Genome sequence and transcriptome profiles of pathogenic fungus Paecilomyces penicillatus reveal its interactions with edible fungus Morchella importuna

Cheng, Chen; Fu, Rongtao; Wang, Jian; Li, Xingyue; Chen, Xiaojuan; Li, Qiang; Lu, Dalei

doi:10.1016/j.csbj.2021.04.065

Cited by 13 publications

(11 citation statements)

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“…With the expansion of cultivation range and density of morel production, disease have become the main factor limiting its yield. Pileus rot disease (He et al 2018a ), stipe rot disease (Guo et al 2016 ), white mold disease (He et al 2017 ; Wang et al 2020 ; Chen et al 2021 ), and cobweb disease (Lan et al 2020 ) are currently considered to be the four most serious diseases in morel industry (Fig. 19 ).…”

Section: Growing Morels In Chinamentioning

confidence: 99%

“…Dual culture assays showed that certain contact-independent soluble compounds secreted by P. penicillatus can inhibit the mycelial growth of M. sextelata (Wang et al 2020 ). Genomics data demonstrated that P. penicillatus encodes a large number of fungal cell wall degradation enzymes (Wang et al 2020 ), and transcriptome analysis showed that the genes involved in diphthamide biosynthesis, aldehyde reductase, and NAD (P)H-hydrate epimerase in P. penicillatus were up-regulated during the process of this fungus infection of M. importuna (Chen et al 2021 ).…”

Section: Growing Morels In Chinamentioning

confidence: 99%

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Ten decadal advances in fungal biology leading towards human well-being

et al. 2022

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Fungi are an understudied resource possessing huge potential for developing products that can greatly improve human well-being. In the current paper, we highlight some important discoveries and developments in applied mycology and interdisciplinary Life Science research. These examples concern recently introduced drugs for the treatment of infections and neurological diseases; application of –OMICS techniques and genetic tools in medical mycology and the regulation of mycotoxin production; as well as some highlights of mushroom cultivaton in Asia. Examples for new diagnostic tools in medical mycology and the exploitation of new candidates for therapeutic drugs, are also given. In addition, two entries illustrating the latest developments in the use of fungi for biodegradation and fungal biomaterial production are provided. Some other areas where there have been and/or will be significant developments are also included. It is our hope that this paper will help realise the importance of fungi as a potential industrial resource and see the next two decades bring forward many new fungal and fungus-derived products.

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Section: Growing Morels In Chinamentioning

confidence: 99%

Section: Growing Morels In Chinamentioning

confidence: 99%

Ten decadal advances in fungal biology leading towards human well-being

et al. 2022

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“…P. penicillatus was found to harbor many CAZymes, particularly clusters of chitinase genes, which could be related to its pathogenicity ( Wang et al, 2020 ). Cheng et al (2021) reported the regulatory mechanism of interaction between P. penicillatus and M. importuna . However, the lack of a control group of M. importuna at the same developmental stage complicated the conclusions from that study.…”

Section: Introductionmentioning

confidence: 99%

Dual RNA-Seq Analysis of the Interaction Between Edible Fungus Morchella sextelata and Its Pathogenic Fungus Paecilomyces penicillatus Uncovers the Candidate Defense and Pathogenic Factors

Tan

Liu

et al. 2021

Front. Microbiol.

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Morels (Morchella spp.) are economically important mushrooms cultivated in many countries. However, their production and quality are hindered by white mold disease because of Paecilomyces penicillatus infection. In this study, we aimed to understand the genetic mechanisms of interactions between P. penicillatus and Morchella. M. sextelata, the most prevalent species of Morchella in China, was inoculated with P. penicillatus; then, the expression profiles of both fungi were determined simultaneously at 3 and 6 days post-inoculation (dpi) using a dual RNA-Seq approach. A total of 460 and 313 differentially expressed genes (DEGs) were identified in P. penicillatus and M. sextelata, respectively. The CAZymes of β-glucanases and mannanases, as well as subtilase family, were upregulated in P. penicillatus, which might be involved in the degradation of M. sextelata cell walls. Chitin recognition protein, caffeine-induced death protein, and putative apoptosis-inducing protein were upregulated, while cyclin was downregulated in infected M. sextelata. This indicates that P. penicillatus could trigger programmed cell death in M. sextelata after infection. Laccase-2, tyrosinases, and cytochrome P450s were also upregulated in M. sextelata. The increased expression levels of these genes suggest that M. sextelata could detoxify the P. penicillatus toxins and also form a melanin barrier against P. penicillatus invasion. The potential pathogenic mechanisms of P. penicillatus on M. sextelata and the defense mechanisms of M. sextelata against P. penicillatus were well described.

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“…In group III, the relative abundance of those pathogenic fungi, such as Gibberella , Microidium , Penicillium , Sarocladium and Streptomyces , and Trichoderma , may be the main cause of low or no yield of morels. At present, Cladobotryum spp., Diploöspora longispora , Fusarium spp., and Paecilomyces penicillatus were considered as the four most serious disease-causing fungi of the morel industry [ 16 , 17 , 18 , 19 , 71 ]. However, how many potential pathogenic fungi are there?…”

Section: Discussionmentioning

confidence: 99%

Morel Production Associated with Soil Nitrogen-Fixing and Nitrifying Microorganisms

Jayawardena

Thongklang

et al. 2022

JoF

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True morels (Morchella, Pezizales) cultivated in soil are subject to complex influences from soil microbial communities. To explore the characteristics of soil microbial communities on morel cultivation, and evaluate whether these microbes are related to morel production, we collected 23 soil samples from four counties in Sichuan and Yunnan Provinces, China. Based on ITS and 16S rDNA amplicon sequencing, the alpha diversity analysis indicated that the biodiversity of morel cultivation soil showed a downward trend compared with the bare soil. The results also showed that there were no significant differences in soil microbial communities between OC (bare soil) and OO (after one-year suspension of sowing). This means that, after about one year of stopping sowing, the component and structure of soil that once cultivated morel would be restored. In co-occurrence networks, some noteworthy bacterial microbes involved in nitrogen fixation and nitrification have been identified in soils with high morel yields, such as Arthrobacter, Bradyhizobium, Devosia, Pseudarthrobacter, Pseudolabrys, and Nitrospira. In contrast, in soils with low or no morel yield, some pathogenic fungi accounted for a high proportion, including Gibberella, Microidium, Penicillium, Sarocladium, Streptomyces, and Trichoderma. This study provided valuable information for the isolation and culturing of some beneficial microbes for morel cultivation in further study and, potentially, to harness the power of the microbiome to improve morel production and health.

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Genome sequence and transcriptome profiles of pathogenic fungus Paecilomyces penicillatus reveal its interactions with edible fungus Morchella importuna

Cited by 13 publications

References 78 publications

Ten decadal advances in fungal biology leading towards human well-being

Ten decadal advances in fungal biology leading towards human well-being

Dual RNA-Seq Analysis of the Interaction Between Edible Fungus Morchella sextelata and Its Pathogenic Fungus Paecilomyces penicillatus Uncovers the Candidate Defense and Pathogenic Factors

Morel Production Associated with Soil Nitrogen-Fixing and Nitrifying Microorganisms

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