Genome Sequence of a Heterotrophic Nitrifier and Aerobic Denitrifier, Paracoccus denitrificans Strain ISTOD1, Isolated from Wastewater

Medhi, Kristina; Mishra, Arti; Thakur, Indu Shekhar

doi:10.1128/genomea.00210-18

Cited by 12 publications

(5 citation statements)

References 10 publications

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“…This enables them to survive in aerobic or anaerobic conditions, which explains their ongoing abundance in all three reactors. Paracoccus are well known for their denitrification and phosphorus removal capabilities [39,40]. Thauera were identified at 6.3% in R1, 19% in R2, and 12% in R3 on day 11, and decreased after that.…”

Section: Distribution Of the Microbial Profilementioning

confidence: 99%

Phosphorus Removal from Aerobic Granular Sludge: Proliferation of Polyphosphate-Accumulating Organisms (PAOs) under Different Feeding Strategies

et al. 2022

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Aerobic granular sludge (AGS) is known for high phosphorus removal from wastewaters, and phosphorus can be recovered from high phosphorus-containing waste sludge granules. This study aimed at determining the feeding strategy that provides the best performance in terms of the proliferation of polyphosphate-accumulating organisms (PAOs) and phosphorus removal. Using three AGS bioreactors, this study compared phosphorus removal and the proliferation dynamics of PAOs under three different feeding strategies: anaerobic slow feeding (R1), pulse feeding + anaerobic mixing (R2), and pulse feeding (R3). Results indicate that R1 and R2 achieved significantly higher phosphorus removal (97.6 ± 3% for R1 and 98.3 ± 1% for R2) than R3 (55 ± 11%). The anaerobic slow feeding procedure (R1) achieved the highest specific phosphorus release rate (SPRR) and specific phosphorus uptake rate (SPUR) as compared to the other two feeding conditions. 16S ribosomal ribonucleic acid (rRNA) gene sequencing assay of the microbial community for the three feeding strategies indicated that although the feeding strategy impacted reactor performance, it did not significantly alter the microbial community. The bacteria community composition maintained a similar degree of diversity. Proteobacteria, Bacteroidetes, and Verrucomicrobia were the dominant bacterial phyla in the system. Dominant PAOs were from the class Betaproteobacteria and the genera Paracoccus and Thauera. Glycogen-accumulating organisms were significantly inhibited while other less-known bacteria such as Wandonia and Hyphomonas were observed in all three reactors.

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Section: Distribution Of the Microbial Profilementioning

confidence: 99%

Phosphorus Removal from Aerobic Granular Sludge: Proliferation of Polyphosphate-Accumulating Organisms (PAOs) under Different Feeding Strategies

et al. 2022

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“…(A2) expressed greater aerobic nitrate removal capacity similar to numerous aerobic bacteria. For instance, Paracoccus denitrifcans strain removed 90.00% of NO 3 --N in a 250 mg/L initial NO 3 --N medium (Medhi et al, 2018). Similar aerobic nitrate removal (87.63%) was achieved by Paracoccus denitrifcans strain Z195 (Zhang et al, 2020).…”

Section: Optimization Of the Percentage Of The Carbon Sources For Aer...mentioning

confidence: 71%

Screening of potential aerobic denitrifying bacteria for nitrate removal from water

Kirisan¹,

Gnanavelrajah²,

Ratnayake³

2022

J. Natn. Sci. Foundation Sri Lanka

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Nitrate pollution in groundwater is a common problem in areas where inorganic fertilizer is used to a large extent. This situation seriously affects communities that use ground water as their main source of drinking water and for many other purposes. Therefore, finding an efficient and cost effective system for the removal of nitrate from groundwater is an urgent necessity. The present study was aimed at identifying aerobic bacteria isolated from various soils and water sources and to test their potential for reducing nitrate in groundwater. The bacterial isolates (n = 128) were screened for nitrate reduction by various processes in nutrient broth and in mineral salt medium containing glucose and starch, using KNO 3 as the nitrate substrate. Liberated gases during nitrate reduction were analyzed using gas chromatography. Out of 128 morphologically different isolates, two strains, namely Paracoccus sp. (A2) and Bacillus sp. (A19), were selected for further analysis on the basis of their performance for water treatment. The nitrate reduction percentages of A2 and A19 were within the range of 59.63-100% and 86.67-100%, respectively. Gas chromatography results indicated that these two strains liberated a higher percentage of N 2 (68 -90%) compared to N 2 O (5-13%) and CO 2 (traces) , while reducing the amount of nitrate. These results confirmed that A2 and A19 have the potential to be used in bioremediation of nitrate contaminated groundwater.

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“…Microorganisms with high betweenness centrality often lie on the shortest path between two other nodes, and for this reason, they are considered important for the flow of information between community members 30 . One of these key taxa in the biofilm network was Paracoccus , which is a heterotrophic nitrifying and aerobic denitrifying bacterium that can also remove phosphorus 31 . Other groups of bacteria with high betweenness centrality were Pseudoxanthomonas (capable of removing nitrogen and phosphorous under aerobic conditions 32 and Phaeodactylibacter (a denitrifier 33 ).…”

Section: Discussionmentioning

confidence: 99%

Development of microbial communities in biofilm and activated sludge in a hybrid reactor

Godzieba

Żubrowska-Sudoł

Walczak

et al. 2022

Sci Rep

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Microorganisms play a key role in biological wastewater treatment. The form in which biomass develops determines the efficiency and mechanisms of organic compound conversion, due to different conditions in various microbial structures. However, the results of studies comparing the microbial communities in biofilm and activated sludge have often conflicted. Therefore, this study compared the composition and development of the bacterial communities in biofilm and activated sludge in a hybrid reactor, employing 16S rRNA sequencing. Statistical analysis of the sequencing data included the identification of taxa characteristic to the biofilm and activated sludge, alpha and beta diversity analysis, and network analysis. These analyses indicated that the biofilm bacterial community was richer and more diverse than the activated sludge community. The mean numbers of OTU were 1614 in the biofilm and 993 in the activated sludge, and the mean values of the Chao1 (1735 vs. 1105) and Shannon (5.3 vs. 4.3) biodiversity indices were significantly higher for the biofilm. The biofilm was a better environment for development of nitrifiers (e.g., Nitrosomonas, Nitrospira) and phosphorus accumulating organisms (Candidatus Accumulibacter). Bacteria in the biofilm co-occurrence network had more connections (based on Spearman's rank correlation coefficient) with each other, indicating that they interact more than those in the activated sludge.

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Genome Sequence of a Heterotrophic Nitrifier and Aerobic Denitrifier, Paracoccus denitrificans Strain ISTOD1, Isolated from Wastewater

Cited by 12 publications

References 10 publications

Phosphorus Removal from Aerobic Granular Sludge: Proliferation of Polyphosphate-Accumulating Organisms (PAOs) under Different Feeding Strategies

Phosphorus Removal from Aerobic Granular Sludge: Proliferation of Polyphosphate-Accumulating Organisms (PAOs) under Different Feeding Strategies

Screening of potential aerobic denitrifying bacteria for nitrate removal from water

Development of microbial communities in biofilm and activated sludge in a hybrid reactor

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