Genome Sequence of Non-O1 Vibrio cholerae PS15

Kumar, Sanath; Lindquist, Ingrid; Sundararajan, Anitha; Rajanna, Chythanya; Floyd, Jared T.; Smith, Kenneth P.; Andersen, Jody L.; He, Gui-Xin; Ayers, Ryan M.; Johnson, Judith A.; Werdann, James J.; Sandoval, Ava A.; Mojica, Nadia M.; Schilkey, Faye; Mudge, Joann; Varela, Manuel F.

doi:10.1128/genomea.00227-12

Cited by 14 publications

(17 citation statements)

References 11 publications

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“…Towards these possibilities, an assortment of approaches have been tried. One of the most promising of these methods involves comparative genomics [175][176][177]. Comparing the whole genomes of known pathogens with those of strains from closely-related non-pathogenic counterparts can reveal key genetic elements that are indispensable for the growth and survival of pathogens and is a consistently promising approach.…”

Section: Discussionmentioning

confidence: 99%

Functional and Structural Roles of the Major Facilitator Superfamily Bacterial Multidrug Efflux Pumps

et al. 2020

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Pathogenic microorganisms that are multidrug-resistant can pose severe clinical and public health concerns. In particular, bacterial multidrug efflux transporters of the major facilitator superfamily constitute a notable group of drug resistance mechanisms primarily because multidrug-resistant pathogens can become refractory to antimicrobial agents, thus resulting in potentially untreatable bacterial infections. The major facilitator superfamily is composed of thousands of solute transporters that are related in terms of their phylogenetic relationships, primary amino acid sequences, two- and three-dimensional structures, modes of energization (passive and secondary active), and in their mechanisms of solute and ion translocation across the membrane. The major facilitator superfamily is also composed of numerous families and sub-families of homologous transporters that are conserved across all living taxa, from bacteria to humans. Members of this superfamily share several classes of highly conserved amino acid sequence motifs that play essential mechanistic roles during transport. The structural and functional importance of multidrug efflux pumps that belong to the major facilitator family and that are harbored by Gram-negative and -positive bacterial pathogens are considered here.

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Section: Discussionmentioning

confidence: 99%

Functional and Structural Roles of the Major Facilitator Superfamily Bacterial Multidrug Efflux Pumps

et al. 2020

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“…The emrD-3 gene was obtained from V. cholerae O395, an O1 serotype strain that was a kind gift from Dr. Chythanya Rajanna (Emerging Pathogens Institute, Gainesville, FL) (Mekalanos 1983). V. cholerae strains N16961 (O1 El tor) and PS15 (non-O1, isolate from Puget Sound, WA) were from Heidelberg et al (Heidelberg et al 2000) and courtesy of Dr. Charles Kaysner (Kumar et al 2013c;Mukherjee et al 2014), respectively. E. coli KAM32, which was a generous gift from Professor Tomofusa Tsuchiya (Okayama University, Japan), was the host cell for the emrD-3 gene (Chen et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Modulation of the multidrug efflux pump EmrD-3 from Vibrio cholerae by Allium sativum extract and the bioactive agent allyl sulfide plus synergistic enhancement of antimicrobial susceptibility by A. sativum extract

et al. 2017

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The causative agent of cholera, Vibrio cholerae, is a public health concern. Multidrug-resistant V. cholerae variants may reduce chemotherapeutic efficacies of severe cholera. We previously reported that the multidrug efflux pump EmrD-3 from V. cholerae confers resistance to multiple structurally distinct antimicrobials. Medicinal plant compounds are potential candidates for EmrD-3 efflux pump modulation. The antibacterial activities of garlic Allium sativum, although poorly understood, predicts that a main bioactive component, allyl sulfide, modulates EmrD-3 efflux. Thus, we tested whether A. sativum extract acts in synergy with antimicrobials and that a main bioactive component allyl sulfide inhibits EmrD-3 efflux. We found that A. sativum extract and allyl sulfide inhibited ethidium bromide efflux in cells harboring EmrD-3 and that A. sativum lowered the MICs of multiple antibacterials. We conclude that A. sativum and allyl sulfide inhibit EmrD-3 and that A. sativum extract synergistically enhances antibacterial agents.

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“…The non-O1, non-O139 strain HE-09 isolated from the environment in Haiti (11, 37) and strain PS-15 isolated from Puget Sound in Washington State (65) also have unique toxin structures. For both strains, the toxins have in the first position, DUF1, a domain of unknown function that has no homology to any non-MARTX proteins.…”

Section: Martx Toxins Of V Choleraementioning

confidence: 99%

Multifunctional-autoprocessing repeats-in-toxin (MARTX) Toxins of Vibrios

Satchell

2015

Microbiol Spectr

105

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Multifunctional-autoprocessing repeats-in-toxin (MARTX) toxins are a heterogeneous group of toxins found in a number of Vibrio species and other Gram-negative bacteria. The toxins are composed of conserved repeat regions and an autoprocessing protease domain that together function as a delivery platform for transfer of cytotoxic and cytopathic domains into target eukaryotic cell cytosol. Within the cells, the effectors can alter biological processes such as signaling or cytoskeletal structure, presumably to the benefit of the bacterium. Ten effector domains are found in the various Vibrio MARTX toxins, although any one toxin carries only two to five effector domains. The specific toxin variant expressed by a species can be modified by homologous recombination to acquire or lose effector domains, such that different strains within the same species can express distinct variants of the toxins. This review examines the conserved structural elements of the MARTX toxins and details the different toxin arrangements carried by Vibrio species and strains. The catalytic function of domains and how the toxins are linked to pathogenesis of human and animals is described.

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Genome Sequence of Non-O1 Vibrio cholerae PS15

Abstract: The draft genome sequence of a non-O1 Vibrio cholerae strain, PS15, organized into 3,512 open reading frames within a 3.9-Mb genome, was determined. The PS15 genome sequence will allow for the study of the evolution of virulence and environmental adaptation in V. cholerae.

Cited by 14 publications

References 11 publications

Functional and Structural Roles of the Major Facilitator Superfamily Bacterial Multidrug Efflux Pumps

Functional and Structural Roles of the Major Facilitator Superfamily Bacterial Multidrug Efflux Pumps

Modulation of the multidrug efflux pump EmrD-3 from Vibrio cholerae by Allium sativum extract and the bioactive agent allyl sulfide plus synergistic enhancement of antimicrobial susceptibility by A. sativum extract

Multifunctional-autoprocessing repeats-in-toxin (MARTX) Toxins of Vibrios

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