Genome Sequence of Poultry Pathogen
            <i>Riemerella anatipestifer</i>
            Strain RA-YM

Zhou, Zutao; Peng, Xiaoya; Xiao, Yufei; Wang, Xiliang; Guo, Zisheng; Zhu, Lei; Liu, Mei; Jin, Hui; Bi, Dingren; Li, Zili; Sun, Ming

doi:10.1128/jb.01445-10

Cited by 25 publications

(15 citation statements)

References 18 publications

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“…Among the known serotypes of R. anatipestifer, there are huge variations in the virulence between serotypes and between different strains even within the same serotype (Subramaniam et al, 2000). Although four genome sequences of R. anatipestifer of strains ATCC 11845 (Mavromatis et al, 2011), RA-GD (Yuan et al, 2011), RA-YM (Zhou et al, 2010), and RA-SG (Yuan et al, 2013) have been reported, little is known about the molecular basis of the pathogenicity of R. anatipestifer infection, and so far, few virulence factors have been characterized other than virulence-associated protein D (VapD) (Chang et al, 1998), CAMP cohemolysin (Crasta et al, 2002), and outer membrane protein A (OmpA) (Hu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A homolog of glyceraldehyde-3-phosphate dehydrogenase from Riemerella anatipestifer is an extracellular protein and exhibits biological activity

Gao

Zhu

et al. 2014

J. Zhejiang Univ. Sci. B

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Abstract:Riemerella anatipestifer is the causative agent of septicemia anserum exsudativa in ducks. Its pathogenesis and virulence factors are still unclear. The glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), an anchorless and multifunctional protein on the surface of several pathogenic microorganisms, is involved in virulence and adhesion. Whether homologs of GAPDH exist, and display similar characteristics in R. anatipestifer (RaGAPDH) has not been determined. In our research, the RaGAPDH activity from various R. anatipestifer isolates was confirmed. Twenty-two gapdh genes from genomic DNA of R. anatipestifer isolates were cloned and sequenced for phylogenetic analysis. The distribution of RaGAPDH in R. anatipestifer CZ2 strain was confirmed by antisera to recombinant RaGAPDH. The ability of purified RaGAPDH to bind host proteins was analyzed by solid-phase ligandbinding assay. Results revealed that all R. anatipestifer isolates showed different levels of GAPDH activity except four strains, which contained a gapdh-like gene. The gapdh of R. anatipestifer, which is located phylogenetically in the same branch as enterohemorrhagic Escherichia coli (EHEC), belonged to class I GAPDH, and encoded a 36.7-kDa protein. All RaGAPDH-encoding gene sequences from field isolates of R. anatipestifer displayed 100% homology. The RaGAPDH localized on the extracellular membrane of several R. anatipestifer strains. Further, it was released into the culture medium, and exhibited GAPDH enzyme activity. We also confirmed the binding of RaGAPDH to plasminogen and fibrinogen. These results demonstrated that GAPDH was present in R. anatipestifer, although not in all strains, and that RaGAPDH might contribute to the microorganism's virulence.

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Section: Introductionmentioning

confidence: 99%

A homolog of glyceraldehyde-3-phosphate dehydrogenase from Riemerella anatipestifer is an extracellular protein and exhibits biological activity

Gao

Zhu

et al. 2014

J. Zhejiang Univ. Sci. B

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“…Three genomic sequences of R. anatipestifer , published (Yuan et al, 2011) or available as database entries (GenBank accession numbers CP003787 and CP004020), also carry chromosomally encoded genes similar to tet (X) (Figure 1). Interestingly, four other strains of R. anatipestifer , for which genome sequences are available (Mavromatis et al, 2011; Zhou et al, 2011; Wang et al, 2012; Yuan et al, 2013), have not yet acquired tet (X). No information regarding antibiotic use practices at sampling sites where R. anatipestifer strains have been isolated is available in the cited publications.…”

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confidence: 99%

Evolution in action: dissemination of tet(X) into pathogenic microbiota

Aminov¹

2013

Front. Microbiol.

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“…Up to now, at least 21 serotypes have been identified by tube agglutination and agar gel precipitin tests, and no cross-immunoprotection has been reported (5, 6). Based on public papers, three genome sequences of R. anatipestifer have been reported until now, those of strains ATCC 11845, RA-GD, and RA-YM (7–10). All of them are highly virulent to the susceptible hosts.…”

Section: Genome Announcementmentioning

confidence: 99%