Genome relationships in polyploid<i>Poa pratensis</i>and other<i>Poa</i>species inferred from phylogenetic analysis of nuclear and chloroplast DNA sequences

Patterson, Jason T.; Larson, Steven R.; Johnson, Paul G.

doi:10.1139/g04-102

Cited by 46 publications

(86 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Observation of the occurrence of apomixis and the presence of “group segregation” in P. pratensis in Missouri, USA suggested an allopolyploid origin of the species [27]. Analysis of chloroplast and nuclear gene sequence data supports the idea that P. pratensis is, at least, partly allopolyploid [28]. In this study, P. pratensis from Qinghai, China, showed distinctly fewer Pp CR-1 and Pp TR-3 sites, compared with cultivars from the USA.…”

Section: Discussionmentioning

confidence: 95%

Isolation and characterization of chromosomal markers in Poa pratensis

Zhao

Liu

et al. 2017

Mol Cytogenet

View full text Add to dashboard Cite

Background Poa pratensis L. is a turf grass and forage crop used worldwide. Being a facultative apomictic species, P. pratensis has a highly variable chromosome number. Chromosomal markers constitute a powerful tool for chromosome identification and for various aspects of genomic research. However, currently, no chromosomal markers are available for P. pratensis.ResultsFour novel chromosome markers were isolated from a screen of Cot-1 DNA libraries, combined with fluorescence in situ hybridization (FISH) in Poa pratensis. Three tandemly repetitive sequences (PpTR-1, PpTR-2, and PpTR-3) were characterized as subtelomeric. Monomers of 318 bp, 189 bp and 189 bp were identified in PpTR-1, PpTR-2, and PpTR-3, respectively. One tandemly repetitive sequence (PpCR-1) was shown to be centromeric or pericentromeric, and it had a monomer of 27 bp. The distribution patterns of PpTR-1, PpTR-2, and PpTR-3 were highly conserved across different P. pratensis cultivars and in the distantly related Poa species, whereas PpCR-1 was conserved across different P. pratensis cultivars, but less conserved across Poa species.ConclusionIn this study, we report the identification and characterization of four novel chromosomal markers in P. pratensis. These chromosomal markers are powerful tools for accurate assessment of chromosome count, genomic and phylogenetic analyses, as well as studies of apomixis in P. pratensis. Electronic supplementary materialThe online version of this article (doi:10.1186/s13039-017-0307-7) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 95%

Isolation and characterization of chromosomal markers in Poa pratensis

Zhao

Liu

et al. 2017

Mol Cytogenet

View full text Add to dashboard Cite

show abstract

“…P. arachnifera, P. pratensis, and P. annua each produced multiple amplicons that were highly homologous to the KO orthologs in rice, barley, and wheat. The fact that these Poa species are polyploid may suggest that the multiple bands are gene copies from each genome rather than from a locus duplication event [20]. Poa annua however, produced four amplicons, two of which were most likely inherited from its ancestral diploid maternal parent P. infirma [21].…”

Section: Discussionmentioning

confidence: 99%

“…The gel electrophoresis also revealed an additional band in P. compressa that may be an allelic variant or an additional gene copy that is slightly larger than the upper P. arachnifera band ( Figure 2). P. compressa is also a polyploid, which could explain the additional gene copies [20]. The primer pair KO-F/TxComp-R was used to …”

Section: Discussionmentioning

confidence: 99%

Development of ent-kaurene Oxidase-Based Conserved Intron Spanning Primers for Species Identification in the Genus Poa (Poaceae; Bluegrass)

2018

View full text Add to dashboard Cite

Abstract:Interspecific hybridization has been attempted to combine the heat and drought of Poa arachnifera Torr. with the turf quality characteristics of several Poa species. Confirmation of an F 1 hybrid through morphological analysis of vegetative and flowering characteristics is often time consuming and ambiguous. Ent-kaurene oxidase (KO) has been sequenced in rice, barley, and wheat. In rice, each of the five copies of KO gene has unique lengths for the first intron. Conserved intron spanning primers (CISP) can be used as a DNA marker to exploit variations of intron lengths that flank conserved gene sequences. In the present study, we developed CISP to sequence partial genomic fragments of the KO gene from seven Poa species. Through sequence analysis, species-specific primers were also developed to produce co-dominant markers that can be used to identify interspecific hybrids between Texas bluegrass and six other Poa species used in the present study.

show abstract

“…Bor (1952) asserted that the genus is evolutionarily complex and taxonomically difficult, and Kellogg (1985) stated that morphological characters used for classification of species belonging to the genus are overlapping. Hybridization and polyploidization have also played a prominent role in the evolution of this complex genus (Patterson et al, 2005). Therefore, most studies focused on the classification and phylogeny of the group based on cpDNA (Soreng, 1990;Gillespie and Boles, 2001) or combination of cpDNA and nDNA (Patterson et al, 2005;Soreng et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Hybridization and polyploidization have also played a prominent role in the evolution of this complex genus (Patterson et al, 2005). Therefore, most studies focused on the classification and phylogeny of the group based on cpDNA (Soreng, 1990;Gillespie and Boles, 2001) or combination of cpDNA and nDNA (Patterson et al, 2005;Soreng et al, 2010). This study conducted the first attempt, however, to identify the targeted species of Poa.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of pyrosequencing for large-scale identificationof plant species (grasses as a model)

Haider¹

2015

Turk J Agric For

View full text Add to dashboard Cite

Grasses (Poaceae) are among the most important and widely cultivated plants. Identification of grass species using morphological characters is a difficult task for nonspecialists, and it is not always an accurate method. DNA-based molecular markers have been widely used as an alternative towards more accurate identification of plant species. The molecular method that can be effective for large-scale identification of grass species, however, must satisfy requirements for specific amplification of DNA and reveal enough variability to distinguish species. The goal of this study was to evaluate the ability of pyrosequencing to detect SNP polymorphisms for an optimal discrimination of grass species. PCR amplifications and the sequencing of 19 fragments of chloroplast genes were performed using a pooled DNA template of 32 British native grass species to be identified and chloroplast DNA-specific universal primers. Based on amplicon size and sequence variance, the 6 most variable loci (psbE&F, rbcL.1, ndhF.1, ndhF.2, ndhF.3, and clpP&rps12) were selected to be targeted for pyrosequencing of the species analyzed. The pyrosequenced regions contained sufficient polymorphisms to allow the complete diagnosis of all species analyzed, except for Elymus caninus and Elytrigia repens, which had identical sequences unique from the remaining species. When targeted loci were pyrosequenced in representative species of the 2 economically important grass genera Festuca L. (8 species) and Poa L. (11 species), all species in each genus could be separated. Should the SNP markers developed here proved to be species-specific, they can provide a valuable tool for reliable diagnosis of grasses and applications (e.g., forensic botany) moving forward.

show abstract

Genome relationships in polyploidPoa pratensisand otherPoaspecies inferred from phylogenetic analysis of nuclear and chloroplast DNA sequences

Cited by 46 publications

References 56 publications

Isolation and characterization of chromosomal markers in Poa pratensis

Isolation and characterization of chromosomal markers in Poa pratensis

Development of ent-kaurene Oxidase-Based Conserved Intron Spanning Primers for Species Identification in the Genus Poa (Poaceae; Bluegrass)

Evaluation of pyrosequencing for large-scale identificationof plant species (grasses as a model)

Contact Info

Product

Resources

About