Genome Functional Analysis of the Psychrotrophic Lignin-Degrading Bacterium Arthrobacter sp. C2 and the Role of DyP in Catalyzing Lignin Degradation

Jiang, Cheng; Yan, Haohao; Shen, Xiaohui; Zhang, Yuting; Wang, Yue; Sun, Shanshan; Jiang, Hanyi; Zang, Hailian; Zhao, Xinyue; Hou, Ning; Wang, Liwen; Wang, Hanjun; Li, Chunyan

doi:10.3389/fmicb.2022.921549

Cited by 5 publications

(4 citation statements)

References 52 publications

(87 reference statements)

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“…One-way ANOVA was used to compare data between different groups with a significance level of p < 0.05 or p < 0.001 ( Zhao et al, 2022 ). The relevant data in this study were analyzed by SPSS software 22.0 ( Jiang et al, 2022 ; Li et al, 2023 ). The Mantel test was performed with R4.2.1.…”

Section: Methodsmentioning

confidence: 99%

Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response

et al. 2023

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Triclocarban (TCC), an emerging organic contaminant (EOC), has become a severe threat to soil microbial communities and ecological security. Here, the TCC-degrading strain Rhodococcus rhodochrous BX2 and DCA-degrading strain Pseudomonas sp. LY-1 (together referred to as TC1) were immobilized on biochar to remove TCC and its intermediates in TCC-contaminated soil. High-throughput sequencing was used to investigate the microbial community structure in TCC-contaminated soil. Analysis of co-occurrence networks was used to explore the mutual relationships among soil microbiome members. The results showed that the immobilized TC1 significantly increased the removal efficiency of TCC from 84.7 to 92.7% compared to CK (no TC1 cells on biochar) in 10 mg/L TCC liquid medium. The utilization of immobilized TC1 also significantly accelerated the removal of TCC from contaminated soil. Microbial community analysis revealed the crucial microorganisms and their functional enzymes participating in TCC degradation in soil. Moreover, the internal labor division patterns and connections of TCC-degrading microbes, with a focus on strains BX2 and LY-1, were unraveled by co-occurrence networks analysis. This work provides a promising strategy to facilitate the bioremediation of TCC in soil, which has potential application value for sustainable biobased economies.

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Section: Methodsmentioning

confidence: 99%

Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response

et al. 2023

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“…Hu109A 5,131,917 62.60 – 4689 4097 (COG) 3577 (GO) 2681(KEGG) [ 69 ] Enterobacter lignolyticus SCF1 4,814,049 57.02 – – 3743 (COG) [ 14 ] Arthrobacter sp . C2 4,555,954 66.23 – – – [ 31 ] Arthrobacter sp . strain RT-1 4,545,853 65.69 282,801 – 4068 (KEGG) [ 70 ] Acinetobacter calcoaceticus CA16 4,110,074 38.69 – 3798 – [ 71 ] Burkholderia sp .…”

Section: Lignin-degrading Bacteriamentioning

confidence: 99%

Bacterial transformation of lignin: key enzymes and high-value products

Gu,

Qiu,

et al. 2024

Biotechnol Biofuels

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Lignin, a natural organic polymer that is recyclable and inexpensive, serves as one of the most abundant green resources in nature. With the increasing consumption of fossil fuels and the deterioration of the environment, the development and utilization of renewable resources have attracted considerable attention. Therefore, the effective and comprehensive utilization of lignin has become an important global research topic, with the goal of environmental protection and economic development. This review focused on the bacteria and enzymes that can bio-transform lignin, focusing on the main ways that lignin can be utilized to produce high-value chemical products. Bacillus has demonstrated the most prominent effect on lignin degradation, with 89% lignin degradation by Bacillus cereus. Furthermore, several bacterial enzymes were discussed that can act on lignin, with the main enzymes consisting of dye-decolorizing peroxidases and laccase. Finally, low-molecular-weight lignin compounds were converted into value-added products through specific reaction pathways. These bacteria and enzymes may become potential candidates for efficient lignin degradation in the future, providing a method for lignin high-value conversion. In addition, the bacterial metabolic pathways convert lignin-derived aromatics into intermediates through the “biological funnel”, achieving the biosynthesis of value-added products. The utilization of this “biological funnel” of aromatic compounds may address the heterogeneous issue of the aromatic products obtained via lignin depolymerization. This may also simplify the separation of downstream target products and provide avenues for the commercial application of lignin conversion into high-value products.

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“…However, the high carbon/nitrogen (C/N) ratio of straw and the presence of polymers such as cellulose and lignin hinder its degradation. In regions with cold temperatures, such as northern China, high latitudes and climate change further reduce straw degradation [ 8 , 9 , 10 ]. Undecomposed straw can affect crop germination and root growth, as well as subsequent crop yield and quality [ 11 ], and provides a favorable environment for pathogen proliferation and pest egg survival.…”

Section: Introductionmentioning

confidence: 99%

Straw Soil Conditioner Modulates Key Soil Microbes and Nutrient Dynamics across Different Maize Developmental Stages

Zhang,

Ye,

Chang

et al. 2024

Microorganisms

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Soil amendments may enhance crop yield and quality by increasing soil nutrient levels and improving nutrient absorption efficiency, potentially through beneficial microbial interactions. In this work, the effects of amending soil with straw-based carbon substrate (SCS), a novel biochar material, on soil nutrients, soil microbial communities, and maize yield were compared with those of soil amendment with conventional straw. The diversity and abundance of soil bacterial and fungal communities were significantly influenced by both the maize growth period and the treatment used. Regression analysis of microbial community variation indicated that Rhizobiales, Saccharimonadales, and Eurotiales were the bacterial and fungal taxa that exhibited a positive response to SCS amendment during the growth stages of maize. Members of these taxa break down organic matter to release nutrients that promote plant growth and yield. In the seedling and vegetative stages of maize growth, the abundance of Rhizobiales is positively correlated with the total nitrogen (TN) content in the soil. During the tasseling and physiological maturity stages of corn, the abundance of Saccharimonadales and Eurotiales is positively correlated with the content of total carbon (TC), total phosphorus (TP), and available phosphorus (AP) in the soil. The results suggest that specific beneficial microorganisms are recruited at different stages of maize growth to supply the nutrients required at each stage. This targeted recruitment strategy optimizes the availability of nutrients to plants and ultimately leads to higher yields. The identification of these key beneficial microorganisms may provide a theoretical basis for the targeted improvement of crop yield and soil quality. This study demonstrates that SCS amendment enhances soil nutrient content and crop yield compared with conventional straw incorporation and sheds light on the response of soil microorganisms to SCS amendment, providing valuable insights for the future implementation of this material.

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Genome Functional Analysis of the Psychrotrophic Lignin-Degrading Bacterium Arthrobacter sp. C2 and the Role of DyP in Catalyzing Lignin Degradation

Cited by 5 publications

References 52 publications

Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response

Enhanced bioremediation of triclocarban-contaminated soil by Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 immobilized on biochar and microbial community response

Bacterial transformation of lignin: key enzymes and high-value products

Straw Soil Conditioner Modulates Key Soil Microbes and Nutrient Dynamics across Different Maize Developmental Stages

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