Genome Sequence of Listeria monocytogenes 07PF0776, a Cardiotropic Serovar 4b Strain

McMullen, Phillip; Gillaspy, Allison F.; Gipson, Jenny; Bobo, Linda; Skiest, Daniel J.; Freitag, Nancy E.

doi:10.1128/jb.00616-12

Cited by 12 publications

(4 citation statements)

References 9 publications

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“…On the other hand, we had high serologies for serotypes 1/2a and 4b, strongly suggestive of infection. Additionally, listeria subpopulations with cardiotropic activity have been shown [14,15].…”

Section: Discussionmentioning

confidence: 99%

A Rare Case of Pericarditis due to Listeria monocytogenes 1/2a and 4b Serotypes

Domingues¹,

Aroso²,

Gonçalves³

et al. 2019

J Med Cases

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Listeriosis is associated with the consumption of contaminated food and has a higher incidence in the elderly, immunocompromised and pregnant. There are a few reported cases of pericarditis due to Listeria monocytogenes, with a high mortality. We present a successful case of Listeria monocytogenes pericarditis in a 61-year-old patient and provide the clinical presentation, pathology and diagnostic challenge. Increasing awareness of foodborne infections would be important to promote its early diagnosis.

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Section: Discussionmentioning

confidence: 99%

A Rare Case of Pericarditis due to Listeria monocytogenes 1/2a and 4b Serotypes

Domingues¹,

Aroso²,

Gonçalves³

et al. 2019

J Med Cases

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“…To establish the relevance of our observations in strain 10403s, we also investigated the effects of SCFA exposure on LLO production in the cardiotropic strain 07PF0776 (serotype 4b) [31] to identify any strain-dependent responses to SCFAs. Strain 07PF0776 in general exhibited lower supernatant LLO activities under aerobic conditions ( Figure 4A) but higher LLO activities under anaerobic conditions ( Figure 4B) than strain 10403s.…”

Section: Production Of Llo In Strain 07pf0776 Was Sensitive To Regulamentioning

confidence: 99%

“…To determine whether the ability to sense and respond to SCFAs is a relevant phenotype for common virulent strains of L. monocytogenes, we compared the effects of SCFAs between the lab strain 10403s, serotype 1/2a, and strain 07PF0776, a cardiotropic strain of L. monocytogenes belonging to serotype 4b [31,59]. Direct comparisons between these two strains have demonstrated that strain 07PF0776 established a significantly higher bacterial burden in the hearts of female ND4 Swiss Webster mice than strain 10403s after intravenous infection [59].…”

Section: Strain-dependent Variations In Scfa Responsesmentioning

confidence: 99%

“…To quantify hly transcription, an hly transcriptional reporter strain (Phly-gus-neo, NF-L695, provided by Dr. Nancy Freitag at The University of Illinois College of Medicine) with the hly promoter driving the expression of β-glucuronidase (GUS) [83] was used. A cardiotropic strain 07PF0776 (provided by Dr. Nancy Freitag at The University of Illinois College of Medicine) [31] was also used in this study. Bacteria were streaked weekly on brain heart infusion (BHI) agar to provide fresh colonies for experiments.…”

Section: Strainsmentioning

confidence: 99%

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The Production of Listeriolysin O and Subsequent Intracellular Infections by Listeria monocytogenes Are Regulated by Exogenous Short Chain Fatty Acid Mixtures

Rinehart

Chapman

Sun

2020

Toxins

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Listeria monocytogenes is a foodborne pathogen capable of secreting listeriolysin O (LLO), a pore-forming toxin encoded by the hly gene. While the functions of LLO have been studied extensively, how the production of LLO is modulated by the intestinal environment, devoid of oxygen and enriched in short chain fatty acids (SCFAs), is not completely understood. Using L. monocytogenes strain 10403s, we found that hly transcription was moderately decreased by aerobic SCFA exposures but significantly increased by anaerobic SCFA exposures. Moreover, aerobic, but not anaerobic, exposure to low levels of SCFAs resulted in a significantly higher LLO activity. These results demonstrated that transcriptional and post-transcriptional regulations of LLO production were separately modulated by SCFAs and were responsive to oxygen levels. Examining isogenic mutants revealed that PrfA and SigB play a role in regulating LLO production in response to SCFAs. Effects of SCFAs were also present in the cardiotropic strain 07PF0776 but distinctly different from those in strain 10403s. For both strains, prior exposures to SCFAs altered intracellular infections in Caco-2 and RAW264.7 cells and the plaque sizes in L fibroblasts, a result confirming the ability of L. monocytogenes to adapt to SCFAs in ways that impact its subsequent infection outcomes.

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Mining of novel species-specific primers for PCR detection of Listeria monocytogenes based on genomic approach

Tao

Chen

Bie

et al. 2015

World J Microbiol Biotechnol

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Listeria monocytogenes in contaminated food is considered as a serious health threat for consumers due to its high mortality rate. The objective of this study was to obtain novel species-specific target-genes and primers for the molecular detection of L. monocytogenes using a comparative genomic approach. By comparative analysis of L. monocytogenes and non-L. monocytogenes genome sequences in the GenBank database with BLAST program, 26 specific target sequences were used as candidates and the primers were designed for L. monocytogenes species-specificity verification by using PCR assay. Finally, the three genes LMOf2365_0970, LMOf2365_2721 and mpl were identified to have L. monocytogenes species-specificity and be unique as detection targets for diagnostic application. The species-specific primer Lm8 of gene LMOf2365_0970, Lm13 of gene LMOf2365_2721 and Lm20 of gene mpl showed better specificity and sensitivity than the primers described previously. The PCR detection limits of the three specific primer sets were 430, 43, 4.3 fg/μL for genomic DNA, and 5 × 10(3), 50, 5 cfu/mL for pure culture of L. monocytogenes. There was no interference in specificity of detecting L. monocytogenes by co-culture with other foodborne pathogens in high concentration. Moreover, after 6-8 h of enrichment, L. monocytogenes in the artificially contaminated milk samples at an inoculum dose of 38 cfu/10 mL milk could be detected successfully with the studied three primers. Therefore, the three specific genes and primers can be applied to establish a novel rapid and accurate method for detecting L. monocytogenes in food materials.

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Genome Sequence of Listeria monocytogenes 07PF0776, a Cardiotropic Serovar 4b Strain

Cited by 12 publications

References 9 publications

A Rare Case of Pericarditis due to Listeria monocytogenes 1/2a and 4b Serotypes

A Rare Case of Pericarditis due to Listeria monocytogenes 1/2a and 4b Serotypes

The Production of Listeriolysin O and Subsequent Intracellular Infections by Listeria monocytogenes Are Regulated by Exogenous Short Chain Fatty Acid Mixtures

Mining of novel species-specific primers for PCR detection of Listeria monocytogenes based on genomic approach

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