Genome Sequence of Bacillus subtilis subsp. spizizenii gtP20b, Isolated from the Indian Ocean

Abstract

Bacillus subtilis is an aerobic spore-forming Gram-positive bacterium that is a model organism and of great industrial significance as the source of diverse novel functional molecules. Here we present, to our knowledge, the first genome sequence of Bacillus subtilis strain gtP20b isolated from the marine environment. A subset of candidate genes and gene clusters were identified, which are potentially involved in production of diverse functional molecules, like novel ribosomal and nonribosomal antimicrobial peptides. The genome sequence described in this paper is due to its high strain specificity of great importance for basic as well as applied researches on marine organisms.

Isolation, diversity, and biotechnological potential of rhizo- and endophytic bacteria associated with mangrove plants from Saudi Arabia

“…Most of the isolates that inhibited the growth of fungal pathogens in our study were related to Bacillus. Marine bacteria from this genus are already known for their antimicrobial activity against different pathogens and synthesize different classes of antibiotics (Fan et al, 2011). In addition, marine Bacillus produces different bioactive metabolites with novel structures and modes of action, which are pivotal for treatment of various human infections (Mondol et al, 2013).…”

Diversity of Secondary Metabolites from Marine Bacillus Species: Chemistry and Biological Activity

“…Many Bacillus species can also be found in both terrestrial and marine environments. This bacterial genus could be considered as one of the major sources of potential microbial biopesticides because of its broad genetic biodiversity [22,36], its large body of literary evidence, as well as generally regarded as safe by the US Food and Drug Administration (USFDA) and capable of withstanding unfavorable conditions through the formation of resistant spores. The main specific mechanisms involved in biocontrol of plant diseases by this bacterial genus include: competition for ecological niche/substrate in the rhizosphere, production of inhibitory chemicals, and induction of so-called systemic resistance in host plants.…”

“…Similarly, genome analysis of a marine B. subtilis subsp. spizisenii strain gtP20b, isolated from the Indian Ocean, indicated the presence of huge number of genes for biosynthesis of secondary metabolites [22]. …”

Whole-Genome Sequences of Bacillus subtilis and Close Relatives

“…These genomes exhibit a high degree of proteome conservation and syntenic genome architectures (2,12,13). The synteny appears to be well conserved even in more distantly related bacilli (5,7,9). However, each strain harbors numerous strain-specific regions that are found interspersed throughout the conserved genomic backbone.…”

“…CP002905), the type strain for Bacillus subtilis subspecies spizizenii (10,14), and DV1-B-1 (20 contigs) (GenBank accession no. AFSG00000000) (15) are closely related to the recently sequenced laboratory strain W23 and marine strain gtP20b (7,18). TU-B-10 T and DV1-B-1 were isolated from soils collected near Nefta, Tunisia, and Death Valley National Monument, CA (10,14,15), respectively.…”