Genome Resource of Two Potato Strains of <i>Ralstonia solanacearum</i> Biovar 2 (Phylotype IIB Sequevar 1) and Biovar 2T (Phylotype IIB Sequevar 25) Isolated from Lowlands in Iran

Sedighian, Nasim; Krijger, M.C.; Taparia, Tanvi; Taghavi, Seyed Mohsen; Wicker, Emmanuel; Wolf, J.M. van der; Osdaghi, Ebrahim

doi:10.1094/mpmi-02-20-0026-a

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Effective diagnostic tests capable of distinguishing between target and non‐target samples are needed to support regulative rules with some degree of confidence (Pritchard et al, 2016). Trade of solanaceous vegetables/crops is under strict control in Iran especially when it comes to transportation of seed lots (Ansari et al, 2019; Khojasteh et al, 2019; Osdaghi et al, 2022; Sedighian, Krijger, et al, 2020; Sedighian, Taghavi, et al, 2020). Rapid and accurate identification of seedborne bacteria is a critical issue in terms of both storage and international trade (Cigna et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Field surveys indicate taxonomically diverse Pectobacterium species inducing soft rot of vegetables and annual crops in Iran

Sattar

Osdaghi

2023

Plant Pathology

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Soft rot‐inducing bacterial pathogens are considered among the main biotic constraints on vegetables and annual crops. Most of the bacterial soft rot pathogens are members of Pectobacterium spp. and Dickeya, with the former genus encompassing a continuously increasing number of species varying in geographic distribution and host of isolation. In Iran, Pectobacterium species have rarely been investigated using high‐throughput nucleotide sequence‐based techniques, leaving the genetic diversity of these pathogens mostly undetermined. During 2021, vegetables and annual crops with symptoms of soft rot and maceration were collected from Fars, Hamedan, Isfahan, Khuzestan and Kohgiluyeh‐Boyer‐Ahmad provinces of Iran. Forty‐four pectinolytic bacterial strains were isolated from symptomatic tissues of bell pepper, cabbage, carrot, lettuce, potato and zucchini. The bacterial strains were subjected to pathogenicity and host range assays, specific PCR tests and phylogenetic analysis of three housekeeping genes (dnaX, gapA and leuS). All strains were pathogenic on their host of isolation. Among the 44 pectinolytic strains, 26 strains were identified as members of Pectobacterium based on PCR using the primer pair Y1/Y2. Multilocus sequence analysis confirmed the latter bacterial strains belonging to the genus Pectobacterium and revealed that the strains scattered into four species: P. brasiliense (five strains), P. carotovorum (eight strains), P. polaris (five strains) and P. versatile (eight strains). P. polaris and P. versatile have not previously been reported in the country. Host range assays showed that P. brasiliense and P. carotovorum have a broader host range than the other two species.

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Section: Discussionmentioning

confidence: 99%

Field surveys indicate taxonomically diverse Pectobacterium species inducing soft rot of vegetables and annual crops in Iran

Sattar

Osdaghi

2023

Plant Pathology

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“…The outbreaks caused by new isolates of the late blight oomycete Phytophthora infestans (Mont.) de Bary in Europe and the Americas ( Santana et al., 2013 ; Göre et al., 2019 ; Maurice et al., 2019 ), or by the new sequevars of bacterial wilt Ralstonia solanacearum found in Brazil, Peru and Iran ( Gutarra et al., 2017 ; Santiago et al., 2017 ; Sedighian et al., 2020 ) have the potential to destroy potato fields. Lack of resistance of the current potato cultivars to the main diseases that affect the crop makes it one of the most dependent crops on agrochemical applications ( Vleeshouwers et al., 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Solanum malmeanum, a promising wild relative for potato breeding

et al. 2023

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Crop wild relatives are gaining increasing attention. Their use in plant breeding is essential to broaden the genetic basis of crops as well as to meet industrial demands, for global food security and sustainable production. Solanum malmeanum (Solanum sect. Petota, Solanaceae) is a wild relative of potatoes (S. tuberosum) from Southern South America, occurring in Argentina, Brazil, Paraguay and Uruguay. This wild potato has been largely mistaken for or historically considered as conspecific with S. commersonii. Recently, it was reinstated at the species level. Retrieving information on its traits and applied uses is challenging, because the species name has not always been applied correctly and also because species circumscriptions and morphological criteria applied to recognize it have not been consistent. To overcome these difficulties, we performed a thorough literature reference survey, herbaria specimens’ identification revision and genebank database queries to review and update the information available on this potato wild relative, contributing to an increase in research on it to fully understand and explore its potential for potato breeding. Scarce studies have been carried out concerning its reproductive biology, resistance against pests and diseases as well as tolerance to abiotic stresses and evaluation of quality traits. The scattered information available makes it less represented in genebanks and genetic studies are missing. We compile, update and present available information for S. malmeanum on taxonomy, geographical distribution, ecology, reproductive biology, relationship with its closest relatives, biotic and abiotic stresses resistance and quality traits and discuss ways to overcome sexual barriers of hybridization and future perspectives for its use in potato breeding. As a final remark, we highlight that this species’ potential uses have been neglected and must be unlocked. Thus, further studies on morphological and genetic variability with molecular tools are fundamental for an efficient conservation and applied use of this promising genetic resource.

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Ralstonia solanacearum (bacterial wilt of potato)

Osdaghi¹

2022

CABI Compendium

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Genome Resource of Two Potato Strains of Ralstonia solanacearum Biovar 2 (Phylotype IIB Sequevar 1) and Biovar 2T (Phylotype IIB Sequevar 25) Isolated from Lowlands in Iran

Cited by 5 publications

References 16 publications

Field surveys indicate taxonomically diverse Pectobacterium species inducing soft rot of vegetables and annual crops in Iran

Field surveys indicate taxonomically diverse Pectobacterium species inducing soft rot of vegetables and annual crops in Iran

Solanum malmeanum, a promising wild relative for potato breeding

Ralstonia solanacearum (bacterial wilt of potato)

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