Genome Sequence of a Novel H10N9 Avian Influenza Virus Isolated from Chickens in a Live Poultry Market in Eastern China

Abstract

An H10N9 avian influenza virus (AIV) strain, A/Chicken/Jiangsu/RD5/2013, was isolated in China. The hemagglutinin (HA) and neuraminidase (NA) genes in this strain originated from H10N1 and H7N9 AIVs, respectively, and the other genes derived from H7N3 AIVs. Sequence analysis implies that the H10N9 AIV may be an NA gene donor for the human H7N9 influenza viruses.

Novel reassortant H10N7 avian influenza viruses isolated from chickens in Eastern China

“…Values represent mean ± SD. circulated in China since 2007 and undergone frequent reassortment with multiple other subtypes, including H6, H7, and H9 [4,[11][12][13]. Based on their genetic makeup, the ZJ-2C66, ZJ-2CP2, and ZJ-2CP8 strains are reassortant viruses from poultry H10, H2, H7, and H3 subtypes of poultry in Eastern China, and provide additional evidence for active evolution and segment reassortment in the H10 AIVs.…”

A new piece in the puzzle of the novel avian-origin influenza A (H7N9) virus

“…Lam et al also highlight H11N9 and H2N9 precursors for the NA segment that were more closely related to the H7N9 causing recent human infections than the initially mentioned KO14-like (H7N9) viruses. However, we would like to draw attention to a new development which is the identification of a different closest precursor strain for the NA segment by Su et al [ 6 ] which became available only after the first analyses were published but was not mentioned by Lam et al . Contemporary to the H7N9 outbreak, A/chicken/Jiangsu/RD5/2013(H10N9) was collected in March 2013 from a live poultry market in Jiangsu and the genome submitted to Genbank [ 7 ] in May by Su et al who also reported a quick characterization without phylogenetic analysis [ 6 ].…”

“…However, we would like to draw attention to a new development which is the identification of a different closest precursor strain for the NA segment by Su et al [ 6 ] which became available only after the first analyses were published but was not mentioned by Lam et al . Contemporary to the H7N9 outbreak, A/chicken/Jiangsu/RD5/2013(H10N9) was collected in March 2013 from a live poultry market in Jiangsu and the genome submitted to Genbank [ 7 ] in May by Su et al who also reported a quick characterization without phylogenetic analysis [ 6 ]. We have examined the detailed phylogenetic relationship of the NA of this new strain (hereafter referred to as JS5-like (H10N9)) using a maximum-likelihood tree (employing a gamma-distributed HKY85 substitution model [ 8 ] and 1000-replicate bootstrapping [ 9 ]) using MAFFT [ 10 ], Jalview [ 11 ] and PhyML [ 12 ] (Figure 1 A) and confirmed that this JS5-like N9 NA was indeed more closely related to the H7N9 outbreak sequences than the previously postulated closest KO14-like (H7N9) ancestor and the H11N9 or H2N9 strains listed by Lam et al .…”

Isolation and characterization of a novel H10N2 avian influenza virus from a domestic duck in Eastern China

“…2 and Table S1 (supplementary material). Previous reports showed that the HA genes of H10 AIVs that have been introduced to poultry in Eastern China, and these H10 viruses all underwent frequent reassortment with multiple virus subtypes, including H7 and H9 Hai-bo et al, 2012b;Su et al, 2013). According to the above-described analysis, ZJ-6D20 was a reassortant virus and received its genes from H10, H1 and H7 subtype viruses of poultry and wild birds in Eastern Asia.…”