Genome sequence of Isaria javanica and comparative genome analysis insights into family S53 peptidase evolution in fungal entomopathogens

Lin, Runmao; Zhang, Xi; Xin, Bei; Zou, Manling; Yaoyao, Gao; Qin, F. Xiao-Feng; Hu, Qiongbo; Xie, Bingyan; Cheng, Xinyue

doi:10.1007/s00253-019-09997-4

Cited by 15 publications

(18 citation statements)

References 81 publications

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“…Ant-infecting Ophiocordyceps genomes are enriched for heat-labile enterotoxins compared to generalist fungal pathogens and have been suggested to play a major role in Ophiocordyceps pathogenesis. For example, O. kimflemingiae has 36 putative enterotoxins, Ophiocordyceps australis has 20, and Ophiocordyceps polyrhachis-furcata has 22 ( Wichadakul et al 2015 ; de Bekker et al 2017a ), while the generalist entomopathogens C. bassiana , M. robertsii , and Isaria javanica have 13, six, and five enterotoxins, respectively ( Xiao et al 2012 ; Mannino et al 2019 ; Lin et al 2019 ). The number of putative enterotoxin genes, their notable upregulation, and membership in manipulation associated gene modules strongly suggest a role for these toxins during Ophiocordyceps infection and manipulation ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

“…However, the highly species-specific ant manipulating Ophiocordyceps carry fewer subtilases (O. camponoti-floridani n = 16, O. kimflemingiae n = 18, and other Ophiocordyceps species n < 20) compared to generalist entomopathogenic fungi ( C. bassiana n = 43, Cordyceps militaris n = 26, M. anisopliae n = 55, and Metarhizium acridum n = 43) ( Gao et al 2011 ; Zheng et al 2011 ; Xiao et al 2012 ; de Bekker et al 2015 ; Wichadakul et al 2015 ). A larger repertoire of proteolytic enzymes may facilitate infection of various host species and is thought to be associated with a broader host range in fungal entomopathogens ( Xiao et al 2012 ; Lin et al 2019 ). The relatively low number of subtilases in Ophiocordyceps genomes corroborates their high species-specificity ( de Bekker et al 2014b ; Araújo et al 2018 ; Sakolrak et al 2018 ).…”

Section: Resultsmentioning

confidence: 99%

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Genetic Underpinnings of Host Manipulation byOphiocordycepsas Revealed by Comparative Transcriptomics

Will

Das

Trinh

et al. 2020

G3 Genes|Genomes|Genetics

234

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Ant-infecting Ophiocordyceps fungi are globally distributed, host manipulating, specialist parasites that drive aberrant behaviors in infected ants, at a lethal cost to the host. An apparent increase in activity and wandering behaviors precedes a final summiting and biting behavior onto vegetation, which positions the manipulated ant in a site beneficial for fungal growth and transmission. We investigated the genetic underpinnings of host manipulation by: (i) producing a high-quality hybrid assembly and annotation of the Ophiocordyceps camponoti-floridani genome, (ii) conducting laboratory infections coupled with RNAseq of O. camponoti-floridani and its host, Camponotus floridanus, and (iii) comparing these data to RNAseq data of Ophiocordyceps kimflemingiae and Camponotus castaneus as a powerful method to identify gene expression patterns that suggest shared behavioral manipulation mechanisms across Ophiocordyceps-ant species interactions. We propose differentially expressed genes tied to ant neurobiology, odor response, circadian rhythms, and foraging behavior may result by activity of putative fungal effectors such as enterotoxins, aflatrem, and mechanisms disrupting feeding behaviors in the ant.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genetic Underpinnings of Host Manipulation byOphiocordycepsas Revealed by Comparative Transcriptomics

Will

Das

Trinh

et al. 2020

G3 Genes|Genomes|Genetics

234

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“…For group two, the following sequences were obtained from GenBank: RKF81808 [ 66 ], XP_012049996 [ 67 ], AFR95641 [ 67 ], KIY70151 [ 68 ], and CCU75063 [ 69 ]. The following protein sequences from GenBank were placed in group three: EKD13246 [ 70 ], KAF7574864 [ 65 ], KAF7566347 [ 65 ], KAF5700252 [ 71 ], KAF4778018 [ 72 ], TQW08525 [ 73 ], RFU72965 [ 74 ], XP_018180561 [ 75 ], OHW96986 [ 76 ], KZL65578 [ 77 ], and XP_007290459 [ 70 ].…”

