Genome Sequence of Airborne
 Acinetobacter
 sp. Strain 5-2Ac02 in the Hospital Environment, Close to the Species of Acinetobacter towneri

Barbosa, Beathriz Godoy Vilela; Fernández-García, Laura; Gato, Eva; López, María; Blasco, Lucía; Leão, Robson Souza; Albano, Rodolpho Mattos; Fernández, B.; Cuenca, F Fernández; Pascual, Álvaro; Bou, Germán; Marques, Elizabeth Andrade; Tomás, María

doi:10.1128/genomea.01343-16

Cited by 3 publications

(7 citation statements)

References 9 publications

(8 reference statements)

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“…The data presented in Table 1 show that Acinetobacter sp. 5-2Ac02 is susceptible to all antibiotic tested but resistant to copper as well as to arsenic, as previously reported (Barbosa et al, 2016). This information was confirmed by growth curves in the presence of these heavy metals (Supplementary Figure S1).…”

Section: Resultssupporting

confidence: 84%

“…The high arsenic MIC for Acinetobacter sp. strain 5-2Ac02 may be attributed to the arsenic operon, arsC1–arsR–arsC2–ACR–arsH, which has only been described in the Pseudomonas stutzeri TS44 (Barbosa et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…We further analyzed the global gene expression adjustments in this strain in response to environmental stressors such as mitomycin C and found induction of genes coding for components of the SOS response, genes involved in numerous TA systems (RelBE, HigBA, parDE, and other two new TA systems) (Barbosa et al, 2016), and resistance to heavy metals and antioxidant enzymes. The TA systems have been shown to be involved both in tolerance and persistence, which presuppose the ability of the bacteria to grow slowly or enter into a dormant state, respectively, to cope with the presence of a stressor (Fernandez-Garcia et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…We have recently reported the genome sequence of Acinetobacter sp. strain 5-2Ac02 (closely related to Acinetobacter towneri ), which has been recovered from the air in an ICU of a hospital in Rio de Janeiro, Brazil (Barbosa et al, 2016). This strain was shown to harbor a much reduced genome and higher content of insertion sequences than other Acinetobacter sp.…”

Section: Introductionmentioning

confidence: 99%

“…This strain was shown to harbor a much reduced genome and higher content of insertion sequences than other Acinetobacter sp. Moreover, four different toxin–antitoxin (TA) systems as well as heavy metal resistance operons were found encoded in its genome (Barbosa et al, 2016). Interestingly, some bacteria have been shown to produce and release a large diversity of small molecules, including organic and inorganic volatile compounds such as acetoin and 2,3-butanediol (BD), referred as bacterial volatile compounds (BVCs), which can mediate airborne bacterial interactions (Audrain et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Quorum and Light Signals Modulate Acetoin/Butanediol Catabolism in Acinetobacter spp.

et al. 2019

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Acinetobacter spp. are found in all environments on Earth due to their extraordinary capacity to survive in the presence of physical and chemical stressors. In this study, we analyzed global gene expression in airborne Acinetobacter sp. strain 5-2Ac02 isolated from hospital environment in response to quorum network modulators and found that they induced the expression of genes of the acetoin/butanediol catabolism, volatile compounds shown to mediate interkingdom interactions. Interestingly, the acoN gene, annotated as a putative transcriptional regulator, was truncated in the downstream regulatory region of the induced acetoin/butanediol cluster in Acinetobacter sp. strain 5-2Ac02, and its functioning as a negative regulator of this cluster integrating quorum signals was confirmed in Acinetobacter baumannii ATCC 17978. Moreover, we show that the acetoin catabolism is also induced by light and provide insights into the light transduction mechanism by showing that the photoreceptor BlsA interacts with and antagonizes the functioning of AcoN in A. baumannii , integrating also a temperature signal. The data support a model in which BlsA interacts with and likely sequesters AcoN at this condition, relieving acetoin catabolic genes from repression, and leading to better growth under blue light. This photoregulation depends on temperature, occurring at 23°C but not at 30°C. BlsA is thus a dual regulator, modulating different transcriptional regulators in the dark but also under blue light, representing thus a novel concept. The overall data show that quorum modulators as well as light regulate the acetoin catabolic cluster, providing a better understanding of environmental as well as clinical bacteria.

