Genome Organization inBeauveria bassiana:Electrophoretic Karyotype, Gene Mapping, and Telomeric Fingerprint

Viaud, Muriel; Couteaudier, Yvonne; Levis, Caroline; Riba, Guy

doi:10.1006/fgbi.1996.0033

Cited by 58 publications

(57 citation statements)

References 28 publications

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“…St Leger et al (1992) and Maurer et al (1997) implicated insect host preference as a significant determinant of genetic similarity, where strong selective pressures imposed by insect hosts restrict the number of successful genotypes. Supporting data by Viaud et al (1996) and Couteaudier and Viaud (1997) found genetic homogeneity among isolates from Ostrinia nubilalis and implicated vegetative compatibility groups in defining the likelihood of genetic exchange. Two studies by Coates et al (2001Coates et al ( , 2002 indicated a weak association between genotype and insect host preference, which mirrored conclusions from Aspergillus (St Leger et al 2000).…”

Section: Introductionmentioning

confidence: 76%

“…Parasexuality and vegetative compatibility among B. bassiana was previously described (Paccola-Meirelles and Azevedo 1991; Couteaudier and Viaud 1997) and may contribute to genetic similarity among stains (Viaud et al 1996;Couteaudier and Viaud 1997). Genetic transfer between incompatible isolates, strains, or different Beauveria spp has not been shown directly, but heterokaryon formation was implicated in the genetic exchange of a transposon between Beauveria spp (Kempken et al 1998) and might explain group I intron switching among ITS region haplotypes (Fig.…”

Section: Intron Phylogeny and Mobility By Horizontal Transfermentioning

confidence: 86%

“…St Leger et al (1992) and Maurer et al (1997) suggested that insect host range affected genetic similarity among strains, with selection imposed by insect pathogen defense restricting the number of successful genotypes, but this was contradicted by data presented by Coates et al (2001Coates et al ( , 2002 and analogous data from Aspergillus (St Leger et al 2000). Viaud et al (1996) and Couteaudier and Viaud (1997) suggested that vegetative compatibility groups influenced genetic similarity among isolates and likelihood of genetic exchange, especially those from Ostrinia Beauveria group 1 intron sequence data generated in this study are marked with an asterisk, followed by internal transcribed spacer region haplotype (A, C, G, L, O), as determined by Coates et al (2002). b Neighbor-joining distance tree using Jukes-Cantor model of DNA sequence evolution.…”

Section: Intron Phylogeny and Mobility By Horizontal Transfermentioning

confidence: 96%

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Nuclear small subunit rRNA group I intron variation among Beauveria spp provide tools for strain identification and evidence of horizontal transfer

2002

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An optional group I intron was characterized at a single insertion point in nuclear small subunit rRNA (nuSSU rRNA) genes of the imperfect entomopathogenic fungi, Beauveria bassiana and B. brongniartii. Insertion points were conserved among nuSSU rRNA genes from 35 Beauveria isolates. PCR-RFLP and DNA sequencing identified 12 group I intron variants and were applied to the identification of strains isolated from insect hosts. Alignment of 383-404-nt subgroup IB3 group I introns indicated that four insertion/deletion (indel) mutations were the main basis of fragment length variation. Phylogeny reconstruction using parsimony and neighbor-joining methods suggested six lineages may be present among nuSSU rRNA group I intron sequences from Beauveria and related ascomycete fungi. Terminal node placement of Beauveria introns conflicted with previously published phylogenies constructed from gene sequences, suggesting horizontal transfer of group I introns. PCRRFLP among introns provided a means for the differentiation of Beauveria isolates. Abstract An optional group I intron was characterized at a single insertion point in nuclear small subunit rRNA (nuSSU rRNA) genes of the imperfect entomopathogenic fungi, Beauveria bassiana and B. brongniartii. Insertion points were conserved among nuSSU rRNA genes from 35 Beauveria isolates. PCR-RFLP and DNA sequencing identified 12 group I intron variants and were applied to the identification of strains isolated from insect hosts. Alignment of 383-404-nt subgroup IB3 group I introns indicated that four insertion/deletion (indel) mutations were the main basis of fragment length variation. Phylogeny reconstruction using parsimony and neighbor-joining methods suggested six lineages may be present among nuSSU rRNA group I intron sequences from Beauveria and related ascomycete fungi. Terminal node placement of Beauveria introns conflicted with previously published phylogenies constructed from gene sequences, suggesting horizontal transfer of group I introns. PCR-RFLP among introns provided a means for the differentiation of Beauveria isolates.

