Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3

Abstract

Spore-forming Bacillus species are gaining interest in human health recently, due to their ability to withstand the harsh environment of the gastrointestinal tract. The present study explores probiotic features of Bacillus paranthracis strain MHSD3 through genomic analysis and in vitro probiotic assays. The draft genome of strain MHSD3 contained genes associated with tolerance to gastrointestinal stress and adhesion. Cluster genes responsible for the synthesis of antimicrobial non-ribosomal peptide synthetases, bacteriocins, and linear azole-containing peptides were identified. Additionally, strain MHSD3 was able to survive in an acidic environment, had the tolerance to bile salt, and exhibited the capability to tolerate gastric juices. Moreover, the isolate was found to possess strong cell surface traits such as high auto-aggregation and hydrophobicity indices of 79 and 54%, respectively. Gas chromatography–mass spectrometry analysis showed that the strain produced secondary metabolites such as amino acids, phenolic compounds, and organic acid, known to exert health-promoting properties, including the improvement of gastrointestinal tract health.

Bacillus altitudinis 1.4 genome analysis - functional annotation of probiotic properties and immunomodulatory activity

“…In this study, B. altitudinis 1.4 showed a rate of 47% on average. Lower results were found by [59] when evaluating the cellular hydrophobicity of a B. paranthrancis MHSD3 isolate, with a rate of 29%. In the ndings of [63] the percentage hydrophobicity of a B. amyloliquefaciens AMS1 isolate was 68%.…”

“…In 3 hours, the isolate B. altitudinis 1.4 showed 64.5% of auto aggregation. In the study by [59] results of 79% were found for the auto aggregation of Bacillus paranthrancis MHSD3.…”

Brevundimonas brasiliensis sp. nov.: a New Multidrug-Resistant Species Isolated from a Patient in Brazil

“…In our study, B. brasiliensis sp. nov. possessed an array of genes encoding virulence factors previously identified in many bacteria, and which are directly or indirectly related to biofilm formation ( carB [ 85 ], ricA [ 86 ], msrAB [ 11 ], and clpP [ 87 , 88 ]), adhesion ( cheB [ 87 ] and bpsC [ 89 ]), invasion ( fliP and fliI [ 90 ]; virB11 , [ 91 ]), LPS/O-antigen synthesis ( gmd [ 92 ]), synthesis of siderophore ( dhbA and dhbE [ 93 ]), and secretion and transport ( gspG [ 94 ] and xcpR [ 95 ]). Our strain also carried genes that encode isocitrate lyase ( icl ), elongation factor Tu ( tufA ), 2-dehydro-3-deoxyphosphooctonate aldolase ( kdsA ), and heat shock protein ( htpB ).…”

In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics

“…Autoaggregation, coaggregation and hydrophobicity of bacteria are considered to be important properties affecting their colonization in the intestine (Diale et al, 2021). Autoaggregation is the ability of the same cell types to self-adhere, and coaggregation is the combination of organisms of different species (Del Re et al, 2000;Kolenbrander, 2000).…”