Genome Sequence of
            <i>Erysipelothrix</i>
            sp. Strain Poltava, Isolated from Acute Septic Erysipelas of Swine in Ukraine

Tarasov, O.A.; Kovalenko, Ganna; Muzykina, Larysa; Bezymennyi, Maksym; Bortz, Eric; Drown, Devin M.

doi:10.1128/mra.00438-22

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…Two recently deposited (2022) non-target amplicons were identified: E. amsterdamensis and E. poltava, both exhibiting a high degree of similarity to E. rhusiopathiae (91.5% and 98.8%, respectively). Erysipelothrix amsterdamensis was characterized by Zhong et al (2022, unpublished) and isolated from deceased albatross chicks on Amsterdam Island and E. poltava from septic swine in the Ukraine (Tarasov et al, 2022). With two or fewer mismatches in each of the primer probe regions, both non-target species are indistinguishable from E. rhusiopathiae in the presented assay.…”

Section: E Rhusiopathiaementioning

confidence: 82%

“…Erysipelothrix amsterdamensis was characterized by Zhong et al. ( 2022 , unpublished) and isolated from deceased albatross chicks on Amsterdam Island and E. poltava from septic swine in the Ukraine (Tarasov et al., 2022 ). With two or fewer mismatches in each of the primer probe regions, both non‐target species are indistinguishable from E. rhusiopathiae in the presented assay.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A new multiplexed magnetic capture—Droplet digital PCR tool for monitoring wildlife population health and pathogen surveillance

Tschritter,

V. C. de Groot,

Branigan

et al. 2023

Ecology and Evolution

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Anthropogenic stressors are exacerbating the emergence and spread of pathogens worldwide. In regions like the Arctic, where ecosystems are particularly susceptible, marked changes are predicted in regional diversity, intensity, and patterns of infectious diseases. To understand such rapidly changing host‐pathogen dynamics and mitigate the impacts of novel pathogens, we need sensitive disease surveillance tools. We developed and validated a novel multiplexed, magnetic capture, and ddPCR tool for the surveillance of multiple pathogens in polar bears, a sentinel species that is considered susceptible to climate change and other stressors with a pan‐Arctic distribution. Through sequence‐specific magnetic capture, we concentrated five target template sequences from three zoonotic bacteria (Erysipelothrix rhusiopathiae, Francisella tularensis, and Mycobacterium tuberculosis complex) and two parasitic (Toxoplasma gondii and Trichinella spp.) pathogens from large quantities (<100 g) of host tissue. We then designed and validated two multiplexed probe‐based ddPCR assays for the amplification and detection of the low‐concentration target DNA. Validations used 48 polar bear tissues (muscle and liver). We detected 14, 1, 3, 4, and 22 tissue positives for E. rhusiopathiae, F. tularensis, M. tuberculosis complex, T. gondii, and Trichinella spp., respectively. These multiplexed assays offer a rapid, specific tool for quantifying and monitoring the changing geographical and host distributions of pathogens relevant to human and animal health.

show abstract

Section: E Rhusiopathiaementioning

confidence: 82%

Section: Resultsmentioning

confidence: 99%

A new multiplexed magnetic capture—Droplet digital PCR tool for monitoring wildlife population health and pathogen surveillance

Tschritter,

V. C. de Groot,

Branigan

et al. 2023

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…Two recently deposited (2022) non-target amplicons were identified; E. amsterdamensis and E. poltava , both exhibiting a high degree of similarity to E. rhusiopathiae (91.5% and 98.8% respectively). Erysipelothrix amsterdamensis was characterized in Zhong et al, (2022 unpublished) isolated from deceased albatross chicks on Amsterdam Island and E. poltava from septic swine in the Ukraine (Tarasov et al, 2022). With two or less mismatches in each of the primer probe regions, both non-target species are indistinguishable from E. rhusiopathiae in the presented assay.…”

Section: Resultsmentioning

confidence: 99%

A new multiplexed magnetic-capture - droplet digital PCR (ddPCR) tool for monitoring wildlife population health and pathogen surveillance

Tschritter,

de Groot,

Branigan

et al. 2023

Preprint

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Anthropogenic stressors are exacerbating the emergence and spread of pathogens worldwide. In regions like the Arctic where ecosystems are particularly susceptible, marked changes are predicted in regional diversity, intensity, and patterns of infectious diseases. To understand such rapidly changing host-pathogen dynamics and mitigate the impacts of novel pathogens, we need sensitive disease surveillance tools. We developed and validated a novel multiplexed, magnetic-capture and ddPCR tool for the surveillance of multiple pathogens in polar bears, a sentinel species that is considered susceptible to climate change and other stressors with a pan-Arctic distribution. Through sequence-specific magnetic capture, we concentrated five target template sequences from three zoonotic bacteria (Erysipelothrix rhusiopathiae, Francisella tularensis, and Mycobacterium tuberculosis complex) and two parasitic (Toxoplasma gondii and Trichinella spp.) pathogens from large quantities (< 100 g) of host tissue. We then designed and validated two multiplexed probe-based ddPCR assays for the amplification and detection of the low concentration target DNA. Validations used 48 polar bear tissues (muscle and liver). We detected 14, 1, 3, 4, and 22 tissue positives for E. rhusiopathiae, F. tularensis, Mycobacterium tuberculosis complex, T. gondii, and Trichinella spp., respectively. These multiplexed assays offer a rapid, specific tool for quantifying and monitoring the changing geographical and host distributions of pathogens relevant to human and animal health.

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Application of PCR and PCR-Based Techniques in Veterinary Medicine

Gerilovych,

Chechet,

Kovalenko

et al. 2023

OneHealthJournal

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New tests for the detection and typing of animal pathogens have been developed for veterinary medicine. Careful systematization is required to determine the place of molecular-based tools’ applications in the existing system of epizootological and epidemiological surveillance. Today, molecular genetic tests, including PCR, are used in veterinary medicine and agriculture for the following purposes:- surveillance and diagnosis of infectious and certain invasive diseases, - typing of animal pathogens, the study of their eco-geographic features, the drift of genetic variability and evolution, - research of molecular mechanisms of the immune response and the host-pathogen interactions, - quality and safety control of agricultural products, including food and feeds, - control of the quality and safety of genetic resources of animals, - control of the circulation of pathogens in the environment, - analysis of the origin and certification of breeds of productive and non-productive animals, etc. The application of molecular genetic methods of monitoring and early diagnosis is regulated by the Manual and Code of the World Organization for Animal Health (WOAH), the Program for the Global Control of Infectious Diseases of the World Health Organization, the guidelines on the monitoring of infectious diseases of animals and the control of the safety of agricultural products of the FAO. A large number of tests based on molecular diagnostic methods are recommended for use in infectious disease control programs, both emerging and economically significant, in the USA, Canada, and the countries of the European Union. This paper summarises the current PCR-based development scope and ways of its implementation in practical veterinary medicine.

show abstract

Genome Sequence of Erysipelothrix sp. Strain Poltava, Isolated from Acute Septic Erysipelas of Swine in Ukraine

Cited by 3 publications

References 18 publications

A new multiplexed magnetic capture—Droplet digital PCR tool for monitoring wildlife population health and pathogen surveillance

A new multiplexed magnetic capture—Droplet digital PCR tool for monitoring wildlife population health and pathogen surveillance

A new multiplexed magnetic-capture - droplet digital PCR (ddPCR) tool for monitoring wildlife population health and pathogen surveillance

Application of PCR and PCR-Based Techniques in Veterinary Medicine

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