Genome Sequence of Litorilinea aerophila, an Icelandic Intertidal Hot Springs Bacterium

Maurais, Elizabeth G; Iannazzi, Lauren C; MacLea, Kyle S.

doi:10.1128/mra.01206-21

Cited by 2 publications

(2 citation statements)

References 24 publications

(30 reference statements)

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“…7 ). Investigation on the 45 Chloroflexota genomes having FlaB (found in AnnoTree) and BlastP searches at NCBI (FlaB of strain JH545 as a query) revealed that at least two Chloroflexota strains harboring archaella gene cluster (flaB and several other genes) have been cultivated: Litorilinea aerophila 30 , 31 and Aggregatilinea lenta 32 (Supplementary Fig. 6 ).…”

Section: Resultsmentioning

confidence: 99%

Cultivation of marine bacteria of the SAR202 clade

Lim,

Seo,

Giovannoni

et al. 2023

Nat Commun

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Bacteria of the SAR202 clade, within the phylum Chloroflexota, are ubiquitously distributed in the ocean but have not yet been cultivated in the lab. It has been proposed that ancient expansions of catabolic enzyme paralogs broadened the spectrum of organic compounds that SAR202 bacteria could oxidize, leading to transformations of the Earth’s carbon cycle. Here, we report the successful cultivation of SAR202 bacteria from surface seawater using dilution-to-extinction culturing. The growth of these strains is very slow (0.18–0.24 day−1) and is inhibited by exposure to light. The genomes, of ca. 3.08 Mbp, encode archaella (archaeal motility structures) and multiple sets of enzyme paralogs, including 80 genes coding for enolase superfamily enzymes and 44 genes encoding NAD(P)-dependent dehydrogenases. We propose that these enzyme paralogs participate in multiple parallel pathways for non-phosphorylative catabolism of sugars and sugar acids. Indeed, we demonstrate that SAR202 strains can utilize several substrates that are metabolized through the predicted pathways, such as sugars ʟ-fucose and ʟ-rhamnose, as well as their lactone and acid forms.

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Section: Resultsmentioning

confidence: 99%

Cultivation of marine bacteria of the SAR202 clade

Lim,

Seo,

Giovannoni

et al. 2023

Nat Commun

View full text Add to dashboard Cite

show abstract

“…This distribution suggests that archaella genes might have been transferred from Archaea to an ancestor of Chloroflexota and retained in the class Dehalococcoidia. Investigation on the 45 Chloroflexota genomes having FlaB (found in AnnoTree) and BlastP searches at NCBI (FlaB of strain JH545 as a query) revealed that at least two Chloroflexota strains harboring archaella gene cluster (flaB and several other genes) have been cultivated: Litorilinea aerophila 30,31 and Aggregatilinea lenta 32 (Extended Data Fig. 5).…”

Section: Genomic Featuresmentioning

confidence: 99%

Cultivation of SAR202 Bacteria from the Ocean

Seo

Giovannoni

et al. 2023

Preprint

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Here we report the first successful cultivation of SAR202 bacteria, a superorder in the phylum Chloroflexota, which have long been at the top of "most wanted" lists of uncultivated microbial life. It has been proposed that ancient expansions of catabolic enzyme paralogs in SAR202 broadened the spectrum of organic compounds they could oxidize, leading to transformations of the Earth's carbon cycle. We cultured the cells from surface seawater using dilution-to-extinction culturing. Their growth was very slow (0.18-0.24 day-1) and was inhibited by exposure to light. The genomes, of ca. 3.08 Mbp, encoded archaella, archaeal motility structures, and multiple sets of paralogs, including 80 genes in enolase superfamily and 44 genes in NAD(P)-dependent dehydrogenase family. We propose that these paralogs participate in multiple parallel pathways of non-phosphorylative sugar and sugar acid catabolism, and demonstrate that, as predicted by this scheme, the sugars L-fucose and L-rhamnose and their lactone and acid forms are utilized by these cells.

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Genome Sequence of Litorilinea aerophila, an Icelandic Intertidal Hot Springs Bacterium

Cited by 2 publications

References 24 publications

Cultivation of marine bacteria of the SAR202 clade

Cultivation of marine bacteria of the SAR202 clade

Cultivation of SAR202 Bacteria from the Ocean

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