Genome Sequence of Carbon Dioxide-Sequestering
            <i>Serratia</i>
            sp. Strain ISTD04 Isolated from Marble Mining Rocks

Kumar, Manish; Gazara, Rajesh Kumar; Verma, Sandhya; Kumar, Madan; Verma, Praveen Kumar; Thakur, Indu Shekhar

doi:10.1128/genomea.01141-16

Cited by 17 publications

(9 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The genome size of Serratia sp. ISTD04 is 5.07 Mb with 81X coverage having GC content of 59.98%, 4,563 predicted protein-coding genes (Prokaryotic Genome Annotation Pipeline (PGAP) and Pfam annotation) and the other general genome features have also been reported earlier 17 . A Circos plot showing space efficient and clear representation of genes on the genome and the important genes and their position related to CO 2 sequestration and EPS production have been shown on the circular plot (Fig.…”

Section: Resultsmentioning

confidence: 76%

See 1 more Smart Citation

Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production

Kumar

Pandey

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp. ISTD04 was performed along with exopolysaccharide (EPS) production. Genomic analysis identified key and accessory enzymes responsible for CO2 sequestration. EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production. The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L−1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8. After optimizing process parameters, the EPS production increased more than 3 folds. The morphology of strain and elemental composition of EPS was characterized by SEM-EDX. The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR. Glucose, galactose, mannose and glucosamine are the monomers detected in the EPS. EPS was further applied for bioflocculation (kaolin test) and dye removal. The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%). The results highlight CO2 sequestration and EPS production potential of Serratia sp. ISTD04 that can be harnessed in future.

show abstract

Section: Resultsmentioning

confidence: 76%

“…The genome of Serratia sp. ISTD04 was sequenced earlier and the sequence is openly available with NCBI accession number MBDW00000000.1 17 . The detail proteomics (2D-GEL) and nano-LC-MS/MS of Serratia sp.…”

Section: Introductionmentioning

confidence: 99%

Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production

Kumar

Pandey

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…As presented in Fig. 1, we found the marine microbial communities exhibited the signi cantly impressive performance on CO 2 reduction and hydrocarbon synthesis, the total yield, the metabolite diversity, the production rate, and the consumption rate of CO 2 were obviously higher than the literatures described with soil microbial communities, pure microorganisms or enzymes [18,[35][36][37][38][39][43][44][45][46][47][48][49][50]. In ocean, the role of phytoplankton on the carbon ux (CO 2 reduction) was well acknowledged.…”

Section: Discussionmentioning

confidence: 64%

“…This insight provided the possibility of oxygenic bioreactions of hydrocarbon synthesis by aldehyde decarbonylase (AD) under anaerobic condition. Speices from Serratia genus were well characterized by chemolithotrophic xation of CO 2 which was supported by the presence of RuBisCo, carbonic anhydrase, and carboxylases [36].…”

Section: Metabolic Pathways and Key Microorganisms Involved In Carbonmentioning

confidence: 89%

“…2), Acetobacterium woodii, Moorella thermoacetica, Hydrogenotrophic methanogens, Clostridium aceticum, and autotrophic sulfatereducing prokaryotes inherently carried out WL pathway [18][19][20]. As intermediate of WL pathway, CO was detected during culturing, which is reduced from CO 2 by carbon monoxide dehydrogenase (CODH) [36,37]. Metagenomic analysis of genes encoding the enzymes CODH showed that the abundance of the taxa, including Clostridium (bin 8), Carboxydothermus (bin 14), Rhodospirillum (bin 15), Pseudomonas (bin 16) and Methanosarcina (bin 17), were more than 5%, and increased during culturing.…”

Section: Metabolic Pathways and Key Microorganisms Involved In Carbonmentioning

confidence: 99%

See 1 more Smart Citation

Photo-driven CO2 Reduction to Hydrocarbons: Carbon Flux and Energy Transforming by Marine Microbial Community

Xia¹,

Chen²,

Li³

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background: Microbial activities play a crucial role in the carbon and energy cycle of the ocean. Microbial communities in the MCP has been proposed the function of transforming carbon, yet the potential for direct CO 2 reduction to the organic carbon is rarely considered in the carbon cycle and energy transforming. Results: Here we showed a naturally inherent photo-driven bioprocess of CO 2 reduction producing C 1 -C 6 alkanes/alkenes by the marine microbial community with CO 2 consumption rate of 100.87 uM day -1 . Upon the metabolite profiles and metagenomic sequencing analysis, we revealed the mechanism of CO 2 conversion to hydrocarbons that CO 2 fixation was dominantly completed with the Calvin-Benson-Bassham cycle, tricarboxylic acid cycle, and Wood-Ljungdahl cycle, while nitrogenases, aldehyde decarbonylase and carboxylic acid reductase played the crucial roles on the hydrocarbon formations. As these insights, the pathway of CO 2 to hydrocarbons was proposed. Isolated microorganisms from the enriched community, including Pseudomonas sp. , Serratia sp. , Candidatus sp. , Clostridium sp. , Enterococcus sp ., Salmonella sp. , Rhodospirillum sp. , Thalassospira sp. , Thioclava sp. , Stenotrophomonas sp. and Desulfovibrio sp., were tentatively integrated for validating the hypothesized process. Results exhibited that the improved performance of CO 2 reduction to hydrocarbons was achieved by this group. Conclusions: This study demonstrated a natural microbial CO 2 reduction process contributing to the carbon and energy cycle in the ocean, and could provide a microbial “CO 2 -hydrogenation process” or “fischer-tropsch process” for industrial application.

show abstract

Restoring Ecosystem Services of Degraded Forests in a Changing Climate

Chaudhry

Sidhu

Paliwal

2021

Handbook of Ecological and Ecosystem Engineering

View full text Add to dashboard Cite

Genome Sequence of Carbon Dioxide-Sequestering Serratia sp. Strain ISTD04 Isolated from Marble Mining Rocks

Cited by 17 publications

References 14 publications

Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production

Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production

Photo-driven CO2 Reduction to Hydrocarbons: Carbon Flux and Energy Transforming by Marine Microbial Community

Restoring Ecosystem Services of Degraded Forests in a Changing Climate

Contact Info

Product

Resources

About