Genome-scale exploration of transcriptional regulation in the nisin Z producer Lactococcus lactis subsp. lactis IO-1

Abstract

Transcription is of the most crucial steps of gene expression in bacteria, whose regulation guarantees the bacteria's ability to adapt to varying environmental conditions. Discovering the molecular basis and genomic principles of the transcriptional regulation is thus one of the most important tasks in cellular and molecular biology. Here, a comprehensive phylogenetic footprinting framework was implemented to predict maximal regulons of Lactococcus lactis subsp. lactis IO-1, a lactic acid bacterium known for its high potentials in nisin Z production as well as efficient xylose consumption which have made it a promising biotechnological strain. A total set of 321 regulons covering more than 90% of all the bacterium's operons have been elucidated and validated according to available data. Multiple novel biologically-relevant members were introduced amongst which arsC, mtlA and mtl operon for BusR, MtlR and XylR regulons can be named, respectively. Moreover, the effect of riboflavin on nisin biosynthesis was assessed in vitro and a negative correlation was observed. It is believed that understandings from such networks not only can be useful for studying transcriptional regulatory potentials of the target organism but also can be implemented in biotechnology to rationally design favorable production conditions.

Metabiotics Signature through Genome Sequencing and In Vitro Inhibitory Assessment of a Novel Lactococcus lactis Strain UTNCys6-1 Isolated from Amazonian Camu-Camu Fruits

“…The query sequence showed a conserved domain similar to nisin_A. Nisin Z differs from the typical nisin A peptide by only one amino acid, resulting in greater solubility at physiological pH, making it a more suitable candidate for biomedical applications [ 39 ]. Likewise, the nisin_Z class was found in L. lactis KF282 but not in L. lactis ATCC19435 or L. lactis ATCC11474 ( Figure 5 B).…”

Understanding the Effect of Different Glucose Concentrations in the Oligotrophic Bacterium Bacillus subtilis BS-G1 through Transcriptomics Analysis

“…Glucose serves as a primary carbon source for microorganisms, and prior empirical findings have demonstrated that the expression of seven crucial mRNAs related to carbohydrate transport is notably elevated under conditions of low glucose levels. Notably, the genes mtlA (gene0435) and celB (gene3876, gene3860) are prevalent across various strains, including Vibrio cholerae, Escherichia coli, Lactococcus lactis, Clavibacter michiganensis, and Listeria monocytogenes [105][106][107][108]. The subsequent research direction of our team involves utilizing mtlA (gene0435) and celB (gene3876 and gene3860) as bio-alert indicators to assess the nutrient status of soil based on their expression.…”

Adaptive laboratory evolution to hypersaline conditions of lactic acid bacteria isolated from seaweed

“…Under stress conditions LAB use the malolactic fermentation pathway where the CO 2 produced is transformed into bicarbonate by carbonic anhydrases to increase the intracellular pH [39]. Finally, ars operons are widely distributed in bacterial genomes, including LAB, such as Lactococcus lactic [48]. The protein ArsC is an enzyme reducing arsenate to arsenite, protecting the cell from high concentrations of arsenic [49].…”