Genome Sequence of Edwardsiella ictaluri 93-146, a Strain Associated with a Natural Channel Catfish Outbreak of Enteric Septicemia of Catfish

Abstract

Edwardsiella ictaluri is the cause of extensive mortalities and economic losses to the channel catfish industry of the southeast United States. Here we report the complete genome of Edwardsiella ictaluri 93-146. Whole-genome sequence analysis of E. ictaluri provides a tool for understanding the genomic regions specific to the species and the Edwardsiella genus.

Fur-Regulated Iron Uptake System of Edwardsiella ictaluri and Its Influence on Pathogenesis and Immunogenicity in the Catfish Host

“…5). Our results correlate with previous observations (67) and with the absence of know siderophore biosynthesis genes in the E. ictaluri chromosome (72). This observation reinforces the hypothesis of genome degradation during the process of host specialization of E. ictaluri to catfish, where siderophore genes might be lost.…”

“…5B). Our results correlate with previous observations (67) and with the fact that siderophore biosynthesis genes are not present in the E. ictaluri chromosome (72).…”

Use of bioluminescence mutant screening for identification of Edwardsiella ictaluri genes involved in channel catfish (Ictalurus punctatus) skin colonization

“…ictaluri strain 93-146 is a clinical isolate from diseased catfish in a commercial channel catfish production pond in Louisiana in 1993. It has been routinely used to investigate mechanisms of ESC in our laboratory, and its genome sequence was recently reported (Williams et al, 2012). This strain was transformed with pAKgfplux1, which allowed constitutive expression of the bacterial luciferase operon (Karsi and Lawrence, 2007;Karsi et al, 2006).…”

“…Sequencing of E. ictaluri has revealed the presence of genes encoding fimbriae (Williams et al, 2012). Furthermore, signaturetagged mutagenesis identified two outer membrane proteins, 193UV and 233PR, that that were linked to virulence (Thune et al, 2007).…”

Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri

“…The mutants with deletion of eseH, eseI and escD, were then built up respectively, and their roles in invasion and replication were examined in host fish cells, as well as in yellow catfish. On the other hand, as T3SS in E. ictaluri is similar to that in E. tarda in gene organization (Thune et al, 2007;Rogge and Thune, 2011;Williams et al, 2012), the T3SS in E. tarda was used to analyze the secretion and translocation of EseH, EseI and EscD in fish cells. The cellular localization of these proteins was further examined in host fish cells.…”