Genome Sequence of
            <i>Pseudomonas</i>
            sp. Strain P482, a Tomato Rhizosphere Isolate with Broad-Spectrum Antimicrobial Activity

Krzyżanowska, D.; Ossowicki, Adam; Jafra, Sylwia

doi:10.1128/genomea.00394-14

Cited by 15 publications

(17 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strain SVBP6 is the third reported and sequenced isolate of the recently described species P. donghuensis (Gao et al, 2012;Krzyzanowska et al, 2014;Agaras et al, 2018). The first isolate and type-strain HYS was found in the water of the Chinese lake Donghu and it has been characterized as a potent producer of siderophores (Gao et al, 2015), whereas the second isolate P482 was recovered from the rhizosphere of tomato plants in Poland and it showed biocontrol activity against soft-rotting bacteria (Krzyzanowska et al, 2014). The genome sequence of the three strains shares a high degree of similarity, with ANI values over 99.5% (Agaras et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Every predicted protein within this 16-kb region shows a level of identity of at least 99% with proteins encoded by the genomes of the two other published P. donghuensis strains, HYS and P482 (Gao et al, 2012;Krzyzanowska et al, 2014). Outside the Pseudomonas genus, the putative operon orf4-7 encodes proteins with highly identical orthologs (75%-78%) present in members of the proteobacterial species Stenotrophomonas rhizophila and Collimonas sp.…”

Section: Identification Of a Gene Cluster Involved In Fungal Antagonismmentioning

confidence: 98%

See 1 more Smart Citation

7‐hydroxytropolone is the main metabolite responsible for the fungal antagonism of Pseudomonas donghuensis strain SVBP6

Muzio

Agaras

Masi

et al. 2020

Environmental Microbiology

View full text Add to dashboard Cite

Summary Pseudomonas donghuensis strain SVBP6, an isolate from an agricultural plot in Argentina, displays a broad‐spectrum and diffusible antifungal activity, which requires a functional gacS gene but could not be ascribed yet to known secondary metabolites typical of Pseudomonas biocontrol species. Here, we report that Tn5 mutagenesis allowed the identification of a gene cluster involved in both the fungal antagonism and the production of a soluble tropolonoid compound. The ethyl acetate extract from culture supernatant showed a dose‐dependent inhibitory effect against the phytopathogenic fungus Macrophomina phaseolina. The main compound present in the organic extract was identified by spectroscopic and X‐ray analyses as 7‐hydroxytropolone (7HT). Its structure and tautomerism was confirmed by preparing the two key derivatives 2,3‐dimethoxy‐ and 2,7‐dimethoxy‐tropone. 7HT, but not 2,3‐ or 2,7‐dimethoxy‐tropone, mimicked the fungal inhibitory activity of the ethyl acetate extract from culture supernatant. The activity of 7HT, as well as its production, was barely affected by the presence of up to 50 μM added iron (Fe+2). To summarize, P. donghuensis SVBP6 produces 7HT under the positive control of the Gac‐Rsm cascade and is the main active metabolite responsible for the broad‐spectrum inhibition of different phytopathogenic fungi.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Identification Of a Gene Cluster Involved In Fungal Antagonismmentioning

confidence: 98%

7‐hydroxytropolone is the main metabolite responsible for the fungal antagonism of Pseudomonas donghuensis strain SVBP6

Muzio

Agaras

Masi

et al. 2020

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…The annotated genome of strain P482 (JHTS00000000.1; Krzyzanowska et al, 2014 ) was searched for the presence of genes involved in the production of 26 metabolites (16 antimicrobials, six siderophores, two biosurfactants, and two compounds with unknown function) reported in literature to be produced by Pseudomonas spp. (Table S3 ).…”

Section: Methodsmentioning

confidence: 99%

“…entomophila L48 T (CT573326), and P. putida KT2440 (AE015451). For the purpose of the analysis, the genomes of HYS T , L48 T and KT2440 were re-annotated with the IGS Annotation pipeline, to match the annotation previously obtained for P482 (Krzyzanowska et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

When Genome-Based Approach Meets the “Old but Good”: Revealing Genes Involved in the Antibacterial Activity of Pseudomonas sp. P482 against Soft Rot Pathogens

et al. 2016

Self Cite

View full text Add to dashboard Cite

Dickeya solani and Pectobacterium carotovorum subsp. brasiliense are recently established species of bacterial plant pathogens causing black leg and soft rot of many vegetables and ornamental plants. Pseudomonas sp. strain P482 inhibits the growth of these pathogens, a desired trait considering the limited measures to combat these diseases. In this study, we determined the genetic background of the antibacterial activity of P482, and established the phylogenetic position of this strain. Pseudomonas sp. P482 was classified as Pseudomonas donghuensis. Genome mining revealed that the P482 genome does not contain genes determining the synthesis of known antimicrobials. However, the ClusterFinder algorithm, designed to detect atypical or novel classes of secondary metabolite gene clusters, predicted 18 such clusters in the genome. Screening of a Tn5 mutant library yielded an antimicrobial negative transposon mutant. The transposon insertion was located in a gene encoding an HpcH/HpaI aldolase/citrate lyase family protein. This gene is located in a hypothetical cluster predicted by the ClusterFinder, together with the downstream homologs of four nfs genes, that confer production of a non-fluorescent siderophore by P. donghuensis HYST. Site-directed inactivation of the HpcH/HpaI aldolase gene, the adjacent short chain dehydrogenase gene, as well as a homolog of an essential nfs cluster gene, all abolished the antimicrobial activity of the P482, suggesting their involvement in a common biosynthesis pathway. However, none of the mutants showed a decreased siderophore yield, neither was the antimicrobial activity of the wild type P482 compromised by high iron bioavailability. A genomic region comprising the nfs cluster and three upstream genes is involved in the antibacterial activity of P. donghuensis P482 against D. solani and P. carotovorum subsp. brasiliense. The genes studied are unique to the two known P. donghuensis strains. This study illustrates that mining of microbial genomes is a powerful approach for predictingthe presence of novel secondary-metabolite encoding genes especially when coupled with transposon mutagenesis.

show abstract

Uncovering the novel and putative immunogenic targets: Utilizing a reverse vaccinology approach against Fusobacterium nucleatum

Fereshteh,

Noori Goodarzi,

Bolourchi

et al. 2023

Informatics in Medicine Unlocked

View full text Add to dashboard Cite

Genome Sequence of Pseudomonas sp. Strain P482, a Tomato Rhizosphere Isolate with Broad-Spectrum Antimicrobial Activity

Cited by 15 publications

References 6 publications

7‐hydroxytropolone is the main metabolite responsible for the fungal antagonism of Pseudomonas donghuensis strain SVBP6

7‐hydroxytropolone is the main metabolite responsible for the fungal antagonism of Pseudomonas donghuensis strain SVBP6

When Genome-Based Approach Meets the “Old but Good”: Revealing Genes Involved in the Antibacterial Activity of Pseudomonas sp. P482 against Soft Rot Pathogens

Uncovering the novel and putative immunogenic targets: Utilizing a reverse vaccinology approach against Fusobacterium nucleatum

Contact Info

Product

Resources

About