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Section: Introductionmentioning

confidence: 76%

Section: Intron Phylogeny and Mobility By Horizontal Transfermentioning

confidence: 86%

Section: Intron Phylogeny and Mobility By Horizontal Transfermentioning

confidence: 96%

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Nuclear small subunit rRNA group I intron variation among Beauveria spp provide tools for strain identification and evidence of horizontal transfer

2002

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“…This probe is therefore non-specific to B. cinerea, and it is likely that the observed signal with these other fungi is not due to the specific B. cinerea sequence, but to the TTAGGG repeats present in pTel13 making it a convenient tool for probing telomeric sequences (22). In the case of Beauveria bassiana, an entomopathogenic fungus, the use of pTel13 for fingerprint allowed to suggest that the observed polymorphism corresponds to the insect-host and not to the geographic origins of isolates (42). The presence of TTAGGG repeats and organization of the subtelomeric region of cromossomes of P. expansum and P. griseoroseum was checked using pTel13 as probe (Fig.…”

Section: Resultsmentioning

confidence: 99%

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Cardoso¹,

Queiroz²,

Pereira³

et al. 2007

Braz. J. Microbiol.

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Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. The only internal transcribed spacer region variation detected between the two species was the additional six initial nucleotides. The analysis by telomeric fingerprinting showed polymorphism between both species and the chromosome minimal numbers estimated were three. The polymorphism observed in the organization of the subtelomeric region in the genome of two Penicillium species within the high homogeneous Penicillium subgenus is for the first time reported and perhaps can be employed in future phylogenetic studies.

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“…Submerged conidia are also hydrophilic, but appear to be able to bind to both hydrophobic and hydrophilic surfaces, contain a rough surface morphology, and may be an important developmental adaptation for growth under nutrient limiting conditions. B. bassiana contains a haploid nuclear genome of between 34 and 44 Mb, consisting of 7-8 chromosomes, which by comparison to equivalent fungal genomes would contain 10 000-15 000 genes (Pfeifer & Khachatourians, 1993;Viaud et al, 1996). We have employed an expressed sequence tag (EST) analysis of aerial conidia, in vitro blastospores and submerged conidia in order to characterize the B. bassiana transcriptome.…”

Section: Introductionmentioning

confidence: 99%

EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. I. Evidence for stage-specific gene expression in aerial conidia, in vitro blastospores and submerged conidia

et al. 2006

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The entomopathogenic fungus Beauveria (Cordyceps) bassiana holds much promise as a pest biological control agent. B. bassiana produces at least three in vitro single cell infectious propagules, including aerial conidia, vegetative cells termed blastospores and submerged conidia, that display different morphological, biochemical and virulence properties. Populations of aerial conidia, blastospores and submerged conidia were produced on agar plates, rich liquid broth cultures and under conditions of nutrient limitation in submerged cultures, respectively. cDNA libraries were generated from mRNA isolated from each B. bassiana cell type and ∼2500 5′ end sequences were determined from each library. Sequences derived from aerial conidia clustered into 284 contigs and 963 singlets, with those derived from blastospores and submerged conidia forming 327 contigs with 788 singlets, and 303 contigs and 1079 contigs, respectively. Almost half (40–45 %) of the sequences in each library displayed either no significant similarity (e value >10−4) or similarity to hypothetical proteins found in the NCBI database. The expressed sequence tag dataset also included sequences representing a significant portion of proteins in cellular metabolism, information storage and processing, transport and cell processes, including cell division and posttranslational modifications. Transcripts encoding a diverse array of pathogenicity-related genes, including proteases, lipases, esterases, phosphatases and enzymes producing toxic secondary metabolites, were also identified. Comparative analysis between the libraries identified 2416 unique sequences, of which 20–30 % were unique to each library, and only ∼6 % of the sequences were shared between all three libraries. The unique and divergent representation of the B. bassiana transcriptome in the cDNA libraries from each cell type suggests robust differential gene expression profiles in response to environmental conditions.

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Genome Organization inBeauveria bassiana:Electrophoretic Karyotype, Gene Mapping, and Telomeric Fingerprint

Cited by 58 publications

References 28 publications

Nuclear small subunit rRNA group I intron variation among Beauveria spp provide tools for strain identification and evidence of horizontal transfer

Nuclear small subunit rRNA group I intron variation among Beauveria spp provide tools for strain identification and evidence of horizontal transfer

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. I. Evidence for stage-specific gene expression in aerial conidia, in vitro blastospores and submerged conidia

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