Genome of Bifidobacteria and Carbohydrate Metabolism

Bondue, Pauline; Delcenserie, Véronique

doi:10.5851/kosfa.2015.35.1.1

Cited by 25 publications

(17 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This species possesses the genetic machinery suggesting some ability to synthetize specific enzymes for BMO degradation, as highlighted by the presence of genes coding for β-galactosidase and α- or β-glucosidase, genes also present in other bacteria such as B. bifidum, B. longum subsp. infantis, B. mongoliense, B. biavatii, B. kashiwanohense , and B. stellenboschense (Delcenserie et al, 2007 ; Milani et al, 2014 , 2015 ; Bondue and Delcenserie, 2015 ). B. bifidum BBA1 grew as well on medium containing 3′SL as the main carbohydrate source.…”

Section: Discussionmentioning

confidence: 99%

Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3′-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium

Bondue¹,

Crèvecoeur²,

Brose³

et al. 2016

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Complex oligosaccharides from human milk (HMO) possess an antimicrobial activity and can promote the growth of bifidobacteria such as Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis. In addition, fermentation of carbohydrates by bifidobacteria can result in the production of metabolites presenting an antivirulence effect on several pathogenic bacteria. Whey is rich in complex bovine milk oligosaccharides (BMO) structurally similar to HMO and B. crudilactis, a species of bovine origin, is able to metabolize some of those complex carbohydrates. This study focused on the ability of B. bifidum and B. crudilactis to grow in a culture medium supplemented in 3′-sialyllactose (3′SL) as the main source of carbon, a major BMO encountered in cow milk. Next, the effects of cell-free spent media (CFSM) were tested against virulence expression of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium. Both strains were able to grow in presence of 3′SL, but B. crudilactis showed the best growth (7.92 ± 0.3 log cfu/ml) compared to B. bifidum (6.84 ± 0.9 log cfu/ml). Then, CFSM were tested for their effects on virulence gene expression by ler and hilA promoter activity of luminescent mutants of E. coli and S. Typhimurium, respectively, and on wild type strains of E. coli O157:H7 and S. Typhimurium using RT-qPCR. All CFSM resulted in significant under expression of the ler and hilA genes for the luminescent mutants and ler (ratios of −15.4 and −8.1 respectively) and qseA (ratios of −2.1 and −3.1) for the wild type strain of E. coli O157:H7. The 3′SL, a major BMO, combined with some bifidobacteria strains of bovine or human origin could therefore be an interesting synbiotic to maintain or restore the intestinal health of young children. These effects observed in vitro will be further investigated regarding the overall phenotype of pathogenic agents and the exact nature of the active molecules.

show abstract

Section: Discussionmentioning

confidence: 99%

Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3′-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium

Bondue¹,

Crèvecoeur²,

Brose³

et al. 2016

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In particular, infants with a Bifidobacteria dominated microbiota have been shown to have less fucosylated oligosaccharides in their faecal samples compared to infants with Bacteroides dominated microbiota, suggesting the former’s preference for fucosylated oligosaccharide consumption [ 12 ]. In general terms, bifidobacterial genomes contain fewer genes encoding for the breakdown of complex polysaccharides [ 34 ] consistent with their preference for the degradation of HMOs, starch and starch hydrolysates, with the dietary intake of starch at 9 months of age having been shown to be positively correlated with the abundance of Bifidobacteria spp. [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Mothers Secretor Status Affects Development of Childrens Microbiota Composition and Function: A Pilot Study

et al. 2016

View full text Add to dashboard Cite

BackgroundOne mechanism by which early life environment may influence long term health is through modulation of the gut microbiota. It is widely accepted that the optimal source of nutrition in early life is breast milk, with Human Milk Oligosaccharides (HMOs) thought to play an important role in nourishing the developing microbiota. However, mothers with inactive secretor genes have altered HMO composition and quantities in their breast milk. In this pilot study we examine the influence of secretor status and breast-feeding on microbiota composition at 2 to 3 years of age.Methods37 children and 17 eligible mothers were recruited. Secretor status was determined from blood and saliva samples using hemagglutination inhibition technique and faecal microbiota composition was examined by 16S rRNA gene sequencing.ResultsSecretor status was determined for 28 eligible children with 20 being secretors (S, 71.4%). Eleven of the 17 mothers were secretors (S, 64.7%). Unweighted UniFrac distances were significantly associated with child secretor status (R2 = 0.069, p = 0.030) and with mother secretor status in children exclusively breastfed for at least 4 months (R2 = 0.167, p = 0.028), suggesting an influence on the presence/absence of microbes, with Prevotella not detected in samples from secretor children and children of secretor mothers. In children who were exclusively breast-fed for at least 4 months of life the abundance of the known HMO consumers Bifidobacterium were increased in the children of secretor mothers compared to non-secretor mothers. The relative abundance of an OTU related to Bacteroides plebeius, a bacterium noted for its capacity to utilise sulphated polysaccharides for growth, was decreased in these children.ConclusionsChild and mothers’ secretor status have an impact on childrens’ microbiota composition at 2 to 3 years of age.

show abstract

“…Phylogenetic and protein modelling studies have recently suggested that the bifidobacterial XFPK enzyme is related to that found in members of the Coriobacteriales order and that xfpK was horizontally transferred between (an ancestor of) these two groups 21,28,29 . Another interesting difference between bifidobacteria (and now possibly Coriobacteriales) and other bacteria is that they lack a number of enzymes, such as phosphofructokinase, which are crucial to control central carbohydrate metabolism 30 .…”

Section: Introductionmentioning

confidence: 99%

Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators

Lanigan

Kelly

Arzamasov

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Bifidobacteria resident in the gastrointestinal tract (GIT) are subject to constantly changing environmental conditions, which require rapid adjustments in gene expression. Here, we show that two predicted LacI-type transcription factors (TFs), designated AraQ and MalR1, are involved in regulating the central, carbohydrate-associated metabolic pathway (the so-called phosphoketolase pathway or bifid shunt) of the gut commensal Bifidobacterium breve UCC2003. These TFs appear to not only control transcription of genes involved in the bifid shunt and each other, but also seem to commonly and directly affect transcription of other TF-encoding genes, as well as genes related to uptake and metabolism of various carbohydrates. This complex and interactive network of AraQ/MalR1-mediated gene regulation provides previously unknown insights into the governance of carbon metabolism in bifidobacteria.

show abstract

Genome of Bifidobacteria and Carbohydrate Metabolism

Cited by 25 publications

References 44 publications

Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3′-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium

Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3′-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium

Mothers Secretor Status Affects Development of Childrens Microbiota Composition and Function: A Pilot Study

Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators

Contact Info

Product

Resources

About