Genome Sequence of a Clinical Isolate of
            <i>Campylobacter jejuni</i>
            from Thailand

Poly, Frédéric; Read, Timothy D.; Tribble, David R.; Baqar, Shahida; Lorenzo, María; Guerry, Patricia

doi:10.1128/iai.00050-07

Cited by 69 publications

(30 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, mutation of fspA in 81-176 had no effect on invasion. Despite isolation from a dysentery case, strain CG8486 is noninvasive in vitro (38), so it is unlikely that FspA 8486 plays a role in invasion. Consistent with this, the transfer of fspA 8486 into 81-176 (strain PG2662) or into 81-176 fspA 81-176 (strain PG2663) did not affect adherence or invasion compared to the wild type (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…NZAANY01000003.1; locus tag CJJ81176_0875) encodes a predicted soluble, cytoplasmic protein of 15.5 kDa (pI 4.4). The Cj0859c gene from CG8486, a clinical isolate from Thailand (38), encodes a predicted soluble, cytoplasmic protein of 16.0 kDa (pI 5.62). These two variants are 41.6% identical at the protein level (Fig.…”

Section: Cj0859c Is Expressed Bymentioning

confidence: 99%

“…3C, but did not show the aberrant migration observed with FspA 81-176 . CG8486 is difficult to manipulate genetically (38). The fspA gene and its 28 promoter from CG8486 were inserted into the astA gene of 81-176 to generate PG2662.…”

Section: Cj0859c Is Expressed Bymentioning

confidence: 99%

See 2 more Smart Citations

Heterogeneity of a Campylobacter jejuni Protein That Is Secreted through the Flagellar Filament

et al. 2007

Self Cite

View full text Add to dashboard Cite

Cj0859c, or FspA, is a small, acidic protein ofCampylobacter jejunithat is expressed by a σ28promoter. Analysis of thefspAgene in 41 isolates ofC. jejunirevealed two overall variants of the predicted protein, FspA1 and FspA2. Secretion of FspA occurs in broth-grown bacteria and requires a minimum flagellar structure. The addition of recombinant FspA2, but not FspA1, to INT407 cells in vitro resulted in a rapid induction of apoptosis. These data define a novelC. jejunivirulence factor, and the observed heterogeneity amongfspAalleles suggests alternate virulence potential among different strains.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Cj0859c Is Expressed Bymentioning

confidence: 99%

See 1 more Smart Citation

Heterogeneity of a Campylobacter jejuni Protein That Is Secreted through the Flagellar Filament

et al. 2007

Self Cite

View full text Add to dashboard Cite

show abstract

“…For example, 106 genes differentially existed between C. jejuni NCTC 11168 and CG8486 (5), and the size of the pan-genome (the sum of all genes found in a species) of C. jejuni is estimated to be 2,600 genes (6). It has been proposed that horizontal gene transfer (HGT) plays a critical role in the diversity of bacterial genomes, therefore contributing to bacterial evolution and adaptation in different environmental niches.…”

mentioning

confidence: 99%

Heat Shock-Enhanced Conjugation Efficiency in Standard Campylobacter jejuni Strains

Zeng

Ardeshna

Lin

2015

Appl Environ Microbiol

View full text Add to dashboard Cite

Campylobacter jejuni, the leading bacterial cause of human gastroenteritis in the United States, displays significant strain diversity due to horizontal gene transfer. Conjugation is an important horizontal gene transfer mechanism contributing to the evolution of bacterial pathogenesis and antimicrobial resistance. It has been observed that heat shock could increase transformation efficiency in some bacteria. In this study, the effect of heat shock on C. jejuni conjugation efficiency and the underlying mechanisms were examined. With a modified Escherichia coli donor strain, different C. jejuni recipient strains displayed significant variation in conjugation efficiency ranging from 6.2 ؋ 10 ؊8 to 6.0 ؋ 10 ؊3 CFU per recipient cell. Despite reduced viability, heat shock of standard C. jejuni NCTC 11168 and 81-176 strains (e.g., 48 to 54°C for 30 to 60 min) could dramatically enhance C. jejuni conjugation efficiency up to 1,000-fold. The phenotype of the heat shock-enhanced conjugation in C. jejuni recipient cells could be sustained for at least 9 h. Filtered supernatant from the heat shock-treated C. jejuni cells could not enhance conjugation efficiency, which suggests that the enhanced conjugation efficiency is independent of secreted substances. Mutagenesis analysis indicated that the clustered regularly interspaced short palindromic repeats system and the selected restriction-modification systems (Cj0030/Cj0031, Cj0139/Cj0140, Cj0690c, and HsdR) were dispensable for heat shock-enhanced conjugation in C. jejuni. Taking all results together, this study demonstrated a heat shock-enhanced conjugation efficiency in standard C. jejuni strains, leading to an optimized conjugation protocol for molecular manipulation of this organism. The findings from this study also represent a significant step toward elucidation of the molecular mechanism of conjugative gene transfer in C. jejuni.

show abstract

“…cgi) contains ongoing and completed genome sequence projects on nine different Campylobacter species (listed in table 1) [3,[6][7][8][9][10][11]. These include four complete genome sequences from different C. jejuni isolates (NCTC11168, RM1221, 81-176 and 81116), and several incomplete or unfinished C. jejuni sequences and sequences from other Campylobacter species.…”

Section: An Overview Of the Campylobacter Genomementioning

confidence: 99%

Genomics of Thermophilic <i>Campylobacter</i> Species

Gaskin¹,

Reuter²,

Shearer³

et al. 2009

Microbial Pathogenomics

View full text Add to dashboard Cite

The thermophilic Campylobacter species C. jejuni and C. coli are important human pathogens, which are major causes of bacterial gastroenteritis. The recent progress in genomics techniques has allowed for a rapid increase in our knowledge of the molecular biology of Campylobacter species, but needs to be matched by concurrent increases in our understanding of the unique biology of these organisms. Campylobacter species display significant levels of genomic variation via natural transformation, phase variation, plasmid transfer and infection with bacteriophages, and this poses a continuous challenge for studies on pathogenesis, physiology, epidemiology and evolution of Campylobacter. In this chapter we will review the current state of the art of the genomics of thermophilic Campylobacter species, and opportunities where genomics can further contribute to our understanding of the biology of these successful human pathogens.

show abstract

Genome Sequence of a Clinical Isolate of Campylobacter jejuni from Thailand

Cited by 69 publications

References 44 publications

Heterogeneity of a Campylobacter jejuni Protein That Is Secreted through the Flagellar Filament

Heterogeneity of a Campylobacter jejuni Protein That Is Secreted through the Flagellar Filament

Heat Shock-Enhanced Conjugation Efficiency in Standard Campylobacter jejuni Strains

Genomics of Thermophilic <i>Campylobacter</i> Species

Contact Info

Product

Resources

About