of Pseudoalteromonas xiamenensis STKMTI.2, a new potential vibriosis biocontrol bacterium in marine aquaculture

New Report: Genome Mining Untaps the Antibiotics Biosynthetic Gene Cluster of Pseudoalteromonas xiamenensis STKMTI.2 from a Mangrove Soil Sediment

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