Genome sequence and organization of a nucleopolyhedrovirus that infects the tea looper caterpillar, Ectropis obliqua

Abstract

The complete nucleotide sequence of Ectropis obliqua nucleopolyhedrovirus (EcobNPV), which infects the tea looper caterpillar, was determined and analyzed. The double stranded circular genome is composed of 131,204 bp and is 37.6% G+C rich. The analysis predicted 126 putative, minimally overlapping open reading frames (ORFs) with 150 or more nucleotides that together compose 89.8% of the genome. The remaining 10.2% constitute non-coding and three homologous regions. Comparison with previously sequenced baculoviruses indicated that three ORFs were unique to EcobNPV, while the remaining 123 ORFs shared identity with other baculovirus genes. In addition to two bro homologues, three other repeat ORFs, including dbp, p26, and odv-e66, were identified. Phylogenetic analysis indicated that each member of the paired ORFs was acquired independently. Gene parity plot analysis and percent identity of gene homologues suggested that EcobNPV is a Group II NPV, although its genomic organization was highly distinct.

Morphology and genome of Euproctis pseudoconspersa nucleopolyhedrovirus

“…The repeat sequence among each hrs was conserved. When compared to EcobNPV hrs, neither the nucleotide sequence nor the location on the genome, the EupsNPV hrs have obvious similarity with EcobNPV hrs, except that the 'F' sequence of EupsNPV hr4 had 80% identity with EcobNPV hrs [9].…”

“…NC_001623 [8], E. obliqua SNPV (EcobNPV; GenBank accession no. DQ837165 [9], L. dispar MNPV (Ld MNPV; GenBank accession no. NC_001973 [10], S. exigua MNPV (SeMNPV; GenBank accession no.…”

Genomic sequence analysis of the Illinois strain of the Agrotis ipsilon multiple nucleopolyhedrovirus

“…Parameters for alignment and phylogenetic inference were as previously described [18]. Taxa analyzed included group II NPVs with completely sequenced genomes [14,18,[21][22][23][24][25][26][27][28][29][30][31][32][33][34][35], AcMNPV-C6 [36], and Cydia pomonella GV [37].…”

The Genome of Pieris rapae Granulovirus

“…Genomic DNA was extracted from the purified occlusion bodies. The entire genome of PrGV was sequenced, assembled, and analyzed as previously reported (4).…”

“…Moreover, the PrGV genome contained eight typical homologous regions and two repeated genes (orf39/44 and ODV-E66a/b) dispersed at different loci within the genome. Two odv-e66 homologues were also identified in the Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) (3) and Ectropis obliqua nucleopolyhedrovirus (EoNPV) genomes (4).…”