Genome Sequence of Klebsiella pneumoniae YBQ, a Clinical Strain Isolated from the Sputum of a Patient with Severe Pneumonia

Zou, Jintao; Chen, Zhi-Fu; Zhang, Xiaoli; Yuan, Yue; Jing, Huaiqi; Zou, Quanming; Zhang, Jinyong

doi:10.1128/mra.01223-20

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“…Klebsiella pneumoniae strain YBQ was newly isolated from the sputum of a patient with severe pneumonia, and its genome was sequenced [ 24 ]. CPS genotyping by PCR detection of serotype-specific alleles at wzy and wzx loci [ 33 ] showed that YBQ belongs to neither K1 nor K2 serotypes.…”

Section: Discussionmentioning

confidence: 99%

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Host Immune Response to Clinical Hypervirulent Klebsiella pneumoniae Pulmonary Infections via Transcriptome Analysis

Lei

Zhang

Yang

et al. 2022

Journal of Immunology Research

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Klebsiella pneumoniae (K. pneumoniae), especially those with hypervirulence, is becoming a global concern and posing great threat to human health. Studies on individual immune cells or cytokines have partially revealed the function of the host immune defense against K. pneumoniae pulmonary infection. However, systematic immune response against K. pneumoniae has not been fully elucidated. Herein, we report a transcriptome analysis of the lungs from a mouse pneumonia model infected with a newly isolated K. pneumoniae clinical strain YBQ. Total RNA was isolated from the lungs of mice 48 hours post infection to assess transcriptional alteration of genes. Transcriptome data were analyzed with KEGG, GO, and ICEPOP. Results indicated that upregulated transcription level of numerous cytokines and chemokines was coordinated with remarkably activated ribosome and several critical immune signaling pathways, including IL-17 and TNF signaling pathways. Notably, transcription of cysteine cathepsin inhibitor (stfa1, stfa2, and stfa3) and potential cysteine-type endopeptidase inhibitor (cstdc4, cstdc5, and cstdc6) were upregulated. Results of ICEPOP showed neutrophils functions as the most essential cell type against K. pneumoniae infection. Critical gene alterations were further validated by rt-PCR. Our findings provided a global transcriptional perspective on the mechanisms of host defense against K. pneumoniae infection and revealed some unique responding genes.

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Section: Discussionmentioning

confidence: 99%

“…2011–04). K. pneumoniae strain YBQ was isolated from the sputum of a patient with severe pneumonia [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Host Immune Response to Clinical Hypervirulent Klebsiella pneumoniae Pulmonary Infections via Transcriptome Analysis

Lei

Zhang

Yang

et al. 2022

Journal of Immunology Research

Self Cite

View full text Add to dashboard Cite

show abstract

Genome Sequence of Klebsiella pneumoniae YBQ, a Clinical Strain Isolated from the Sputum of a Patient with Severe Pneumonia

Cited by 1 publication

References 19 publications

Host Immune Response to Clinical Hypervirulent Klebsiella pneumoniae Pulmonary Infections via Transcriptome Analysis

Host Immune Response to Clinical Hypervirulent Klebsiella pneumoniae Pulmonary Infections via Transcriptome Analysis

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