Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus

Ongenae, Véronique; Azeredo, Joana; Kropinski, Andrew M.; Rozen, Daniel E.; Briegel, Ariane; Claessen, Dennis

doi:10.21203/rs.3.rs-1784137/v1

Cited by 2 publications

(3 citation statements)

References 13 publications

(13 reference statements)

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“…For the discovery and mechanistic understanding of novel defense strategies a diverse set of phages is needed incentivising the isolation and characterization of new phages. Using different Streptomyces species as isolation host, several new phages have been isolated and described in recent years 18–20 . These studies show that the common notion of a narrow host range and specific infection of phages does not necessarily apply to Streptomyces phages, as several actinophages have been described to have a broader host range and to productively infect several species of the genus 20,21 .…”

Section: Introductionmentioning

confidence: 99%

“…Using different Streptomyces species as isolation host, several new phages have been isolated and described in recent years 18–20 . These studies show that the common notion of a narrow host range and specific infection of phages does not necessarily apply to Streptomyces phages, as several actinophages have been described to have a broader host range and to productively infect several species of the genus 20,21 . However, in most studies only a small number of different host species has been tested delimitating the information regarding host range.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of the newStreptomycesphages Kamino, Geonosis, Abafar and Scarif infecting a broad range of host species

Rackow,

Rolland,

Mohnen

et al. 2024

Preprint

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Streptomyces, a multifaceted genus of soil-dwelling bacteria within the phylum Actinobacteria, features intricate phage-host interactions shaped by its complex life cycle and the synthesis of a diverse array of specialized metabolites. Here, we describe the isolation and characterization of four novelStreptomycesphages infecting a variety of different host species. While phage Kamino, isolated onStreptomyces kasugaensis, is predicted to be temperate and encodes a serine integrase in its genome, phages Geonosis (isolated onS. griseus) and phages Abafar and Scarif, isolated onS. albidoflavus, are virulent phages. Phages Kamino and Geonosis were shown to amplify well in liquid culture leading to a pronounced culture collapse already at low titres. Determination of the host range by testing >40 differentStreptomycesspecies identified phages Kamino, Abafar and Scarif as broad host range phages, with Kamino showing productive infections on 15 different species. Isolated phages were further tested regarding their sensitivity to antiphage molecules. Here, the strongest effects were observed for the DNA-intercalating molecule daunorubicin. Overall, the phages described in this study expand the publicly available portfolio of phages infectingStreptomycesand will be instrumental for advancing the mechanistic understanding of the intricate antiviral strategies employed by these multicellular bacteria.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of the newStreptomycesphages Kamino, Geonosis, Abafar and Scarif infecting a broad range of host species

Rackow,

Rolland,

Mohnen

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Isolation and characterization of the new Streptomyces phages Kamino, Geonosis, Abafar, and Scarif infecting a broad range of host species

Rackow,

Rolland,

Mohnen

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

Streptomyces , a multifaceted genus of soil-dwelling bacteria belonging to the phylum Actinomycetota , features intricate phage-host interactions shaped by its complex life cycle and the synthesis of a diverse array of specialized metabolites. Here, we describe the isolation and characterization of four novel Streptomyces phages infecting a variety of different host species. While phage Kamino, isolated on Streptomyces kasugaensis , is predicted to be temperate and encodes a serine integrase in its genome, phages Geonosis (isolated on Streptomyces griseus ) and Abafar and Scarif, isolated on Streptomyces albidoflavus , are virulent phages. Phages Kamino and Geonosis were shown to amplify well in liquid culture leading to a pronounced culture collapse already at low titers. Determination of the host range by testing >40 different Streptomyces species identified phages Kamino, Abafar, and Scarif as broad host-range phages. Overall, the phages described in this study expand the publicly available portfolio of phages infecting Streptomyces and will be instrumental in advancing the mechanistic understanding of the intricate antiviral strategies employed by these multicellular bacteria. IMPORTANCE The actinobacterial genus Streptomyces is characterized by multicellular, filamentous growth and the synthesis of a diverse range of bioactive molecules. These characteristics also play a role in shaping their interactions with the most abundant predator in the environment, bacteriophages—viruses infecting bacteria. In this study, we characterize four new phages infecting Streptomyces . Out of those, three phages feature a broad host range infecting up to 15 different species. The isolated phages were characterized with respect to plaque and virion morphology, host range, and amplification in liquid culture. In summary, the phages reported in this study contribute to the broader collection of publicly available phages infecting Streptomyces , playing a crucial role in advancing our mechanistic understanding of phage-host interactions of these multicellular bacteria.

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Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus

Cited by 2 publications

References 13 publications

Isolation and characterization of the newStreptomycesphages Kamino, Geonosis, Abafar and Scarif infecting a broad range of host species

Isolation and characterization of the newStreptomycesphages Kamino, Geonosis, Abafar and Scarif infecting a broad range of host species

Isolation and characterization of the new Streptomyces phages Kamino, Geonosis, Abafar, and Scarif infecting a broad range of host species

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