Genome Sequence of Borrelia parkeri, an Agent of Enzootic Relapsing Fever in Western North America

Barbour, Alan G.; Miller, Shelley Campeau

doi:10.1128/genomea.00018-14

Cited by 13 publications

(10 citation statements)

References 10 publications

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“…The procedures for the sequencing of genomes of Bmsl strain LB-2001, which was isolated from the blood of mice, and B. parkeri strain HR1 have been described (Barbour and Campeau Miller, 2014; Hue et al, 2013). DNA from a fresh culture harvest of B. anserina was extracted with Qiagen’s DNeasy Blood/Tissue Kit (Valencia, CA) and then treated with RNase I.…”

Section: Methodsmentioning

confidence: 99%

“…To provide additional material for phylogenetic inference, my laboratory carried out sequencing of LB-2001, a Bmsl strain and original isolate in North America (Hue et al, 2013), as well as the chromosome and much of the plasmid content of B. parkeri (Barbour and Campeau Miller, 2014). For the present study, these were added to existing chromosome and plasmid sequences of other Borrelia spp.…”

Section: Introductionmentioning

confidence: 99%

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Phylogeny of a relapsing fever Borrelia species transmitted by the hard tick Ixodes scapularis

Barbour¹

2014

Infection, Genetics and Evolution

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The discovery of Borrelia species that were related to the agents of relapsing fever but were transmitted by hard ticks rather than soft ticks challenged previous taxonomies based largely on microbe-host specificities and geographic considerations. One of these newly-identified organisms is the Borrelia miyamotoi sensu lato strain LB-2001 from North America and transmitted by Ixodes scapularis. This or related strains have been identified as the cause of human disease, but comparatively little is known about their biology or genetics. Using recently acquired chromosome sequence of LB-2001 together with database sequences and additional sequences determined here, I carried out comparisons of the several species of Borrelia, including those in the two major clades: the relapsing fever group of species and the Lyme disease group of species. Phylogenetic inference at the species level was based on four data sets: whole chromosomes of ~1 Mb each, and concatenated sequences of 19 ribosomal protein genes, 3 conserved nucleic acid enzymes (rpoC, recC, and dnaE), and 4 contiguous genes for nucleotide salvage on a large plasmid. Analyses using neighbor-joining, maximum likelihood, and Bayesian methods were largely concordant for each of the trees. They showed that LB-2001 and related hard tick-associated organisms, like B. lonestari, are deeply positioned within the RF group of species and that these organisms did not, as some earlier estimations had suggested, constitute a paraphyletic group. The analyses also provided further evidence that major changes in host ranges and life cycles, such as hard to soft ticks or vice versa, may not correlate well with overall sequence differences. The genetic differences between LB-2001 and B. miyamotoi sensu stricto justify provisional use of the “sensu lato” designation for LB-2001.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phylogeny of a relapsing fever Borrelia species transmitted by the hard tick Ixodes scapularis

Barbour¹

2014

Infection, Genetics and Evolution

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“…BLAST analysis indicated that apart from B . parkeri , a closely related North America species [ 19 , 21 ], homologues with > 42% amino acid identity were absent for most proteins encoded toward the 3’ end of lp150 ( Table 1 ). Moreover, putative functional homologues were not identified for the proteins.…”

Section: Resultsmentioning

confidence: 99%

“…Relapsing fever spirochetes are comprised of a linear chromosome, a 150 to 174 kb megaplasmid and 6–16 linear and circular plasmids that are ~10–55 kb [ 17 – 20 ]. The plasmids contain extensive regions of repetitive DNA, which has resulted in incomplete assemblies [ 21 , 22 ]. B .…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Profiling the 150 kb Linear Megaplasmid of Borrelia turicatae Suggests a Role in Vector Colonization and Initiating Mammalian Infection

et al. 2016

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Adaptation is key for survival as vector-borne pathogens transmit between the arthropod and vertebrate, and temperature change is an environmental signal inducing alterations in gene expression of tick-borne spirochetes. While plasmids are often associated with adaptation, complex genomes of relapsing fever spirochetes have hindered progress in understanding the mechanisms of vector colonization and transmission. We utilized recent advances in genome sequencing to generate the most complete version of the Borrelia turicatae 150 kb linear megaplasmid (lp150). Additionally, a transcriptional analysis of open reading frames (ORFs) in lp150 was conducted and identified regions that were up-regulated during in vitro cultivation at tick-like growth temperatures (22°C), relative to bacteria grown at 35°C and infected murine blood. Evaluation of the 3’ end of lp150 identified a cluster of ORFs that code for putative surface lipoproteins. With a microbe’s surface proteome serving important roles in pathogenesis, we confirmed the ORFs expression in vitro and in the tick compared to spirochetes infecting murine blood. Transcriptional evaluation of lp150 indicates the plasmid likely has essential roles in vector colonization and/or initiating mammalian infection. These results also provide a much needed transcriptional framework to delineate the molecular mechanisms utilized by relapsing fever spirochetes during their enzootic cycle.

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“…The single-molecule real-time (SMRT) long-read approach ( 6 ) on a PacBio RS I instrument (Pacific Biosciences, Menlo Park, CA) was combined with the short paired-end read approach on an Illumina (Hayward, CA) HiSeq 2500 instrument, as described in reference 7 , for sequencing the chromosome, linear megaplasmid, linear plasmids, and circular plasmids of the “Browne Mountain” isolate of type strain HS1 (BioProject PRJNA311246 and BioSample SAMN04481062) of B. hermsii . DNA extracted with the Qiagen (Valencia, CA) Midi kit from cells grown in BSK II medium ( 3 ) was sheared to 20 to 50 kb for library preparation.…”

Section: Genome Announcementmentioning

confidence: 99%

Chromosome and Plasmids of the Tick-Borne Relapsing Fever Agent Borrelia hermsii

Barbour

2016

Genome Announc

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Genome Sequence of Borrelia parkeri, an Agent of Enzootic Relapsing Fever in Western North America

Cited by 13 publications

References 10 publications

Phylogeny of a relapsing fever Borrelia species transmitted by the hard tick Ixodes scapularis

Phylogeny of a relapsing fever Borrelia species transmitted by the hard tick Ixodes scapularis

Transcriptional Profiling the 150 kb Linear Megaplasmid of Borrelia turicatae Suggests a Role in Vector Colonization and Initiating Mammalian Infection

Chromosome and Plasmids of the Tick-Borne Relapsing Fever Agent Borrelia hermsii

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