Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus

Ongenae, Véronique; Azeredo, Joana; Kropinski, Andrew M.; Rozen, Daniel E.; Briegel, Ariane; Claessen, Dennis

doi:10.1038/s41598-022-22784-y

Cited by 7 publications

(10 citation statements)

References 23 publications

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“…Since the full nucleotide sequence was not similar to any phages, we performed phylogenetic analysis on the major capsid protein (GenBank: WAX22437.1), which is identified in most phage genomes and has been used previously for phylogenetic analysis 20 , 21 . There were 62 additional phage proteins with high percent amino acid identity to the major capsid protein encoded by MiCath.…”

Section: Resultsmentioning

confidence: 99%

Genome sequence and characterization of a novel Pseudomonas putida phage, MiCath

Jaryenneh,

Schoeniger,

Mageeney

2023

Sci Rep

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Pseudomonads are ubiquitous bacteria with importance in medicine, soil, agriculture, and biomanufacturing. We report a novel Pseudomonas putida phage, MiCath, which is the first known phage infecting P. putida S12, a strain increasingly used as a synthetic biology chassis. MiCath was isolated from garden soil under a tomato plant using P. putida S12 as a host and was also found to infect four other P. putida strains. MiCath has a ~ 61 kbp double-stranded DNA genome which encodes 97 predicted open reading frames (ORFs); functions could only be predicted for 48 ORFs using comparative genomics. Functions include structural phage proteins, other common phage proteins (e.g., terminase), a queuosine gene cassette, a cas4 exonuclease, and an endosialidase. Restriction digestion analysis suggests the queuosine gene cassette encodes a pathway capable of modification of guanine residues. When compared to other phage genomes, MiCath shares at most 74% nucleotide identity over 2% of the genome with any sequenced phage. Overall, MiCath is a novel phage with no close relatives, encoding many unique gene products.

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Section: Resultsmentioning

confidence: 99%

Genome sequence and characterization of a novel Pseudomonas putida phage, MiCath

Jaryenneh,

Schoeniger,

Mageeney

2023

Sci Rep

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“…The one-step growth curve of Vanseggelen and Verabelle showed a long latent period of 140 minutes and a relatively low burst size, even when compared to other Streptomyces phages. 5,21 The low burst size could be the result of the morphological complexity of Streptomyces 21 . The meaning of MOI loses its signi cance once spores have germinated and a mycelium is formed as multiple phages are able to attach, but the mycelium would still be counted as one colony forming unit 13,22 .…”

Section: Discussionmentioning

confidence: 99%

“…Streptomyces phages Vanseggelen and Verabelle were isolated from a soil sample obtained from the National Park Zuid-Kennemerland in the Netherlands (N52°23′31″, E4°34′49″) using Streptomyces coelicolor as the host. Details on isolation procedures are reported previously in detail 5 . The collected phage stock solutions were stored at 4°C.…”

Section: Bacteriophage Isolationmentioning

confidence: 99%

“…Bacteriophages, or phages for short, are viruses that can infect bacteria. Yet, it remains largely unknown how bacteriophages can recognize, attach and infect these multicellular bacteria 5 . All known Streptomyces phages belong to the class Caudoviricetes, which are tailed bacterial and archaeal viruses with icosahedral capsid heads and double-stranded DNA genomes 6 .…”

Section: Introductionmentioning

confidence: 99%

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Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus

Ongenae,

Kempff,

Neer

et al. 2023

Preprint

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Despite the rising interest in bacteriophages, little is known about their infection cycle and lifestyle in a multicellular host. Even in the model system Streptomyces, only a small number of phages have been sequenced and well characterized so far. Here, we report the complete characterization and genome sequences of Streptomyces phages Vanseggelen and Verabelle isolated fromStreptomyces coelicolor. A wide range of Streptomyces strains could be infected by both phages, but neither of the two phages was able to infect members of the closely related sister genus Kitasatospora. The phages Vanseggelen and Verabelle have a double-stranded DNA genome with lengths of 48,720 and 48,126 bp, respectively. Both phage genomes contain 72 putative genes, and the presence of an integrase encoding protein indicates a lysogenic lifestyle. Characterization of the phages revealed their stability over a wide range of temperatures (25–45°C) and pH values (4–10). In conclusion, Streptomyces phage Vanseggelen and Streptomyces phage Verabelle are newly isolated phages that can be classified as new species in the genus Camvirus, within the subfamily Arquattrovirinae.

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“…The last few years have been marked by a sustained effort in the isolation and sequencing of Streptomyces phages from environmental samples (17–19, 10, 20, 21). Moreover, the Streptomyces genome can constitute per se a viral genetic reservoir, through the genomes of temperate prophages that they may host.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novel temperate phage facilitatingin vitrodispersal of multicellular bacteria

Jaffal,

Kortebi,

Misson

et al. 2023

Preprint

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Streptomycesare renowned for their prolific production of specialized metabolites with applications in medicine and agriculture. These multicellular bacteria present a sophisticated developmental cycle, and play a key role in soil ecology. Little is known aboutStreptomyces-phage interactions and the impact of phages onStreptomycesphysiology. In this study, we investigated the conditions governing the expression and production of ′Samy ′, a prophage found inStreptomyces ambofaciensATCC 23877. This phage is a siphovirus produced simultaneously with the activation of other mobile genetic elements. We show that Samy production increases bacterial dispersal in stress conditions, facilitating the dissemination of the lineage. Altogether, we unveiled a new property of a bacteriophage infection that it is closely linked to the multicellular community life ofStreptomycesbacteria.

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Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus

Cited by 7 publications

References 23 publications

Genome sequence and characterization of a novel Pseudomonas putida phage, MiCath

Genome sequence and characterization of a novel Pseudomonas putida phage, MiCath

Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus

Characterization of a novel temperate phage facilitatingin vitrodispersal of multicellular bacteria

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