Genome elimination in diploid and triploid Rana esculenta males: cytological evidence from DNA flow cytometry

Vinogradov, Alexander E.; Borkin, Leo J.; Günther, Rainer; Rosanov, Jury M.

doi:10.1139/g90-092

Cited by 75 publications

(82 citation statements)

References 17 publications

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“…Our GISH data applied to the whole P. esculentus genome correspond well with former studies on DNA content in erythrocytes of water frogs. Flow [Borkin et al, 1987;Vinogradov et al, 1990] and image [Ogielska et al, 2004] DNA cytometry showed that the P. ridibundus genome contains 10-16% more DNA than that of P. lessonae . Bi and Bogart [2006] reported stronger hybridization signals of GISH probes in the pericentromeric regions of all chromosomes in the Ambystoma hybrid complex.…”

Section: Discussionmentioning

confidence: 99%

Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization

Zaleśna

Choleva

Ogielska

et al. 2011

Cytogenet Genome Res

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The Western Palearctic water frogs Pelophylax ridibundus and P. lessonae were identified as parental (sexual) species and P. esculentus as their interspecific, hybridogenetically reproducing hybrid with hemiclonal heredity. We used genomic in situ hybridization (GISH) to identify parental chromosomes of P.lessonae and P.ridibundus in diploid P. esculentus karyotypes (2n = 26). GISH probes were made by fluorochrome labeling of total genomic DNA extracted from the sexual progenitors. The labeled probe from one species was hybridized to chromosomes of P. esculentus in the presence of excess of unlabeled genomic DNA from the other species. Thus, the P. lessonae probe was blocked by P. ridibundus unlabeled DNA, and vice versa. We successfully discriminated each of the 13 respective parental chromosomes in metaphase complements of the hybrids according to species-specific hybridization signals. GISH enabled us to confirm additional differences between parental chromosomes in size (smaller chromosomes belong to P. lessonae) and in the presence of DAPI-positive centromeric heterochromatin (detected in chromosomes of P. ridibundus, but not in P. lessonae). The fact that no visible intergenomic exchanges were found in metaphase chromosomes of diploid P. esculentus provides important information on the genomic integrity of hemiclonal transmission and supports hybridogenesis as a reproductive mode at the chromosome level for the specimens examined.

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Section: Discussionmentioning

confidence: 99%

Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization

Zaleśna

Choleva

Ogielska

et al. 2011

Cytogenet Genome Res

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“…esculenta but not in the other three species. VINOGRADOV et al (1990) based their studies on the finding that the -R. ridibunda genome contained 16 % more DNA than the R. lessonae genome. Perhaps this excess DNA is located mostly in the first and the largest chromosomal pair.…”

Section: Cytogenetic Analysismentioning

confidence: 99%

“…As we have said, in hybridogenetic reproduction one of the parental genomes is not transmitted to the next generation and is discarded before premeiotic synthesis of DNA (VINOGRADOV et al 1988;VINOGRADOV et al 1990). However, studies of the pattern of inheritance in diploid Rana kl.…”

Section: Introductionmentioning

confidence: 99%

Population composition and genetic variation of water frogs (Anura: Ranidae) from Yugoslavia

Spasić-Bošković

Krizmanić²,

Vujošević

1999

Caryologia

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-Species of the Rana kl. esculenta complex from twenty-one localities of the North-Western part of the Balkan Peninsula (Yugoslavia and Macedonia) were analyzed by electophoretic, morphometric and cytogenetic methods. The results of this research revealed that species that belong to the R. kl. esculenta complex from localities in Northern Yugoslavia form a ridibunda/esculenta/lessonae (R-E-L) population system of diploid forms of two parental species and predominantly female hybrids. Pure R. ridibunda populations exist south of the Sava and Danube Rivers. The unequal sex ratio observed in water frog population systems suggests, according to Haldane's rule, that the heterogametic sex suffers the effects of hybridization. Electrophoretic analysis shows that some introgression of ridibunda alleles into -R. lessonae, as well as lessonae into R. ridibunda has taken place. The occurrence of recombinations, and cytogenetic characteristics of the chromosome complements, does not support pure hybri-dogenesis as a general mode of reproduction in the hybrid form R. kl. esculenta from the examined localities.

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“…Similar processes occur in hybridogenetic vertebrates: in diploid hybridogenesis, one genome is transmitted clonally through the hybrid lineage, while the other is transmitted sexually by one of its parental species [6]. In meiotic hybridogenesis, both of the hybridizing genomes can be transmitted sexually, through crosses between diploid and triploid hybrids of different genomic compositions (figure 1; [14,16]). …”

Section: Introductionmentioning

confidence: 99%

Simultaneous Mendelian and clonal genome transmission in a sexually reproducing, all-triploid vertebrate

et al. 2011

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Meiosis in triploids faces the seemingly insuperable difficulty of dividing an odd number of chromosome sets by two. Triploid vertebrates usually circumvent this problem through either asexuality or some forms of hybridogenesis, including meiotic hybridogenesis that involve a reproductive community of different ploidy levels and genome composition. Batura toads (Bufo baturae; 3n ¼ 33 chromosomes), however, present an all-triploid sexual reproduction. This hybrid species has two genome copies carrying a nucleolus-organizing region (NORþ) on chromosome 6, and a third copy without it (NOR2). Males only produce haploid NORþ sperm, while ova are diploid, containing one NORþ and one NOR2 set. Here, we conduct sibship analyses with co-dominant microsatellite markers so as (i) to confirm the purely clonal and maternal transmission of the NOR2 set, and (ii) to demonstrate Mendelian segregation and recombination of the NORþ sets in both sexes. This new reproductive mode in vertebrates ('pre-equalizing hybrid meiosis') offers an ideal opportunity to study the evolution of non-recombining genomes. Elucidating the mechanisms that allow simultaneous transmission of two genomes, one of Mendelian, the other of clonal inheritance, might shed light on the general processes that regulate meiosis in vertebrates.

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Genome elimination in diploid and triploid Rana esculenta males: cytological evidence from DNA flow cytometry

Cited by 75 publications

References 17 publications

Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog <i>Pelophylax esculentus</i> by Genomic in situ Hybridization

Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog <i>Pelophylax esculentus</i> by Genomic in situ Hybridization

Population composition and genetic variation of water frogs (Anura: Ranidae) from Yugoslavia

Simultaneous Mendelian and clonal genome transmission in a sexually reproducing, all-triploid vertebrate

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