Genome Manipulations

Prakash, Shyam; Chopra, V. L.

doi:10.1007/978-3-662-06166-4_8

Cited by 1 publication

(2 citation statements)

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“…Similar complexity has been proposed for the genetic control of homoeologous pairing in AAC hybrids and homologous pairing associations in Brassica A-and C-genome haploids (Nicolas et al 2009). Reports of the frequency of allosyndetic pairing from traditional cytogenetic studies of AABC, BBAC and CCAB hybrids in the literature range from no allosyndetic pairing associations to frequent pairing (Morinaga 1929;Sasaoka 1930;U 1935;Harberd 1950;Prakash and Chopra 1993). We observed that AABC hybrids produced from three B. napus cultivars showed significant differences in frequency of genome-specific allosyndesis (A-B, A-C, B-C).…”

Section: Allosyndesismentioning

confidence: 60%

“…Homologous pairing disruption has not previously been reported in traditional cytogenetic studies of AABC hybrids (Morinaga 1929;Sasaoka 1930;Harberd 1950), CCAB hybrids (U 1935;Prakash and Chopra 1993) or BBAC hybrids (U 1935;Harberd 1950;Prakash and Chopra 1993). However, the ability to identify homologous pairing disruption in these studies was limited to observations of fewer than expected bivalents.…”

Section: Homologous Pairing Disruptionmentioning

confidence: 85%

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Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB Brassica interspecific hybrids

et al. 2010

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Gene introgression into allopolyploid crop species from diploid or polyploid ancestors can be accomplished through homologous or homoeologous chromosome pairing during meiosis. We produced trigenomic Brassica interspecific hybrids (genome complements AABC, BBAC and CCAB) from the amphidiploid species Brassica napus (AACC), Brassica juncea (AABB) and Brassica carinata (BBCC) in order to test whether the structure of each genome affects frequencies of homologous and homoeologous (both allosyndetic and autosyndetic) pairing during meiosis. AABC hybrids produced from three genotypes of B. napus were included to assess the genetic control of homoeologous pairing. Multi-colour fluorescent in situ hybridisation was used to quantify homologous pairing (e.g. A-genome bivalents in AABC), allosyndetic associations (e.g. B-C in AABC) and autosyndetic associations (e.g. B-B in AABC) at meiosis. A high percentage of homologous chromosomes formed pairs (97.5-99.3%), although many pairs were also involved in autosyndetic and allosyndetic associations. Allosyndesis was observed most frequently as A-C genome associations (mean 4.0 per cell) and less frequently as A-B genome associations (0.8 per cell) and B-C genome associations (0.3 per cell). Autosyndesis occurred most frequently in the haploid A genome (0.75 A-A per cell) and least frequently in the haploid B genome (0.13 B-B per cell). The frequency of C-C autosyndesis was greater in BBAC hybrids (0.75 per cell) than in any other hybrid. The frequency of A-B, A-C and B-C allosyndesis was affected by the genomic structure of the trigenomic hybrids. Frequency of allosyndesis was also influenced by the genotype of the B. napus paternal parent for the three AABC (B. juncea × B. napus) hybrid types. Homoeologous pairing between the Brassica A, B and C genomes in interspecific hybrids may be influenced by complex interactions between genome structure and allelic composition.

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Section: Allosyndesismentioning

confidence: 60%