Section: Methodsmentioning

confidence: 99%

Virulence Traits and Population Genomics of the Black Yeast Aureobasidium melanogenum

Černoša

Sun²,

Fang³

et al. 2021

JoF

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The black yeast-like fungus Aureobasidium melanogenum is an opportunistic human pathogen frequently found indoors. Its traits, potentially linked to pathogenesis, have never been systematically studied. Here, we examine 49 A. melanogenum strains for growth at 37 °C, siderophore production, hemolytic activity, and assimilation of hydrocarbons and human neurotransmitters and report within-species variability. All but one strain grew at 37 °C. All strains produced siderophores and showed some hemolytic activity. The largest differences between strains were observed in the assimilation of hydrocarbons and human neurotransmitters. We show for the first time that fungi from the order Dothideales can assimilate aromatic hydrocarbons. To explain the background, we sequenced the genomes of all 49 strains and identified genes putatively involved in siderophore production and hemolysis. Genomic analysis revealed a fairly structured population of A. melanogenum, raising the possibility that some phylogenetic lineages have higher virulence potential than others. Population genomics indicated that the species is strictly clonal, although more than half of the genomes were diploid. The existence of relatively heterozygous diploids in an otherwise clonal species is described for only the second time in fungi. The genomic and phenotypic data from this study should help to resolve the non-trivial taxonomy of the genus Aureobasidium and reduce the medical hazards of exploiting the biotechnological potential of other, non-pathogenic species of this genus.

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“…PFAMs associated with genes that have possible roles in manipulation include: Heat-labile enterotoxin alpha chain, LysM domain, Peptidase inhibitor I9, and Peptidase M43 (indicated by black dots). LIN & al. 2019, MANNINO & al.…”

Section: Ophiocordyceps Upregulate Adp-ribosylating Toxins and Ribotomentioning

confidence: 99%

“…Expansion of protease gene copies has been suggested to be associated with a broader host range in fungal entomopathogens as a large repertoire of proteolytic enzymes may facilitate infection of various host species (XIAO & al. 2012, LIN & al. 2019.…”

Section: Fungal Serine Proteases Are Upregulated During Manipulated Bmentioning

confidence: 99%

Genetic underpinnings of host manipulation byOphiocordycepsas revealed by comparative transcriptomics

Will

Das

Trinh

et al. 2020

Preprint

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AbstractThe ant-infecting Ophiocordyceps fungi are globally distributed, host manipulating, specialist parasites that drive aberrant behaviors in infected ants, at a lethal cost to the host. An apparent increase in activity and wandering behaviors precedes a final summiting and biting behavior on to vegetation, positioning the manipulated ant in a site beneficial for fungal growth and transmission. Notably, across Ophiocordyceps species and other known host manipulators, the molecular mechanisms underlying behavioral changes remain largely unclear. We explored possible genetic underpinnings of host manipulation by: (i) producing a hybrid assembly of the Ophiocordyceps camponoti-floridani genome, (ii) conducting laboratory infections coupled with RNAseq of both O. camponoti-floridani and its host, Campontous floridanus, and (iii) using these data for a comparative analysis to similar work performed in Ophiocordyceps kimflemingiae and Camponotus castaneus. We propose differentially expressed genes tied to ant neurobiology, odor response, circadian rhythms, and foraging behavior may be the result of putative fungal effectors such as enterotoxins, aflatrem, and mechanisms disrupting nutrition-sensing or caste-identity pathways.

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Genome sequence of Isaria javanica and comparative genome analysis insights into family S53 peptidase evolution in fungal entomopathogens

Cited by 15 publications

References 81 publications

Genetic Underpinnings of Host Manipulation byOphiocordycepsas Revealed by Comparative Transcriptomics

Genetic Underpinnings of Host Manipulation byOphiocordycepsas Revealed by Comparative Transcriptomics

Virulence Traits and Population Genomics of the Black Yeast Aureobasidium melanogenum

Genetic underpinnings of host manipulation byOphiocordycepsas revealed by comparative transcriptomics

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