Genome Sequence of a Human Norovirus GII.4 Hong Kong[P31] Variant in Hong Kong, China

Tse, Erin H Y; Zhang, Lin-Yao; Lau, Sin-Leung; Chan, Martin C.W.

doi:10.1128/mra.01391-19

Cited by 3 publications

(3 citation statements)

References 8 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GII⋅13P [16] and GII⋅3P [16] were detected in Italy over the years 2007-2013(Medici et al, 2014. In our present study of children with AGE in Indonesia, GII⋅13P [16] was identified mostly in 2015 (Tse et al, 2020). GII.6[P7], which was identified in outbreaks in Australia in 2012-2014 (Bruggink et al, 2016), was detected in samples from 2015 and 2018 in the present study.…”

Section: Discussionsupporting

confidence: 59%

Molecular epidemiology and genetic diversity of norovirus infection in children hospitalized with acute gastroenteritis in East Java, Indonesia in 2015–2019

Utsumi

Lusida

Dinana

et al. 2021

Infection, Genetics and Evolution

View full text Add to dashboard Cite

Noroviruses are recognized as a leading cause of outbreaks and sporadic cases of acute gastroenteritis (AGE) among individuals of all ages worldwide, especially in children <5 years old. We investigated the epidemiology of noroviruses among hospitalized children at two hospitals in East Java, Indonesia. Stool samples were collected from 966 children with AGE during September 2015-July 2019. All samples were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) for the amplification of both the RNA-dependent RNA polymerase (RdRp) and the capsid genes of noroviruses. The genotypes were determined by phylogenetic analyses. In 2015-2019, noroviruses were detected in 12.3% (119/966) of the samples. Children <2 years old showed a significantly higher prevalence than those ≥2 years old (P = 0.01). NoV infections were observed throughout the year, with the highest prevalence in December. Based on our genetic analyses of RdRp, GII.[P31] (43.7%, 31/71) was the most prevalent RdRp genotype, followed by GII.[P16] (36.6%, 26/71). GII.[P31] was a dominant genotype in 2016 and 2018, whereas GII.[P16] was a dominant genotype in 2015 and 2017. Among the capsid genotypes, the most predominant norovirus genotype from 2015 to 2018 was GII.4 Sydney_2012 (33.6%, 40/ 119). The most prevalent genotype in each year was GII.13 in 2015, GII.4 Sydney_2012 in 2016 and 2018, and GII.3 in 2017. Based on the genetic analyses of RdRp and capsid sequences, the strains were clustered into 13 RdRp/capsid genotypes; 12 of them were discordant, e.g., GII.4 Sydney[P31], GII.3[P16], and GII.13[P16]. The predominant genotype in each year was GII.13[P16] in 2015, GII.4 Sydney[P31] in 2016, GII.3[P16] in 2017, and GII.4 Sydney[P31] in 2018. Our results demonstrate high detection rates and genetic diversity of norovirus GII genotypes in pediatric AGE samples from Indonesia. These findings strengthen the importance of the continuous molecular surveillance of emerging norovirus strains.

show abstract

Section: Discussionsupporting

confidence: 59%

Molecular epidemiology and genetic diversity of norovirus infection in children hospitalized with acute gastroenteritis in East Java, Indonesia in 2015–2019

Utsumi

Lusida

Dinana

et al. 2021

Infection, Genetics and Evolution

View full text Add to dashboard Cite

show abstract

“…This finding suggested that evolution patterns may be conserved, overall, but they could also be different in a specific population in a specific area. It was noted that the new variant Hong_Kong_2019 that was first isolated from a female patient with AGEs in August 2019 shared 92.6% amino acid sequence homology with capsid of the variant Osaka_2007 (GenBank: GQ845369) [ 34 ]. Considering that variant Sydney_2012 has been predominant since 2012, the emergence of variant Hong_Kong_2019 may indicate a new round of variant replacement.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Evolution of Noroviruses between 1966 and 2019, Implications for Vaccine Design

et al. 2021

View full text Add to dashboard Cite

Noroviruses (NoVs), a group of single-stranded RNA viruses causing epidemic acute gastroenteritis in humans, are highly diverse, consisting of multiple genogroups with >30 genotypes. Their continual evolutions make NoV vaccine design and development difficult. Here, we report a study of NoV sequences obtained from a population-based diarrhea surveillance in Zhengding County of Hebei Province spanning from 2001 to 2019 and those available in the GenBank database from 1966 to 2019. NoV genotypes and/or variants that may evade immunity were screened and identified based on primary and conformational structures for vaccine design. We selected 366, 301, 139, 74 and 495 complete VP1-coding nucleotide sequences representing the predominant genotypes of GII.4, GII.2, GII.3, GII.6 and GII.17, respectively. A total of 16 distinct GII.4 variants were identified, showing a typical linear evolutionary pattern of variant replacement, while only 1–4 variants of the other genotypes were found to co-circulate over the 40–50-year period without typical variant replacement. The vaccine strain GII.4c is close to variant Sydney_2012 (0.053) in their primary structure, but they are distinct at epitopes A and E in conformations. Our data suggested GII.4 variant Sydney_2012, GII.2 variant A, a GII.3 strain, GII.6 variants B and C and GII.17 variant D are primary candidate strains for NoV vaccine development.

show abstract

“…We recently reported the full genome of a new norovirus GII.4 variant called GII.4 Hong Kong, discovered from the local molecular surveillance of norovirus gastroenteritis in hospitalized patients in Hong Kong, China ( 12 ). The strain Hu/HK/2019/GII.4 Hong Kong[P31]/CUHK-NS-2200 was collected in August 2019.…”

Section: The Studymentioning

confidence: 99%

Detection of Norovirus Variant GII.4 Hong Kong in Asia and Europe, 2017−2019

Chan¹,

Roy²,

Bonifacio³

et al. 2021

Emerg. Infect. Dis.

Self Cite

View full text Add to dashboard Cite

show abstract

Genome Sequence of a Human Norovirus GII.4 Hong Kong[P31] Variant in Hong Kong, China

Cited by 3 publications

References 8 publications

Molecular epidemiology and genetic diversity of norovirus infection in children hospitalized with acute gastroenteritis in East Java, Indonesia in 2015–2019

Molecular epidemiology and genetic diversity of norovirus infection in children hospitalized with acute gastroenteritis in East Java, Indonesia in 2015–2019

Prevalence and Evolution of Noroviruses between 1966 and 2019, Implications for Vaccine Design

Detection of Norovirus Variant GII.4 Hong Kong in Asia and Europe, 2017−2019

Contact Info

Product

Resources

About