Genome mining reveals polysaccharide-degrading potential and new antimicrobial gene clusters of novel intestinal bacterium Paenibacillus jilinensis sp. nov

Abstract

BackgroundDrug-resistant bacteria has posed a great threat to animal breeding and human health, it is obviously urgent to develop new antibiotics that can effectively combat drug-resistant bacteria. The commensal flora inhabited in the intestines become potential candidates owing to the production of a wide range of antimicrobial substances. In addition, host genomes do not encode most enzymes needed to degrade dietary structural polysaccharides, the decomposition of these polysaccharides mainly depends on gut commensals-derived CAZymes.Resultswe report a novel species isolated from the chicken intestine, designated as Paenibacillus jilinensis sp. nov. and with YPG26T (=CCTCC M2020899T) as type strain. The complete genome of P. jilinensis YPG26T consists of a single circular chromosome of 3.97 Mb in length, with a G+C content of 49.34% (mol %). It carries 33 rRNA genes, 89 tRNA genes, and 3871 protein-coding genes among which abundant carbohydrate degrading enzymes (CAZymes) are encoded. Moreover, this strain has capability to antagonize multiple pathogens in vitro, we identified putative 6 BGCs encoding bacteriocin, NRPs, PKs, terpenes and protcusin by genome mining. In addition, antibiotic susceptibility testing showed sensitivity to all antibiotics tested.ConclusionsThis study highlights the varieties of CAZymes genes and BGCs in the genome of Paenibacillus jilinensis. These findings confirm the beneficial function of gut microbiota, and also provide a promising candidate for the development of new carbohydrate degrading enzymes and antibacterial agents.