Genome Sequence of a Polydnavirus: Insights into Symbiotic Virus Evolution

Espagne, Eric; Dupuy, Catherine; Huguet, Elisabeth; Cattolico, Laurence; Provost, Bertille; Martins, Nathalie; Poirié, Marylène; Périquet, Georges; Drezen, Jean‐Michel

doi:10.1126/science.1103066

Cited by 240 publications

(180 citation statements)

References 30 publications

(5 reference statements)

Supporting

Mentioning

176

Contrasting

Unclassified

Order By: Relevance

“…To date, the full sequences of eight PDV genomes have been published (Espagne et al, 2004;Webb et al, 2006;Tanaka et al, 2007;Desjardins et al, 2008) but almost no genes sharing similarities with known virus genes were identified. This led us to question whether PDVs were of viral origin or merely 'genetic secretions' having morphological similarity to viruses (Federici & Bigot, 2003;Espagne et al, 2004). However, we have recently demonstrated that the BVs derive from an ancestral nudivirus.…”

Section: Introductionmentioning

confidence: 99%

Identification of bracovirus particle proteins and analysis of their transcript levels at the stage of virion formation

Wetterwald

Roth

Kaeslin

et al. 2010

Journal of General Virology

View full text Add to dashboard Cite

Polydnaviruses (PDVs) are unique symbiotic viruses associated with parasitic wasps; they replicate only in the calyx cells of a wasp's ovaries and are transferred at oviposition along with the parasitoid egg into the lepidopteran host. The DNA packaged in the viral particles encodes factors that manipulate the host's immune defences and development to benefit the parasitoid. PDVs are found in two subfamilies of ichneumonids (ichnoviruses) and in braconids of the microgastroid complex (bracoviruses). We recently showed that the latter derive from an ancestral nudivirus, as 24 nudivirus-related genes were identified in ovaries of two distantly related braconids at the stage of virion formation. Here, we present a comprehensive analysis of the viral particle proteins of the Chelonus inanitus bracovirus (CiBV). Proteins of purified CiBV particles were analysed by mass spectrometry and amino acid sequences matched to the existing ovarian-cDNA database. In addition, transcript quantities of identified genes were measured by quantitative real-time PCR in female pupae at the onset and peak of virion formation and at corresponding stages in male pupae. This combined approach allowed the identification of 44 CiBV particle proteins: 16 were nudivirus-related, three had similarity to ovarian proteins of another braconid, 11 had similarity to cellular proteins and 14 had no similarity to known proteins. The transcripts of all of them increased in female, but not male, pupae. These data confirm the important contribution of nudivirus genes but also indicate the presence of many lineage-or species-specific proteins possibly involved in the parasitoid-host interaction.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of bracovirus particle proteins and analysis of their transcript levels at the stage of virion formation

Wetterwald

Roth

Kaeslin

et al. 2010

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…Within the Polydnaviridae, bracoviruses are associated with braconid wasps of the microgastroid complex (20). Parasitoid wasps have domesticated these viruses for transferring virulence genes (21)(22)(23), interfering with the immune response and development of their lepidopteran hosts (24,25). The longdebated genuine viral nature of bracoviruses has recently been demonstrated with the discovery in wasp genomes of genes involved in bracovirus particle production (26)(27)(28).…”

mentioning

confidence: 99%

Paleozoic origin of insect large dsDNA viruses

Thézé

Bézier

Périquet

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

139

View full text Add to dashboard Cite

To understand how extant viruses interact with their hosts, we need a historical framework of their evolutionary association. Akin to retrovirus or hepadnavirus viral fossils present in eukaryotic genomes, bracoviruses are integrated in braconid wasp genomes and are transmitted by Mendelian inheritance. However, unlike viral genomic fossils, they have retained functional machineries homologous to those of large dsDNA viruses pathogenic to arthropods. Using a phylogenomic approach, we resolved the relationships between bracoviruses and their closest free relatives: baculoviruses and nudiviruses. The phylogeny showed that bracoviruses are nested within the nudivirus clade. Bracoviruses establish a bridge between the virus and animal worlds. Their inclusion in a virus phylogeny allowed us to relate free viruses to fossils. The ages of the wasps were used to calibrate the virus phylogeny. Bayesian analyses revealed that insect dsDNA viruses first evolved at ∼310 Mya in the Paleozoic Era during the Carboniferous Period with the first insects. Furthermore the virus diversification time frame during the Mesozoic Era appears linked to the diversification of insect orders; baculoviruses that infect larvae evolved at the same period as holometabolous insects. These results imply ancient coevolution by resource tracking between several insect dsDNA virus families and their hosts, dating back to 310 Mya.Baculoviridae | Polydnaviridae | virus age estimate | time to most recent common ancestor | paleovirology

show abstract

“…Parmi les 165 gènes, près de la moitié ne présente aucune similitude avec des gènes préalablement décrits. L'autre moitié est constituée de gènes organisés en familles multigéniques codant pour des protéines qui ressemblent à des facteurs de virulence utilisés par des bactéries pathogènes ou des nématodes parasites pour réussir leur infection [5]. Cependant, de façon inattendue, ce génome est quasiment dépourvu de gènes homologues à des gènes viraux pouvant intervenir dans la réplication, ou coder pour les protéines de structure.…”

unclassified