Genome Insights into the Novel Species Jejubacter calystegiae, a Plant Growth-Promoting Bacterium in Saline Conditions

Abstract

Jejubacter calystegiae KSNA2T, a moderately halophilic, endophytic bacterium isolated from beach morning glory (Calystegia soldanella), was determined to be a novel species in a new genus in the family Enterobacteriaceae. To gain insights into the genetic basis of the salinity stress response of strain KSNA2T, we sequenced its genome using two complementary sequencing platforms (Illumina HiSeq and PacBio RSII). The genome contains a repertoire of metabolic pathways, such as those for nitrogen, phosphorus, and some amino acid metabolism pathways. Functional annotation of the KSNA2T genome revealed several genes involved in salt tolerance pathways, such as those encoding sodium transporters, potassium transporters, and osmoprotectant enzymes. Plant growth-promoting bacteria-based experiments indicated that strain KSNA2T promotes the germination of vegetable seeds in saline conditions. Overall, the genetic and biological analyses of strain KSNA2T provide valuable insights into bacteria-mediated salt tolerance in agriculture.

Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana

“…Bacteria produce a large number of secondary metabolites that act as a reservoir of bioactive metabolites, with some exhibiting unique functions such as salt resistance 37 . In the genus Subtercola , there are limited studies on the production of specialized metabolites and the potential source of natural products remains untapped.…”

“…Seeds of the Japonica rice cultivar Haepung were surface-sterilized with 1% (v/v) sodium hypochlorite for 10 min followed by repetitive washes with distilled water and soaked in AK-R2A1-2 T suspension (1 × 10 8 CFU/mL) or R2A broth as a control as described as Jiang et al 37 . The control and AK-R2A1-2 T bacteria-coated seeds were incubated in the dark at 14 °C for 4 days in three replicated square plates (245 mm × 245 mm) containing 0.15% water agar.…”

Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana

“…Bacteria can produce a large number of secondary metabolites that act as a reservoir of bioactive metabolites, with some exhibiting unique functions such as salt resistance 31 . In the genus Subtercola, there are limited studies on the production of specialized metabolites and the potential source of natural products in this genus remains untapped.…”

“…Seeds of the Japonica rice cultivar Haepung were surface-sterilized with 1% (v/v) sodium hypochlorite for 10 min followed by repetitive washes with distilled water and soaked in AK-R2A1-2 T suspension (1x10 8 CFU/mL) or R2A broth as control as described as Jiang et al 31 . The control and AK-R2A1-2 T bacteria coating seeds were incubated at 14°C of dark for 4 days in three replicated square plates (245 mm × 245 mm) which contained 0.15% water agar.…”

Microbial Diversity Associated with Photosynthetic Organisms

“…Microbial genome sequences have been a concern ever since the emergence of nextgenome sequencing. Jiang et al [13] investigated the complete genome sequence of the type strain of a plant growth-promoting bacterium Jejubacter calystegiae KSNA2 T (accession number KCTC 72234 T ) using two complementary sequencing platforms (Illumina HiSeq and PacBio RSII), and provided a framework for further genetic studies to better understand the mechanism of bacteria-mediated salt tolerance in plants. Functional annotation of the KSNA2 T genome revealed several genes involved in salt tolerance pathways, such as those encoding sodium transporters, potassium transporters, and osmoprotectant enzymes.…”