Genome Sequence of Lactobacillus fermentum Strain NCC2970 (CNCM I-5068)

Barretto, Caroline; Ngom-Bru, Catherine; Genevaz, Alienor; Fournier, Coralie; Moine, D; Kassam, Mohamed; Iltis, Agnès; Sagory-Zalkind, Pierre; Ciron, Pierre-Emmanuel; Faucherand, Gilles; Descombes, Patrick; Duboux, Stéphane

doi:10.1128/genomea.01254-16

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“…Even though MS-MS could not assign a unique sequence to the peptide responsible for the excellent antiproliferative activity shown by SPFs deriving from LAC92, it was possible to confirm the amino acid structure involved in biological activity, thanks to the associated bacterial genomic sequence. These kinds of peptides are typically involved in the PG of L. fermentum ( Barretto et al, 2016 ) . So far, L. casei is the strain that has mostly been proven to have immunomodulatory and antitumor effects ( Lee et al, 2004 ; Jacouton et al, 2018 ; Kim et al, 2019 ; Dadfarma et al, 2021 ; Spyridopoulou et al, 2021 ), but also live L. fermentum strain exerted the ability to inhibit cancer cells but not normal colon cells and protected them from the damaging effect of a carcinogen ( Kahouli et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Soluble peptidoglycan fragments produced by Limosilactobacillus fermentum with antiproliferative activity are suitable for potential therapeutic development: A preliminary report

Fuochi

Spampinato²,

Distefano³

et al. 2023

Front. Mol. Biosci.

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Currently, the use of probiotic strains and their products represents a promising innovative approach as an antagonist treatment against many human diseases. Previous studies showed that a strain of Limosilactobacillus fermentum (LAC92), previously defined as Lactobacillus fermentum, exhibited a suitable amensalistic property. The present study aimed to purify the active components from LAC92 to evaluate the biological properties of soluble peptidoglycan fragments (SPFs). The cell-free supernatant (CFS) and bacterial cells were separated after 48 h of growth in MRS medium broth and treated for isolation of SPFs. Antimicrobial activity and proliferation analysis on the human cell line HTC116 were performed using technologies such as xCELLigence, count and viability, and clonogenic analysis. MALDI-MS investigation and docking analysis were performed to determine the molecular structure and hypothetical mode of action, respectively. Our results showed that the antimicrobial activity was mainly due to SPFs. Moreover, the results obtained when investigating the SPF effect on the cell line HCT116 showed substantial preliminary evidence, suggesting their significant cytostatic and quite antiproliferative properties. Although MALDI was unable to identify the molecular structure, it was subsequently revealed by analysis of the bacterial genome. The amino acid structure is called peptide 92. Furthermore, we confirmed by molecular docking studies the interaction of peptide 92 with MDM2 protein, the negative regulator of p53. This study showed that SPFs from the LAC92 strain exerted anticancer effects on the human colon cancer HCT116 cell line via antiproliferation and inducing apoptosis. These findings indicated that this probiotic strain might be a potential candidate for applications in functional products in the future. Further examination is needed to understand the specific advantages of this probiotic strain and improve its functional features to confirm these data. Moreover, deeper research on peptide 92 could increase our knowledge and help us understand if it will be possible to apply to specific diseases such as CRC.

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