Genome Sequence of Pichia kudriavzevii M12, a Potential Producer of Bioethanol and Phytase

Chan, Giek Far; Gan, Han Ming; Ling, How Lie; Rashid, Noor Aini Abdul

doi:10.1128/ec.00229-12

Cited by 63 publications

(52 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although C. krusei , also known as Issatchenkia orientalis or Pichia kudriavzevii , is a relatively poorly studied ascomycetous yeast, it is important for natural food fermentation (Chan et al 2012), especially of cocoa beans for chocolate production. It is also an opportunistic fungal pathogen of humans that has reduced susceptibility to azole antifungals due, in part, to pump-mediated drug efflux (Lamping et al 2009).…”

Section: Resultsmentioning

confidence: 99%

Role of Ectopic Gene Conversion in the Evolution of a Candida krusei Pleiotropic Drug Resistance Transporter Family

et al. 2017

View full text Add to dashboard Cite

Gene duplications enable the evolution of novel gene function, but strong positive selection is required to preserve advantageous mutations in a population. This is because frequent ectopic gene conversions (EGCs) between highly similar, tandem-duplicated, sequences, can rapidly remove fate-determining mutations by replacing them with the neighboring parent gene sequences. Unfortunately, the high sequence similarities between tandem-duplicated genes severely hamper empirical studies of this important evolutionary process, because deciphering their correct sequences is challenging. In this study, we employed the eukaryotic model organism Saccharomyces cerevisiae to clone and functionally characterize all 30 alleles of an important pair of tandem-duplicated multidrug efflux pump genes, ABC1 and ABC11, from seven strains of the diploid pathogenic yeast Candida krusei. Discovery and functional characterization of their closest ancestor, C. krusei ABC12, helped elucidate the evolutionary history of the entire gene family. Our data support the proposal that the pleiotropic drug resistance (PDR) transporters Abc1p and Abc11p have evolved by concerted evolution for ∼134 MY. While >90% of their sequences remained identical, very strong purifying selection protected six short DNA patches encoding just 18 core amino acid (aa) differences in particular trans membrane span (TMS) regions causing two distinct efflux pump functions. A proline-kink change at the bottom of Abc11p TMS3 was possibly fate determining. Our data also enabled the first empirical estimates for key parameters of eukaryotic gene evolution, they provided rare examples of intron loss, and PDR transporter phylogeny confirmed that C. krusei belongs to a novel, yet unnamed, third major Saccharomycotina lineage.

show abstract

Section: Resultsmentioning

confidence: 99%

Role of Ectopic Gene Conversion in the Evolution of a Candida krusei Pleiotropic Drug Resistance Transporter Family

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Digestion of excessive microbial biomass—either associated with food or in the gut itself—may be a major contributor to mammalian nutrition (Kuhad et al, 1997). Interestingly, I. orientalis, also known as Pichia kudriavzevii or Candida krusei (Chan et al, 2012), is commonly associated with several food products, such as wine-grapes (Chavan et al, 2009), cheese (Prillinger et al, 1999), lafun (fermented cassava) (Padonou et al, 2009) and fermented cocoa beans used for chocolate production (Daniel et al, 2009). Food-associated microbes, both bacteria and fungi, are known to colonize the human gut (David et al, 2014), but whether they are able to harvest protein from nutrient-poor diets, similar to I. orientalis , remains to be tested.…”

Section: Discussionmentioning

confidence: 99%

Microbes Promote Amino Acid Harvest to Rescue Undernutrition in Drosophila

et al. 2015

View full text Add to dashboard Cite

Summary Microbes play an important role in the pathogenesis of nutritional disorders such as protein-specific malnutrition. However, the precise contribution of microbes to host energy balance during undernutrition is unclear. Here, we show that Issatchenkia orientalis, a fungal microbe isolated from field-caught Drosophila melanogaster, promotes amino acid harvest to rescue the lifespan of undernourished flies. Using radioisotope-labeled dietary components (amino acids, nucleotides, and sucrose) to quantify nutrient transfer from food to microbe to fly, we demonstrate that I. orientalis extracts amino acids directly from nutrient-poor diets and increases protein flux to the fly. This microbial association restores body mass, protein, glycerol, and ATP levels and phenocopies the metabolic profile of adequately fed flies. Our study uncovers amino acid harvest as a fundamental mechanism linking microbial and host metabolism, and highlights Drosophila as a platform for quantitative studies of host-microbe relationships.

show abstract

“…This yeast has a biotechnological potential for bio-ethanol (Dhaliwal et al 2011) production, in agriculture practices as bio fertilizer (Chan et al 2012) or in the bioremediation of heavy metals (Ubeda et al 2014). The efficiency of heavy metal bioremediation by a metabolism-dependent process is influenced by the impact of the heavy metals on yeast cells.…”

Section: Discussionmentioning

confidence: 99%

Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii

Mesquita

Machado

Silva

et al. 2015

Environ Sci Pollut Res

View full text Add to dashboard Cite

Metal contamination of the environment is frequently associated to the presence of two or more metals. This work aimed to study the impact of a mixture of metals (Cd, Pb and Zn) on the physiology of the non-conventional yeast Pichia kudriavzevii. The incubation of yeast cells with 5 mg/ l Cd, 10 mg/l Pb and 5 mg/l Zn, for 6 h, induced a loss of metabolic activity (assessed by FUN-1 staining) and proliferation capacity (evaluated by a clonogenic assay), with a small loss of membrane integrity (measured by trypan blue exclusion assay). The staining of yeast cells with calcofluor white revealed that no modification of chitin deposition pattern occurred during the exposure to metal mixture. Extending for 24 h, the exposure of yeast cells to metal mixture provoked a loss of membrane integrity, which was accompanied by the leakage of intracellular components. A marked loss of the metabolic activity and the loss of proliferation capacity were also observed. The analysis of the impact of a single metal has shown that, under the conditions studied, Pb was the metal responsible for the toxic effect observed in the metal mixture. Intracellular accumulation of Pb seems to be correlated with the metals' toxic effects observed.

show abstract

Genome Sequence of Pichia kudriavzevii M12, a Potential Producer of Bioethanol and Phytase

Cited by 63 publications

References 19 publications

Role of Ectopic Gene Conversion in the Evolution of a Candida krusei Pleiotropic Drug Resistance Transporter Family

Role of Ectopic Gene Conversion in the Evolution of a Candida krusei Pleiotropic Drug Resistance Transporter Family

Microbes Promote Amino Acid Harvest to Rescue Undernutrition in Drosophila

Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii

Contact Info

Product

Resources

About