Genome flux in tomato cell clones cultured in vitro in different physiological equilibria. II. A RAPD analysis of variability

Bogani, Patrizia; Simoni, Alessandra; Lió, Píetro; Scialpi, A.; Buiatti, M.

doi:10.1139/g96-107

Cited by 36 publications

(12 citation statements)

References 17 publications

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“…However, genetic analysis of such traits is often difficult, and their genetic causes remain unknown. Both methods reveal changes in the DNA nucleotide sequence and, in particular, neutral mutations [8][9][10][11]. We have previously observed that, although the ploidy changes in cultured pea cells, regenerated plants preserve the original ploidy, and phenotypic changes are not associated with considerable rearrangements of the karyotype [7].…”

Section: Introductionmentioning

confidence: 99%

RAPD and ISSR analyses of regenerated pea Pisum sativum L. plants

et al. 2005

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Long-term pea callus cultures of different genotypes (mutants R-9 and W-1 and cultivar Viola) were used to regenerate plants (generation R 0 ). The regenerants displayed changes both in qualitative and in quantitative traits. The most dramatic morphological alterations and complete sterility were observed in regenerants of the cultivar Viola. To estimate the genetic differences, regenerants were compared with the original lines with the use of RAPD (random amplified polymorphic DNA) and ISSR (inter simple sequence repeat) analyses. The extent of divergence varied among regenerants and depended mostly on the original genotype. The genetic difference from the original line was no more than 1% in W-1 regenerants, 0.7-5.3% in R-9 regenerants, and 10-15% in sterile regenerants of the cultivar Viola. The genetic variation of plants regenerated from a callus culture maintained for ten years did not exceed that of plants obtained from a culture maintained for two years. PLANT GENETICS

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Section: Introductionmentioning

confidence: 99%

RAPD and ISSR analyses of regenerated pea Pisum sativum L. plants

et al. 2005

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“…This indicates that, at a specific cytokinin/auxin combinations, adventitious regeneration system can induce variability in blackberry Č ačanska Bestrna. There are also some reports suggesting that different levels of auxin/cytokinin can affect the rate of somaclonal variation in strawberry cultures (Nehra et al 1992) and tomato long-term culture (Bogani et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

et al. 2010

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Adventitious shoot regeneration from leaves of blackberry cultivar Č ačanska Bestrna was examined using 30 different combinations of treatment. Young, fully expanded leaves taken from in vitro proliferating shoots were cultured on Murashige and Skoog (MS) medium containing either N 6 -benzylaminopurine (BAP) (2.0 mg l -1 ) or thidiazuron (TDZ) (1.0 and 2.0 mg l -1 ) alone, or either of them combined with indol-3-butyric acid (IBA), a-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) at different concentrations (0.1, 1.0 and 2.0 mg l -1 ). Indirect shoot formation was observed in 12 different treatments, though the efficacy varied greatly among types and concentrations of plant growth regulators. TDZ at 1.0 mg l -1 , applied either alone or combined with IBA, was significantly more effective than BAP in inducing shoot regeneration. The highest regeneration rate (41.66%) was obtained on medium containing TDZ alone. Cytological, flow cytometry and isozyme analyses were used for screening of genetic stability/instability in regenerants. Cytological study, based on chromosome counts in root tip meristems, and flow cytometry analysis indicated that adventitious shoots of Č ačanska Bestrna are tetraploid with 2n = 4x = 28 as well as those derived from axillary buds. However, a study conducted on the peroxidase patterns of the different blackberry regenerating lines showed differences between some lines and control plants (in vivo plants and micropropagated plants). These differences were visible with 3,3 0 ,5,5 0 -tetramethylbenzidine (TMBZ) as hydrogenous donor for peroxidase detection.

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“…Somatic embryo plants have generally been less variable, and that indicated the maintenance of genetic fidelity; however, so-mac10nal variation could be observed when somatic embryo cultures were kept for a longer time in in-vitra conditions (Henry et al, 1997). Bogani et al (1995Bogani et al ( , 1996 analysed genetic variation with RAPDs in tomato long-term cultures grown on media containing different auxinlcytokynin equilibria. Thus, subcloning in Haworthia (Ogihara, 1990), an increase in the number of propagation cycles in Begonia (Westerhof et al, 1984), and ageing of Begonia (CasseIls and Morrish, 1987) and Pelargonium cultures , all increased chromosomal and/or phenotypic variation among regenerated plants (Table 8).…”

Section: Frequency Modulation Of Somaclonal Variabilitymentioning

confidence: 99%

Somaclonal Variation and Induced Mutations in Crop Improvement

Jain¹,

Brar²,

Ahloowalia³

1998

Current Plant Science and Biotechnology in Agriculture

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Aims and ScopeThe book series is intended for readers ranging from advanced students to senior research scientists and corpomte directors interested in acquiring in-depth, state-of-the-art knowledge about research findings and techniques related to all aspects of agricultural biotechnology. Although the previous volumes in the series dealt with plant science and biotechnology, the aim is now to also include volumes dealing with animals science, food science and rnicrobiology. While the subject matter will relate more particularly to agricultural applications, timely topics in basic science and biotechnology will also be explored. Some volumes will report progress in rapidly advancing disciplines through proceedings of symposia and workshops while others will detail fundamental information of an enduring nature that will be referenced repeatedly.The titles published in this series are listed at the end ofthis volume.

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Genome flux in tomato cell clones cultured in vitro in different physiological equilibria. II. A RAPD analysis of variability

Cited by 36 publications

References 17 publications

RAPD and ISSR analyses of regenerated pea Pisum sativum L. plants

RAPD and ISSR analyses of regenerated pea Pisum sativum L. plants

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

Somaclonal Variation and Induced Mutations in Crop Improvement

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