Genome Sequence of Bacillus Phage Saddex

Abstract

The complete genome of Bacillus phage Saddex was determined and annotated in this study. Saddex has distinct sections with similarities to other Bacillus phages, such as Kida, even though these phages were isolated more than 800 km apart by separate laboratories.

Genome Annotations of Two Bacillus Phages, Tomato and BaseballField

“…A unique truncation of the tape measure gene was found in Tomato but was not found in other closely related Bacillus phages, indicating that this gene might be an area of further interest. Five tRNAs were identified, all of which had 99 to 100% similarity to tRNAs found in other Bacillus phages ( 5 – 7 ).…”

“…Tomato was autoannotated by Geneious v2020.1 using Bacillus phage Saddex (GenBank accession number MH538193 ) ( 5 ), followed by a manual cross-check with five other Bacillus phage genomes available in GenBank ( 6 , 7 ). The genome of Tomato is 146,370 bp long with a G+C content of 38.9%.…”

“…The genome of Tomato is 146,370 bp long with a G+C content of 38.9%. Of the 200 predicted proteins, 58 had an identified function, almost all of which had analogs in other Bacillus phages ( 5 – 7 ). A unique truncation of the tape measure gene was found in Tomato but was not found in other closely related Bacillus phages, indicating that this gene might be an area of further interest.…”