Genome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil

Abstract

A deep-sea fungus Aspergillus sydowii BOBA1 isolated from marine sediment at a depth of 3000 m was capable of degrading spent engine (SE) oil. The response of immobilized fungi towards degradation at elevated pressure was studied in customized high pressure reactors without any deviation in simulating in situ deep-sea conditions. The growth rate of A. sydowii BOBA1 in 0.1 MPa was significantly different from the growth at 10 MPa pressure. The degradation percentage reached 71.2 and 82.5% at atmospheric and high pressure conditions, respectively, within a retention period of 21 days. The complete genome sequence of BOBA1 consists of 38,795,664 bp in size, comprises 2582 scaffolds with predicted total coding genes of 18,932. A total of 16,247 genes were assigned with known functions and many families found to have a potential role in PAHs and xenobiotic compound metabolism. Functional genes controlling the pathways of hydrocarbon and xenobiotics compound degrading enzymes such as dioxygenase, decarboxylase, hydrolase, reductase and peroxidase were identified. The spectroscopic and genomic analysis revealed the presence of combined catechol, gentisate and phthalic acid degradation pathway. These results of degradation and genomic studies evidenced that this deep-sea fungus could be employed to develop an eco-friendly mycoremediation technology to combat the oil polluted marine environment. This study expands our knowledge on piezophilic fungi and offer insight into possibilities about the fate of SE oil in deep-sea.

Insight into the adaptation mechanisms of high hydrostatic pressure in physiology and metabolism of hadal fungi from the deepest ocean sediment

“…Early studies on culturable filamentous fungi, to our knowledge, were mostly under mild HHP ( 17 , 60 ), and very few of them exceed 30 MPa ( 9 , 18 , 32 ). Considering the truth that conidia and hyphae showed serious growth defects under 60 MPa in our preliminary experiment, a compromised pressure of up to 40 MPa was ultimately employed in our study.…”

“…More and more deep-sea fungi and their potential roles, such as the ecological functions and novel secondary metabolites productions ( 3 , 9 , 58 ), were discovered from various depths in the deep sea. However, apart from some prokaryotes and yeast species ( 10 , 13 , 59 ), limited information is available for adaptation and genome informatics of culturable filamentous fungi under HHP, and relatively few studies paid attention to the relationship between their physiological property and HHP ( 13 , 14 , 17 ). In our work, we used three homogeneous A. sydowii as the representative to explore the piezotolerance of filamentous fungi.…”

“…Filamentous fungi Aspergillus spp. have demonstrated good secondary metabolic activity and potential to degrade the xenobiotic compounds under elevated HHP ( 3 , 17 ). Deng et al have reported that HHP regulated the production of fungal secondary metabolites ( 18 ).…”

Whole-Genome Sequencing and Comparative Genomic Analysis of Potential Biotechnological Strains of Trichoderma harzianum, Trichoderma atroviride, andTrichoderma reesei

“…The phenotypic and ecological heterogeneity across fungi of the same genera is reflected, in part, by the diversity observed within their genomes (Priest et al, 2020). In this way, the diverse and important roles of fungi, as well as the technological advances in next-generation sequencing, have motivated broad efforts to sequence several fungal genomes (Ganesh Kumar et al, 2021; Hagestad et al, 2021; Nagel et al, 2021; Varga et al, 2019; Wu et al, 2018). Since the genome of T. reesei QM6a was first presented (Martinez et al, 2008), Trichoderma sequencing studies have increased with the goal of better understanding the biological and ecological roles of Trichoderma to improve their applications (Druzhinina et al, 2018b; Horta et al, 2014; Kubicek et al, 2011; Kubicek et al, 2019; Li et al, 2017; Schmoll et al, 2016).…”

“…The phenotypic and ecological heterogeneity across fungi of the same genera is reflected, in part, by the diversity observed within their genomes (Priest et al, 2020). In this way, the diverse and important roles of fungi, as well as the technological advances in next-generation sequencing, have motivated broad efforts to sequence several fungal genomes (Hagestad et al, 2021;Kumar et al, 2021;Nagel et al, 2021;Varga et al, 2019;Wu et al, 2018).…”