Genome mining Streptomyces sp. KCTC 0041BP as a producer of dihydrochalcomycin

Nguyen, Chung Thanh; Bridget, Adzemye Fovennso; Pham, Van Thuy Thi; Nguyen, Hue Thi; Kim, Taesu; Sohng, Jae Kyung

doi:10.1007/s00253-021-11393-w

Cited by 6 publications

(2 citation statements)

References 58 publications

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“…Further studies need to be pursued to determine the correlation between the biological activities of polyene and the presence of sugar moiety. Although the bioassay of I-NTF aglycone didn’t showed any visible antibacterial activity, other studies on linearmycin, of which structure is quite similar with I-NTF aglycone, was reported to maintain antibacterial activity without sugar moiety ( Figure 4 , Nguyen et al, 2021 ). Further studies will follow to elucidate the antibacterial mechanism of I-NTF as well as the significance of the sugar moiety of I-NTF.…”

Section: Discussionmentioning

confidence: 95%

BAC cloning and heterologous expression of a giant biosynthetic gene cluster encoding antifungal neotetrafibricin in streptomyces rubrisoli

Park

Kim

et al. 2022

Front. Bioeng. Biotechnol.

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Polyene natural products including nystatin A1, amphotericin B, ECO-02301, and mediomycin belong to a large family of valuable antifungal polyketide compounds typically produced by soil actinomycetes. A previous study (Park et al., Front. Bioeng. Biotechnol., 2021, 9, 692340) isolated Streptomyces rubrisoli Inha501 with strong antifungal activity and analyzed a large-sized biosynthetic gene cluster (BGC) of a linear polyene compound named Inha-neotetrafibricin (I-NTF) using whole genome sequencing and bioinformatics. In the present study, an entire I-NTF BGC (∼167 kb) was isolated through construction and screening of Streptomyces BAC library. Overexpression of the cloned I-NTF BGC in the wild-type S. rubrisoli Inha501 and its heterologous expression in S. lividans led to 2.6-fold and 2.8-fold increase in I-NTF yields, respectively. The qRT-PCR confirmed that the transcription levels of I-NTF BGC were significantly increased in both homologous and heterologous hosts containing the BAC integration of I-NTF BGC. In addition, the I-NTF aglycone-producing strains were constructed by a target-specific deletion of glycosyltransferase gene present in I-NTF BGC. A comparison of the in vitro biological activities of I-NTF and I-NTF aglycone confirmed that the rhamnose sugar motif of I-NTF plays a critical role in both antifungal and antibacterial activities. These results suggest that the Streptomyces BAC cloning of a large-sized natural product BGC is a valuable approach for natural product titer improvement and biological activity screening of natural product in actinomycetes.

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Section: Discussionmentioning

confidence: 95%

BAC cloning and heterologous expression of a giant biosynthetic gene cluster encoding antifungal neotetrafibricin in streptomyces rubrisoli

Park

Kim

et al. 2022

Front. Bioeng. Biotechnol.

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“…The cost of genome sequencing has decreased rapidly, due to the invention of nextgeneration sequencing (NGS) technologies, e.g., the Pacific Biosciences Single Molecule Real-Time (PacBio SMRT) platform [42]. Particularly, the Illumina short-read-based genome assemblies frequently missed large fragments of giant genes encoding PKS and NRPS, which play an important key role in studying the secondary metabolites of fungi [43].…”

Section: Discussionmentioning

confidence: 99%

Genome Mining and Analysis of PKS Genes in Eurotium cristatum E1 Isolated from Fuzhuan Brick Tea

Guo

Chen

Liu

et al. 2022

JoF

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Eurotium cristatum as the dominant fungi species of Fuzhuan brick tea in China, can produce multitudinous secondary metabolites (SMs) with various bioactivities. Polyketides are a very important class of SMs found in E. cristatum and have gained extensive attention in recent years due to their remarkable diversity of structures and multiple functions. Therefore, it is necessary to explore the polyketides produced by E. cristatum at the genomic level to enhance its application value. In this paper, 12 polyketide synthase (PKS) genes were found in the whole genome of E. cristatum E1 isolated from Fuzhuan brick tea. In addition, the qRT-PCR results further demonstrated that these genes were expressed. Moreover, metabolic analysis demonstrated E. cristatum E1 can produce a variety of polyketides, including citreorosein, emodin, physcion, isoaspergin, dihydroauroglaucin, iso-dihydroauroglaucin, aspergin, flavoglaucin and auroglaucin. Furthermore, based on genomic analysis, the putative secondary metabolites clusters for emodin and flavoglaucin were proposed. The results reported here will lay a good basis for systematically mining SMs resources of E. cristatum and broadening its application fields.

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Genome Sequencing and Genetic Engineering Reveal the Contribution of Bacitracin Produced by Bacillus paralicheniformis CPL618 to Anti-Staphylococcus aureus Activity

et al. 2023

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Genome mining Streptomyces sp. KCTC 0041BP as a producer of dihydrochalcomycin

Cited by 6 publications

References 58 publications

BAC cloning and heterologous expression of a giant biosynthetic gene cluster encoding antifungal neotetrafibricin in streptomyces rubrisoli

BAC cloning and heterologous expression of a giant biosynthetic gene cluster encoding antifungal neotetrafibricin in streptomyces rubrisoli

Genome Mining and Analysis of PKS Genes in Eurotium cristatum E1 Isolated from Fuzhuan Brick Tea

Genome Sequencing and Genetic Engineering Reveal the Contribution of Bacitracin Produced by Bacillus paralicheniformis CPL618 to Anti-Staphylococcus aureus Activity

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