Genome sequence of a pathogenic Corynebacterium ulcerans strain isolated from a wild boar with necrotizing lymphadenitis

Busch, Anne; Möller, Jens; Burkovski, Andreas; Hotzel, Helmut

doi:10.1186/s13104-019-4704-3

Cited by 6 publications

(10 citation statements)

References 13 publications

(11 reference statements)

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“…We have recently isolated an atypical C. ulcerans strain, W25, associated with necrotizing lymphadenitis in a wild boar and published the genome sequence (Busch et al 2019). While the size of the genome is consistent with other C. ulcerans genomes, G ?…”

Section: Introductionmentioning

confidence: 69%

“…While the size of the genome is consistent with other C. ulcerans genomes, G ? C content of the W25 was approximately 1.0% higher than other C. ulcerans strains (Busch et al 2019). It may reflect significant variations in the gene content and virulence properties of this strain than other C. ulcerans isolates.…”

Section: Introductionmentioning

confidence: 80%

“…These results are also confirmed by other bacterial identification platforms including PathoBacTyper (Tsai et al 2017), TrueBac TM ID cloud system (www.truebacid.com) and Type (Strain) Genome Server (Meier-Kolthoff and Göker 2019). W25 was isolated from a case of necrotizing lymphadenitis in a wild boar (Busch et al 2019), PO100/5 from a pig and KL1196 from a roe deer. Therefore, this species also appears to be prevalent among animals.…”

Section: Discussionmentioning

confidence: 99%

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Phylogenomic characterisation of a novel corynebacterial species pathogenic to animals

Möller¹,

Musella²,

Mel'nikov³

et al. 2020

Antonie van Leeuwenhoek

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The genus Corynebacterium includes species of biotechnological, medical and veterinary importance. An atypical C. ulcerans strain, W25, was recently isolated from a case of necrotizing lymphadenitis in a wild boar. In this study, we have analysed the genome sequence of this strain and compared the phenotypic and virulence properties with other corynebacterial pathogens. Phylogenomic analyses revealed that strain W25 belongs to a novel species along with PO100/5 and KL1196. The latter strains were isolated from a pig and a roe deer, respectively; hence, this species appears to be associated to animals. The isolate W25 is likely a nontoxigenic tox gene bearing strain and may have compromised abilities to adhere to pharyngeal and laryngeal epithelial cells due to potential loss of the gene functions in spaBC and spaDEF pilus gene clusters. A number of corynebacterial virulence genes are present including pld encoding phospholipase D. Therefore, this strain may be able to cause severe invasive infections in animals and zoonotic infections in humans.

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Section: Introductionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Phylogenomic characterisation of a novel corynebacterial species pathogenic to animals

Möller¹,

Musella²,

Mel'nikov³

et al. 2020

Antonie van Leeuwenhoek

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“…Nevertheless, given that we can perceive a slight gray colour in the GENPPI heat map, we believe that this species has some protein similarity to C. pseudotuberculosis . In this case, the literature describes the species C. pseudotuberculosis , C. diphtheriae , and C. ulcerans as being evolutionarily related (Busch et al , 2019) (McNamara, Cuevas, and Songer 1995).…”

Section: Resultsmentioning

confidence: 99%

GENPPI: standalone software for creating protein interaction networks from genomes

Ferreira

Lanes

Azevedo

et al. 2021

Preprint

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MotivationBacterial genomes are being deposited into online databases at an increasing rate. Genome annotation represents one of the first efforts to understand organisms and their diseases. Some evolutionary relationships that are capable of being annotated only from genomes are conserved gene neighbourhoods (CNs), phylogenetic profiles (PPs), and gene fusions. At present, there is no standalone software that enables networks of interactions among proteins to be created using these three evolutionary characteristics with efficient and effective results.ResultsWe developed GENPPI software for the ab initio prediction of interaction networks using predicted proteins from a genome. In our case study, we employed 50 genomes of the genus Corynebacterium. Based on the PP relationship, GENPPI differentiated genomes between the ovis and equi biovars of the species Corynebacterium pseudotuberculosis and created groups among the other species analysed. If we inspected only the CN relationship, we could not entirely separate biovars, only species. Our software GENPPI was determined to be efficient because, for example, it creates interaction networks from the central genomes of 50 species/lineages with an average size of 2200 genes in less than 40 minutes on a conventional computer. Our software is compelling because the interaction networks that it creates reflect evolutionary relationships among species and were obtained in average nucleotide identity (ANI) analyses. Additionally, this software enables the user to define how he or she intends to explore the PP and CN characteristics through various parameters, enabling the creation of customized interaction networks. For instance, users can set parameters regarding the genus, metagenome, or pangenome. In addition to the parameterization of GENPPI, it is also the user’s choice regarding which set of genomes he or she is going to study.AvailabilityThe source code in the Common Lisp language, binary files for different operating systems, and GENPPI software tutorials are available at {{github.com/santosardr/genppi}}.Contactsantosardr@ufu.brSupplementary informationSupplementary data are available at Bioinformatics online.

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“…Recently the genome sequence of the atypical C. ulcerans strain W25, isolated from a wild boar with necrotizing lymphadenitis, was published [ 21 ]. Taxonomic analyses of the genome sequence data revealed that this isolate is clustered with two different isolates previously annotated as C. ulcerans KL1196 (identical to CVUAS6455 [ 17 ]) and C. pseudotuberculosis PO100/5 [ 22 ] and clearly delineated from other published C. ulcerans strains [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Cellular and Extracellular Proteome of the Animal Pathogen Corynebacterium silvaticum, a Close Relative of Zoonotic Corynebacterium ulcerans and Corynebacterium pseudotuberculosis

et al. 2020

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Corynebacterium silvaticum is a newly described animal pathogen, closely related to the emerging human pathogen Corynebacterium ulcerans and Corynebacterium pseudotuberculosis, a major pathogen of small ruminants. In this study, proteins of a whole cell and a shaving fraction and the exoproteome of C. silvaticum strain W25 were analyzed as a first proteome study of this species. In total, 1305 proteins were identified out of 2013 proteins encoded by the W25 genome sequence and number of putative virulence factors were detected already under standard growth conditions including phospholipase D and sialidase. An up to now uncharacterized trypsin-like protease is by far the most secreted protein in this species, indicating a putative role in pathogenicity. Furthermore, the proteome analyses carried out in this study support the recently published taxonomical delineation of C. silvaticum from the closely related zoonotic Corynebacterium species.

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Genome sequence of a pathogenic Corynebacterium ulcerans strain isolated from a wild boar with necrotizing lymphadenitis

Cited by 6 publications

References 13 publications

Phylogenomic characterisation of a novel corynebacterial species pathogenic to animals

Phylogenomic characterisation of a novel corynebacterial species pathogenic to animals

GENPPI: standalone software for creating protein interaction networks from genomes

Cellular and Extracellular Proteome of the Animal Pathogen Corynebacterium silvaticum, a Close Relative of Zoonotic Corynebacterium ulcerans and Corynebacterium pseudotuberculosis

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