Genome mining of Streptomyces sp. CB00271 as a natural high‐producer of β‐rubromycin and the resulting discovery of β‐rubromycin acid

Abstract

β-rubromycin (β-RUB) ( 1) is an efficient inhibitor of human telomerase possessing a unique spiroketal moiety as a potential pharmacophore and regarded as a promising anticancer drug lead. But the development of (β-RUB) (1) has long been hampered

Discovery and Biosynthetic Studies of a Highly Reduced Cinnamoyl Lipid, Tripmycin A, from an Endophytic Streptomyces sp.

“…In our previous studies, CB04723 has already been identified as a producer of griseorhodins . Besides griseorhodin A and griseorhodin C, there were several other metabolites produced by CB04723 in MIE medium (Figure B).…”

“…In our previous studies, CB04723 has already been identified as a producer of griseorhodins. 36 Besides griseorhodin A and griseorhodin C, there were several other metabolites produced by CB04723 in MIE medium (Figure 1B). Based on UV absorption and molecular weight (Figure S5), these six major metabolites could be divided into two groups (Figure 1B).…”

“…Based on the antiSMASH analysis, CB04723 contained 27 putative secondary metabolite BGCs (Table S5). Among them, only two type II PKSs were found, in which #23 BGC was responsible for the biosynthesis of griseorhodins, while #7 BGC was a hybrid cluster containing one type II PKS. By comparing with mbg , a subcluster consisting of 32 open reading frames was located in #7 BGC and named hxs ( Figure ).…”

Characterization of Chalkophomycin, a Copper(II) Metallophore with an Unprecedented Molecular Architecture

“…CB00271, a high-yield producing strain of β-rubromycin and a new congener βrubromycin acid through a genome mining approach. 26 A green compound named chalkophomycin (CHM, 1) was isolated from the fermentation broth of S. sp. CB00271 with the addition of microporous resins (Figures S4−S9).…”

“…We have previously discovered S. sp. CB00271, a high-yield producing strain of β-rubromycin and a new congener β-rubromycin acid through a genome mining approach . A green compound named chalkophomycin (CHM, 1 ) was isolated from the fermentation broth of S. sp.…”