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Section: Resultssupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quorum and Light Signals Modulate Acetoin/Butanediol Catabolism in Acinetobacter spp.

et al. 2019

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An NDM-1-Producing Acinetobacter towneri Isolate from Hospital Sewage in China

Wang

et al. 2020

IDR

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Background: The New Delhi metallo-β-lactamase-1 (NDM-1)-positive plasmid and its variants pose daunting threats to public health. Hospital sewage was considered as an important reservoir of antibiotic genes. Numerous and diverse taxa of multidrug-resistant (MDR) bacteria carrying NDM-1-positive plasmids have been identified during routine surveillance of hospital sewage. We herein report a carbapenem-resistant Acinetobacter towneri strain AeBJ009 with an NDM-1-positive plasmid isolated from hospital sewage. Materials and Methods: Bacteria were isolated from cultures of hospital sewage and identified by using the Vitek 2 compact system and 16S rRNA sequencing. The bla NDM-1 gene was amplified and confirmed by sequencing. Antimicrobial susceptibility testing was performed using AST-GN14 on the Vitek2 compact system. In addition, the bla NDM-1 gene was located by Southern blotting. Conjugation experiment and whole-genome sequencing were performed for further analysis. Results: Strain AeBJ009 was isolated from hospital sewage and identified as A. towneri. Antimicrobial susceptibility testing revealed an MDR phenotype. Pulsed-field gel electrophoresis and Southern blotting showed that strain AeBJ009 carries three plasmids and that bla NDM-1 is located on the 47kb plasmid pNDM-AeBJ009. However, the conjugation experiment to transfer pNDM-AeBJ009 to Escherichia coli strain J53 was unsuccessful. Whole-genome sequencing found that pNDM-AeBJ009 contains a Tn125 element carrying bla NDM-1 . The ble gene downstream of bla NDM-1 displayed a single-nucleotide polymorphism compared to its homologue on plasmid pM131_NDM1. BLAST analysis using the Comprehensive Antibiotic Resistance Database identified no gene polymorphisms with 100% identity to our ble variant. Conclusion: The A. towneri strain AeBJ009 exhibiting an extended spectrum of antibiotic resistance was isolated from hospital sewage and may potentially exacerbate the risk of MDR bacterial infections. The prevention of nosocomial infections due to drug-resistant bacteria will require enhanced monitoring and control of MDR pathogens in environmental reservoirs.

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Maternal Psychosocial Stress Is Associated with Reduced Diversity in the Early Infant Gut Microbiome

et al. 2023

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The developing infant gut microbiome is highly sensitive to environmental exposures, enabling its evolution into an organ that supports the immune system, confers protection from infection, and facilitates optimal gut and central nervous system function. In this study, we focus on the impact of maternal psychosocial stress on the infant gut microbiome. Forty-seven mother–infant dyads were recruited at the HEAL Africa Hospital in Goma, Democratic Republic of Congo. Extensive medical, demographic, and psychosocial stress data were collected at birth, and infant stool samples were collected at six weeks, three months, and six months. A composite maternal psychosocial stress score was created, based on eight questionnaires to capture a diverse range of stress exposures. Full-length 16S rRNA gene sequences were generated. Infants of mothers with high composite stress scores showed lower levels of gut microbiome beta diversity at six weeks and three months, as well as higher levels of alpha diversity at six months compared to infants of low stress mothers. Longitudinal analyses showed that infants of high stress mothers had lower levels of health-promoting Lactobacillus gasseri and Bifidobacterium pseudocatenulatum at six weeks compared to infants of low stress mothers, but the differences largely disappeared by three to six months. Previous research has shown that L. gasseri can be used as a probiotic to reduce inflammation, stress, and fatigue, as well as to improve mental state, while B. pseudocatenulatum is important in modulating the gut–brain axis in early development and in preventing mood disorders. Our finding of reduced levels of these health-promoting bacteria in infants of high stress mothers suggests that the infant gut microbiome may help mediate the effect of maternal stress on infant health and development.

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Genome Sequence of Airborne Acinetobacter sp. Strain 5-2Ac02 in the Hospital Environment, Close to the Species of Acinetobacter towneri

Cited by 3 publications

References 9 publications

Quorum and Light Signals Modulate Acetoin/Butanediol Catabolism in Acinetobacter spp.

Quorum and Light Signals Modulate Acetoin/Butanediol Catabolism in Acinetobacter spp.

<p>An NDM-1-Producing <em>Acinetobacter towneri</em> Isolate from Hospital Sewage in China</p>

Maternal Psychosocial Stress Is Associated with Reduced Diversity in the Early Infant Gut Microbiome

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