Genome Properties of Bamboo Mosaic Virus

Lee

Proc. Natl. Acad. Sci. U.S.A.

et al. 1996

120

Section: Methodsmentioning

confidence: 99%

The open reading frame of bamboo mosaic potexvirus satellite RNA is not essential for its replication and can be replaced with a bacterial gene.

Lee

Proc. Natl. Acad. Sci. U.S.A.

et al. 1996

120

Journal of General Virology

“…1 C, lane 1 ; Lin et al, 1992), the virus particles of the BaMV-O isolate sedimented as a single UV-absorbing band in a sucrose density gradient after centrifugation (designated G) (Fig. 5 A).…”

Section: Sedimentation Of Bamv-l Isolate Particlesmentioning

confidence: 99%

“…The product of ORF5 is the capsid protein of 25 kDa. Genomic RNA and the two major subgenomic RNAs, which are about 2n0 and 1n0 kb in size, could be detected in inoculated protoplasts and plants (Lin et al, 1992(Lin et al, , 1996. Like subgenomic RNAs associated with other potexviruses, the 2n0 and 1n0 kb subgenomic RNAs may be responsible for expression of the ORF2 protein and capsid protein, respectively (White & Mackie, 1990).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Subgenomic RNAs of bamboo mosaic potexvirus-V isolate are packaged into virions.

Lee

Lin²,

Hsu³

et al. 1998

Purified virions of bamboo mosaic potexvirus-V isolate (BaMV-V) were found to contain three major RNA species, the 6n4 kb genomic RNA and two RNAs of 2n0 and 1n0 kb, in addition to associated satellite RNA (0n85 kb). Results of Northern blot hybridization, primer extension analysis and cDNA sequencing showed that the packaged 2n0 and 1n0 kb RNAs of BaMV-V were subgenomic RNAs. In contrast, in the BaMV-O isolate, only genomic RNA was packaged and encapsidated subgenomic RNAs were not detectable. The transcription initiation sites for

“…For co-IP, 20-fold diluted protein samples were used for immunoblot analysis with anti-P20, anti-TGBp1, anti-TGBp2, anti-HA (Chou et al, 2013), rabbit anti-actin (1/5000 dilution, provided by Y.-Y. Hsu), or anti-CP antibodies (Lin et al, 1992;Chou et al, 2013) together with anti-fibrillarin IgG, H-140 (sc-25397) (Santa Cruz Biotech), followed by staining with goat anti-rabbit IgG HRP secondary antibody (Abcam).…”

Section: Protein Analysismentioning

confidence: 99%

The Nucleolar Fibrillarin Protein Is Required for Helper Virus-Independent Long-Distance Trafficking of a Subviral Satellite RNA in Plants

et al. 2016

RNA trafficking plays pivotal roles in regulating plant development, gene silencing, and adaptation to environmental stress. Satellite RNAs (satRNAs), parasites of viruses, depend on their helper viruses (HVs) for replication, encapsidation, and efficient spread. However, it remains largely unknown how satRNAs interact with viruses and the cellular machinery to undergo trafficking. Here, we show that the P20 protein of Bamboo mosaic potexvirus satRNA (satBaMV) can functionally complement in trans the systemic trafficking of P20-defective satBaMV in infected Nicotiana benthamiana. The transgenederived satBaMV, uncoupled from HV replication, was able to move autonomously across a graft union identified by RT-qPCR, RNA gel blot, and in situ RT-PCR analyses. Coimmunoprecipitation experiments revealed that the major nucleolar protein fibrillarin is coprecipitated in the P20 protein complex. Notably, silencing fibrillarin suppressed satBaMV-, but not HV-, phloem-based movement following grafting or coinoculation with HV. Confocal microscopy revealed that the P20 protein colocalized with fibrillarin in the nucleoli and formed punctate structures associated with plasmodesmata. The mobile satBaMV RNA appears to exist as ribonucleoprotein (RNP) complex composed of P20 and fibrillarin, whereas BaMV movement proteins, capsid protein, and BaMV RNA are recruited with HV coinfection. Taken together, our findings provide insight into movement of satBaMV via the fibrillarin-satBaMV-P20 RNP complex in phloem-mediated systemic trafficking.